Pre-Class Activity, Week 5 - Gel Electrophoresis and Sanger Sequencing
This pre-class activity will help you think about the next step in the workflow:
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Name (last, first)
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Journal Club class next quarter?
We would like to know if you would be interested in participating next Quarter in a seminar course targeted to students who are currently in this class that focuses on building skills at reading scientific papers and also for giving talks/presentations. This would be a 2 unit seminar, letter grade, offered through the Freshman seminar series. We want to keep the momentum going, and continue to create a space where you can meet with your peers. Furthermore, we also want to help you further hone your research-related skills. Before we decide to go ahead and offer it, we need to know if there would be interest.
How likely are you to attend this class next quarter?
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Very likely - I will attend if at all possible
Pretty likely - Sounds interesting
Not likely
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In lab next week, we are going to see if your PCR worked by running your samples on a gel.
Watch the Gel Electrophoresis Video - you will actually make the gel yourself using an gelatin-like substance and a special mold
https://www.youtube.com/watch?v=u_WqMXmCi7c
Sanger video-
https://www.youtube.com/watch?v=e2G5zx-OJIw
Dilution calculation
You will be adding a dye to a buffer to make the gel in lab... We will want 50 ml of buffer solution (1X) The dye comes in a concentrate - 10,000X How much dye do you need to add to the solution? ***Hint:
https://www.youtube.com/watch?v=SWn__71BWNA
How much dye do you need to add make 50 mL of 1X solution?
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50 microliters (uL)
5 uL
.5 uL
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What is the purpose of the DNA Ladder in Gel Electrophoresis?
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We are going to be putting positive and negative controls on the Gel, why?
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Once the DNA fragments are in the capillary, how is their information translated into a computer file?
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