Pre-Class Activity, Week 5 - Gel Electrophoresis and Sanger Sequencing

This pre-class activity will help you think about the next step in the workflow:

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    Journal Club class next quarter?

    We would like to know if you would be interested in participating next Quarter in a seminar course targeted to students who are currently in this class that focuses on building skills at reading scientific papers and also for giving talks/presentations. This would be a 2 unit seminar, letter grade, offered through the Freshman seminar series. We want to keep the momentum going, and continue to create a space where you can meet with your peers. Furthermore, we also want to help you further hone your research-related skills. Before we decide to go ahead and offer it, we need to know if there would be interest.
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    In lab next week, we are going to see if your PCR worked by running your samples on a gel.

    Watch the Gel Electrophoresis Video - you will actually make the gel yourself using an gelatin-like substance and a special mold https://www.youtube.com/watch?v=u_WqMXmCi7c Sanger video-https://www.youtube.com/watch?v=e2G5zx-OJIw

    Dilution calculation

    You will be adding a dye to a buffer to make the gel in lab... We will want 50 ml of buffer solution (1X) The dye comes in a concentrate - 10,000X How much dye do you need to add to the solution? ***Hint: https://www.youtube.com/watch?v=SWn__71BWNA
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