We will be doing PCR to make more DNA of interest, we need this additional DNA for Sanger Sequencing.
In our PCR, we will be amplifying the 16S Ribosomal rRNA gene (this a gene name, we will be sequencing the DNA that is the blue print for this gene)
In class, you will be combining your samples with a Master Mix along with positive and negative controls.
After class, we will take your samples to the Eisen Lab and stick them in the Thermocycler. We will use this product in Week 5.
Week 5 will entail: (1) visualizing the PCR product on a gel , (2) cleaning the samples up with another commercial kit kit, (3) re-quantifying (Qubit again), and (4) diluting to appropriate concentration for sequencing.