Pre-Class Activity, Week 4 - PCR Prep

This pre-class activity will help you think about the next step in the workflow: PCR

We will be doing PCR to make more DNA of interest, we need this additional DNA for Sanger Sequencing.
In our PCR, we will be amplifying the 16S Ribosomal rRNA gene (this a gene name, we will be sequencing the DNA that is the blue print for this gene)

In class, you will be combining your samples with a Master Mix along with positive and negative controls.

After class, we will take your samples to the Eisen Lab and stick them in the Thermocycler. We will use this product in Week 5.

Week 5 will entail: (1) visualizing the PCR product on a gel , (2) cleaning the samples up with another commercial kit kit, (3) re-quantifying (Qubit again), and (4) diluting to appropriate concentration for sequencing.

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    We are going to start researching for the "begging for $$$ and stuff" project

    The research phase has 3 subphases 1. legal considerations: What kind of stuff can we get? 2. What stuff do we need? 3. Who might give us the stuff that we need? I need 3 teams to address these sub-phases
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    We would like to try the Protocol app: https://www.protocols.io

    Please make an account by following the link. https://www.protocols.io Join the Koala Poop group :)

    In lab next week, we are going to prep your DNA samples for PCR.

    1st Watch the Khan Academy video on PCR https://www.youtube.com/watch?v=aUBJtHwHASA 2nd Watch the “epic” PCR video to cement the concepts and pull all the ideas together from 1st video https://www.youtube.com/watch?v=iQsu3Kz9NYo
     
     
     
     
     
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