So you want to run FACS on your microbiome samples…

So you want to run FACS on your microbiome samples… By Cara Pardon Although optimized for eukaryotic cell research, an increasingly common technique used in the microbiome field is Fluorescence Activated Cell Sorting (FACS).  From sorting cells for single cell genomics to identifying the host range of a plasmid; from detecting metabolically active cells in …

Two new papers exploring the characteristics of infant microbial exposures

Early-life microbial exposure has been recognized to have a crucial role in immune development. Exposure in the actual infant breathing zone (BZ), influenced by the resuspension of floor dust, however, is little explored. Two recently published papers tackle this issue by assessing infant exposure to biological particulate matter (fluorescent biological aerosol particles, FBAPs) and microbial …

Yes, gross, but interesting: Human skin flakes lead to bad smell in air-conditioning systems 

OK so this is based on a press release so it should be taken with a grain of salt and such.  But it is interesting, and gross too, and about microbiology of the built environment. Skin squames contribute to ammonia and volatile fatty acid production from bacteria colonizing in air-cooling units with odor complaints Source: …

Method of interest: Leaf-FISH: Microscale Imaging of Bacterial Taxa on Phyllosphere

This paper may be of use to people working on plant microbiomes. Peredo EL and Simmons SL (2018) Leaf-FISH: Microscale Imaging of Bacterial Taxa on Phyllosphere. Front. Microbiol. 8:2669. doi: 10.3389/fmicb.2017.02669 Abstract Molecular methods for microbial community characterization have uncovered environmental and plant-associated factors shaping phyllosphere communities. Variables undetectable using bulk methods can play an …

Worth a look: Filter forensics: microbiota recovery from residential HVAC filters

This is worth a look: Filter forensics: microbiota recovery from residential HVAC filters | Microbiome | Full Text From the paper Background Establishing reliable methods for assessing the microbiome within the built environment is critical for understanding the impact of biological exposures on human health. High-throughput DNA sequencing of dust samples provides valuable insights into …