Careful Consideration of Bioinformatic Pipeline Choices Based on Study Goals

Sequencing of PCR-amplified marker regions (e.g. 16S, ITS) for characterization of sample microbial ecology is a widely-used tool in Microbiology of the Built Environmenta (MoBE) investigations. Due to the large amount of data produced by these methods, sequences are typically clustered into operational taxonomic units (OTUs) based on sequence similarity to simplify downstream processing. However, …