Week 6: PCR product clean-up and prep for sequencing
This pre-class activity will help you think about the next step in the workflow:
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In lab next week, we are going to Qubit DNA you extracted and then prep the samples for PCR. These samples will then get sent to Sanger sequencing.
Watch the Gel Electrophoresis Video - We are going to use a fancy pre-made gel in lab but typically you would actually make the gel yourself using an gelatin-like substance and a special mold
https://www.youtube.com/watch?v=u_WqMXmCi7c
Watch this Qubit video-
https://www.youtube.com/watch?v=ymny7xABaa4
PCR product clean-up kit video-
https://www.youtube.com/watch?v=eLOpCYBsQ4E
Sanger video-
https://www.youtube.com/watch?v=e2G5zx-OJIw
What is the reason for running samples through the Qubit?
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Why do we need dephosphorylation in our PCR clean up?
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Once the DNA fragments are in the capillary, how is their information translated into a computer file?
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