What sequencing core do you recommend for environmental samples with low concentrations?

Have you ever wondered what’s in the air of a daycare center, where kids swap microbes every day? A team from Virginia Tech and the University of Pittsburgh is investigating the viral ecology in air of a daycare center using metagenomics. We have collected samples on HVAC filters and are looking for a sequencing core …

Comparing the new 16S rRNA V4 and ITS primers to the old primers-RESULTS!

The Knight lab has been working hard testing new primers for 16S rRNA amplicon production and its time to share our progress. So far, the 16S rRNA V4 region forward primer (designated 515f) has been paired with five different reverse primers (806r, 926r, 967r, 1048r, and 1391r) to amplify ribosomal RNA from bacteria, Archaea, and …