Pre-Class Activity, Week 5 - Gel Electrophoresis and Sanger Sequencing

This pre-class activity will help you think about the next step in the workflow:

Name (last, first)

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Journal Club class next quarter?

We would like to know if you would be interested in participating next Quarter in a seminar course targeted to students who are currently in this class that focuses on building skills at reading scientific papers and also for giving talks/presentations. This would be a 2 unit seminar, letter grade, offered through the Freshman seminar series. We want to keep the momentum going, and continue to create a space where you can meet with your peers. Furthermore, we also want to help you further hone your research-related skills. Before we decide to go ahead and offer it, we need to know if there would be interest.

How likely are you to attend this class next quarter?

Very likely - I will attend if at all possible
Pretty likely - Sounds interesting
Not likely

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In lab next week, we are going to see if your PCR worked by running your samples on a gel.

Watch the Gel Electrophoresis Video - you will actually make the gel yourself using an gelatin-like substance and a special mold https://www.youtube.com/watch?v=u_WqMXmCi7c Sanger video-https://www.youtube.com/watch?v=e2G5zx-OJIw

Dilution calculation

You will be adding a dye to a buffer to make the gel in lab... We will want 50 ml of buffer solution (1X) The dye comes in a concentrate - 10,000X How much dye do you need to add to the solution? ***Hint: https://www.youtube.com/watch?v=SWn__71BWNA

How much dye do you need to add make 50 mL of 1X solution?

50 microliters (uL)
5 uL
.5 uL

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What is the purpose of the DNA Ladder in Gel Electrophoresis?

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We are going to be putting positive and negative controls on the Gel, why?

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Once the DNA fragments are in the capillary, how is their information translated into a computer file?

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