Updated storify of  session on The Great Indoors: Recent Advances in Ecology of Built Environments 
Haha! MT : Bacteria "invented everything; they probably invented animals and plants as a place to hang out." Rosenberg 
Up since 3 am PST, finally back home, sitting in lab grant writing and setting up cultures.   
   Yes it is. Gordon was PI. But one result among very big paper. 
: Ross says the bottled water she has surveyed has 100x as many microbes as tap water ” OH DEAR GOD.
Conf marathon day 12. At Logan airport ready to head back to the UK & work.  and  were great but
Ppl STILL buy marketing-water? RT : Ross says the bottled water she surveyed has 100x as many microbes as tap water
Had a brilliant time in California for  and microbiology conferences, both superb! Time to return to rainy Manchester, UK :(
Need to actually get my blog up and running with first entry summarizing my time at  have a tumblr but is there a better choice?
UNT/AMS collaboration identifies previously unknown bacterial group related to  removing  
Lots of comments on NPR's show on the human microbiome "Your Inner Ecosystem"   cc 
RT : Ross says the bottled water she has surveyed has 100x as many microbes as tap water 
Thanks everyone who stopped by our booth at  we enjoyed meeting you and learning about your research! See you next year!
 If true,  shd step up efforts to rectify the situation of disparities in minority representation in Micro 2/2
Yo tweeple who attended  Q: Is it me / was participation of African American women (grad student/postdoc) really low this time? 1/2
We had a great time at  hope you did too! If you missed any IdahoTech posters you can find them all here:
and we're still alive “ “ Ross says the bottled water she has surveyed has 100x as many microbes as tap water””
Ewww! RT : Ross says the bottled water she has surveyed has 100x as many microbes as tap water 
 Hope  is fun. Things are good, I manage and created our work twitter account so have become active.
More winners from : David Gambarzada, Chih-Ying Chen, Stacy Townsend-Great meeting U all. Congrats! Njoy & w 
 Great meeting you at ! Congrats on winning our gift card. We hope you Njoy it &  with :)
Great to hear so much about , almost like being there, maybe next year
But enough about the weather. Here’s what Daily Scan took from final day of , genomics in public health
can't be the only one bummed to have left the lovely, mild San Francisco weather only to return to a  in the Northeast
When it comes to the microbiome, we need to stop being microbial racists. It's time to include fungi in your analyses. -aks 
Norrine Thompson, Angelica Ruiz-Font: Great meeting U ! Congrats on winning our gift card. Njoy it &  w/ :)
RT : Ross says the bottled water she has surveyed has 100x as many microbes as tap water 
LA Times: Dust in homes with dogs may lower risk of infection linked to asthma  
 was very enjoyable. I had a chance to give a talk, made (I hope) some new friends, and shared laughs w/  & his posse.
This is a good idea for . a tweeps list would be great also! RT   I wish there was a participants list.
 Did you see David Hughes' "zombie ants" talk at? Phylo analysis => # times given ant behavior ctrl by fungi arose.
Another yuck inspiring tweet string from : Salads have 100M bacteria/g, mostly from manure  ht
I love being able to attend  virtually: "Bacteria invented animals as a place to hang out"  HT@erika_check
Take note! RT : NGS for outbreaks & public health symposium is srsly standing room only 
  thanks for the shout-off. It was a good meeting and Twitter is great for communicating interesting & notable
Just getting ready to board the Virgin America red-eye flight 2 Ft Lauderdale & I feel like one of those Zombie ants we saw today.
Following ? Get day by day highlights from our Live at ASM blog:  
Cal netejar l'amanida? RT : Levy: in salad, 100M bacteria per gram, mostly from manure. 
Last night in San Francisco with . 3 successful posters at  representing!
RT  Thanks ASM for making official space . Enjoyed and learned something. Safe journey back home to all the participa...
VIRUSES! These are the photos that a peer reviewer rejected because he was sure they were photoshopped  :
Thanks ASM for making official space . Enjoyed and learned something. Safe journey back home to all the participants! Join 
9,858 total, 6,682,scientists RT  Anyone have final number or estimate for  attendance?
cool to see  trending- way to go, microbiolotwitts!
so  ended tonight and I'm totally stoked to get back in the lab!!! 
 Very nice poster. How did you uploaded high resolution image? Are there any other posters you know of?
Only 15%  session 232 like the way ASM picks sessions/topics for the GM.
91%  session 232 attendees want to see ASM continue to experiment with social media
88% attendees at  symposium 232 think ASM should use social media to develop sessions
I noticed a large amount of microbiome research at compared with last year.
Thx,  for great tweets from . Love hearing about new bugs, discoveries. Wish I could learn to live tweet (and many other)
Had a lot of fun at my first ASM mtg  but i'm ready for it to be over and get home. So much to do in lab!
 Thanks for covering so much of the conference! Had fun following the talks I didn't get to via your tweets. 
 as I sit here in the lobby of Hotel Monaco with a glass of wine after a hard day tweeting, you inspire me! Good on ya.
Baker: know position of ClpXP on substrate sequence. AA composition and folding E vs transloc'n rate. Sequence dependence unclear. 
Baker: find long dwell times=unfolding substrate; rapid elong=translocation on unfolded polypeptide. Can meas. translocation rate 
: Hair worms in crickets. I can't unsee those videos, thanks David Hughes! 
Baker: have system to measure single molecule activity.Optically trap beads tethered to protease and substrate. Meas. displacement
Tania Baker: ClpXP is multisubunit chambered bacterial protease. post-translational control of signaling proteins; quality control
Belfort: inteins=self-splicing enz; free cysteine req. for hydrolysis. If cys at -3 position->ox disufide->inactive. Reduce->active 
Belfort: protein @ TI intron: cysteines coord. zinc->active intron. Oxidizing conditions-> disulfides -> No Zn ->inactive. Elegant.
: A Yersinia pestis upon the houses of poster tube thieves!” - uh-oh! Commiserations.
Marlene Belfort: fascinating talk on stress-responsive intron and intein regulators of bacteria. Type I and II both responsive.
BARTing it to the airport.  was great but exhausting! Good to meet so many tweeps.
A Yersinia pestis upon the houses of poster tube thieves! 
Martin: microbes are the 99.9999%. They occupy us! Why not the Freshman biology curriculum?  
Burkhardt: deletion of ~50 genes upreg in cold. Speculative mechanism: coli degrades mRNAs soaking up (inactive) ribosomes
David Burkhardt: characterize bacteria cold shock response w/ ribosome footprinting. Sees limited # genes up at 10C 
: Had an absolutely fab conference at  Now to source the funding so I can get to the next one...” --Amen!
How true. RT : Levy discussing  as societal & ecological drugs--you take them, they affect everyone 
 T Baker: Diff substrates vary in need for ATP saturation of Clp active sites.
Had an absolutely fab conference at  Now to source the funding so I can get to the next one...
Enjoying dinner after a great . Thanks everyone for stopping by the booth! See you at 
 yeah well that's all from me from ASM folks. It has been a great meeting .... but I am still off salad for the foreseeable future.
ESCRT proteins found in Archaea involved in viral egress; similar function also seen in Eukarya 
Dead cells (bacteria) inside apoptotic dead cells (Macs) inside cells. 
Seriously! RT  Tomography showing viral pyramids pushing up through the membrane of Infected cell
Pyramids form from ESCRT proteins, previously only known in eukaryotes. Help virus export elsewhere (e.g., Ebola) 
RT : These electron microscope shots are awesome. One reviewer accused them of photoshopping it. Straight lines.
 Tomography showing viral pyramids pushing up through the membrane of Infected cell 
 T Baker:seq specific variation in translocation rate of the unfolded substrate
These electron microscope shots are awesome. One reviewer accused them of photoshopping it. Straight lines. 
Does macrophage eating other apoptotic macs protect host in tuberculosis? Blocking it increases CFU 
This Yosemite virus forms 7-sided pyramids on cell surfaces, which burst and let virus out. Only such pyramids known in viruses.
RT  I would argue that Cyanobacteria have had the greatest impact on human health. 
 schadt's surveillance revealed huge amounts of poultry and porcine viruses on home refrigerator doors. Wash those hands folks!
 T Baker: Dual bead force clamp single molecule degradation assay
And so  is done, been a great meeting, had a fab time in
"How do you assemble a virus with a membrane on the inside?" (of the capsid) 
 T Baker: Multiple opportunities for regulation of clpXP deg through complexes
 admittedly 1 gave her bacterium a bikini but they do now know are smaller living things than ants!  2/2
Significant homology to eukaryotic RNA viruses in binding regions, none outside. 
RT : No known RNA viruses in Archaea. Until researchers started poking around hot springs in Yellowstone.
 yup.some 7-11yo made cuddly bacteria with me last wk.. 1/2
Enjoyed the your topics, your voice, your choice session at a nice mix of talks and def something that should happen at future mtgs
No known RNA viruses in Archaea. Until researchers started poking around hot springs in Yellowstone. 
Sam Behar discusses macs dying via necrosis and apoptosis in tuberculosis  
Like all good things,  event in San Francisco comes to a close! Great meeting, great presentations, info overload! /
 Tania Baker: ClpXP paradigm for energy dependent protein degradation.
Get’em while they’re young RT : Next up: on microbiology early in the undergrad curriculum 
 Lei Qi designed a CRISPR-based RNA processing system. Cool.
In search of archael viruses in Yellowstone 
 trumps news of  stroke in trending Twitter topics.
"Brute-force" pathogens vs "stealth" pathogens - gd descriptive terms 
Repeatability is uncanny. All lineages became virus-resistant via mutations in same gene: p=10^-22, incredibly unlikely by chance.
Viruses in S ocean survive by being temperate so they can hide out when bacteria are low and by having cold adapted genes
 so next up Eric Schadt and disease weather map. This has me intrigued.
Had so much fun   event in! Great meeting everyone & looking forward to the next event!
Elizabeth Perry, U Oregon, talking about repeatability of evolution in bacteria/phage systems. What makes evolution repeatable?
 Heading to Moscone Center to catch outflow. Thanks so much to all you tweeps for broadcasting the conference! Great job!
When I hear CRISPRs I always get hungry RT  David Paez-Espino - Time Series Metagenomics of CRISPRs 
: Is v impressive that can predict in such detail what ancestral bacteria shd have been like. ” --yup. 79% is amazing!
Is v impressive that can predict in such detail what ancestral bacteria shd have been like. 
Not a surprising outcome,  hinted at that a year or so ago. But nice t see the research proceeding. 
Blount sez Cit+ lineage in the Lenski long term evolution experiment is an incipient species, and a model organism for speciation.
Hm, coauthors should maybe discuss their talks. Same story/slides of Lujo virus as I saw yesterday 
For imaging people: you can quantify lots of things on images including colocalization and movement. Plz use more than "lots of"!
Comparison of genomic data reveals 3 virulence factors unique to deadly strains of Y pestis 
up soon: Tania Baker, a long time science hero of mine (protein degradation). First time I will have heard her talk. woo hoo!
"All models are wrong but some are useful" 
Bruce Rabin is the last award lecturer of . Hear his talk at 5 pm PT in the Esplanade Ballroom 309 ^lg
Yersinia pestis extracted from teeth and sequenced - paper also covered in  week 2 I think. 
Genome sequence of Y pestis extracted from teeth of plague victims compared to extant strains 
Jasmine Clark: nice images of env and gag transport and colocalization 
No, just following the tweets. ASV is next month :) RT : you at  too? I wish there was a participants list.
 David Baumler UW-Madison: coined term "paleo systems biology" -systematic look at ancient bugs, ancestors of modern pathogens
Yersinia pestis infection pictures - v grim hadn't realised still so common and still sometimes fatal 
 Are you at  too? I wish there was a participants list.
 I would argue that Cyanobacteria have had the greatest impact on human health. 
Thanks to all my microTweeple for the support; appreciate your coming to the talk. Hope you liked it; I believe in the topic! 
RT : RobertShafer: deep sequencing on viruses to ask if a single point mutation is relevant 
 More microbes into undergrad curriculum, and for the LOVE OF GOD get some protists in! Sick of getting blank stares about my field!
Debbie Lindell - Phages are a Selective Force Driving Cyanobacterial Genome Diversification 
 M. Belfort: Nature did redox trapping first
Rolla Daily News reports Missouri S&T researchers say tree oil may combat     
excellent question “: Microbes are the 99.9999% - why aren't they a bigger part of biology syllabus? 
E. coli K12 -- 2300 reactions 1200 metabolites 
Dedication! MT   Think  has a giant microbe on his lapel (I KNOW he has v small ones) /
 I jumped over to pathogen candidates and disease implication. Good crowd but I at least got a chair.
Just saw  talk about trying to teach Freshmen to become microbial literate. Great talk! 
Switching over to different session. Thomas Briese on Microbe hunting 
RT : Microbes are the 99.9999% - why aren't they a bigger part of biology syllabus? 
   we need an ASM commission to look at microbiology curriculum in freshman biology!
He does, Helicobacter RT   I think has a giant microbe on his lapel (I KNOW he has very small ones).
Microbes are the 99.9999% - why aren't they a bigger part of biology syllabus? 
  "change can be evolutionary or revolutionary" - grt quote!
Ian Glomsky: tracking neutrophils during anthrax infection using Cre-lox system. Nice use of the technology. 
I would buy it! "  please write that book!! 'Do sweat the small stuff'"
 "Microbes are the 99.9999%. Occupy the curriculum." 
 comic sans acceptable in a talk where The Fonz's quoted” -grt catch! I was so engrossed in the talk, didn't notice!
RT : Martin: has students "fisk" GRE biology review for errors for extra credit  I've had students blog errata for txtbks
  points out microbial literacy problems in 2010 GRE subject test guide for Biology, says MCAT guides are worse.
"fisk" is a blog term I was not expecting to hear in an asm talk mainstreaming the blogosphere
Martin: has students "fisk" GRE biology review for errors for extra credit 
Another lignocellulose degrader - job for JGI! RT time for a banana slug microbiome project? 
 comic sans is acceptable in a talk where The Fonz is quoted
Intro students to qs, biofilms, generate hypotheses 
Reovirus is the way to go! RT  VSV may be a prime candidate for cancer (cont) 
 uses CV biofilm procedure with P. putida. 24 wells allows statstical analysis 
 clearly for therapeutic use a highly adaptive virus is not going to be an ideal partner unless you can really control adaptation.
  is a grt teacher! I wish my undergrad teachers were as exciting. Were I an undergrad now, I'd b totally engaged.
Martin: also need to introduce more math analyses into what students are doing 
How low can you go ": Michael Metzker: Viral metagenomics using Illumina platform can detect pfu of <0.1"
The room is packed in this  session on experimental evolution. Such neat research.
 VSV may be a prime candidate for cancer therapy if it can be controlled to leave body and not adapt once it has killed cancer cells
Martin: discuss importance of biofilms, center for biofilm engineering for lots of examples 
Hothouse flowers a big problem good example to intrigue students
 I hate sitting on floor of session but I am too damned tired to stand any longer. That's me propped against the wall!
 I think  has a giant microbe on his lapel (I KNOW he has very small ones there).
Kids who go to university of puget sound get a pretty great education! 
  Preaching to the Prokaryotic Choir could be Preaching to the Bacterial Base!
 Marlene has moved from Wadsworth to SUNY-Albany
Martin: students use art & other media to do micro projects, increases learning and literacy 
Generalist viruses able to shift and grow in other hosts. 
 up next Marlene Belfort (rm 130 for those moving around)
Talking about his students and what they do. Very cool! 
Michael Metzker: Viral metagenomics using Illumina platform can detect pfu of <0.1 
Fluctuating environments favors generalist viruses. Homogeneity favors specialists. 
 avoiding extinction by adapting is often added by a " fluctuating" environment. In a homogeneous environ a stronger strain may win
 D. Burkhardt: after cold shock lots of translation past the stop codon.
Paradigm shifts, paradigm drifts key article by elio schaechter some key ideas and cool new topics, tech 
Um, NO. Polio is NOT extinct!  RT: Small pox, polio and rinderpest all extinct in the wild. 
Schaechter article in Microbe on paradigm shifts vol4no9 538 MM recommends 
 David Burkhardt, ribosome profiling during cold shock. Nice stuff.
I want some! ": Martin: mundane but effective: buttons and t shirts, slogan candy ("microbes rule"). "
  on pain medication, promises an interesting seminar” -dunno abt the disclaimer; indeed interesting
Martin: recommends Elio Schaecter paper, Microbe November 2009 "Paradigm Shifts, Paradigm Drifts" 
Martin Polz: how much microbial diversity do we really need to consider? how do we define a bacterial population? 
 viruses that don't change may just wait out change or hitch a ride to a better place!
Viruses often face environmental change. Adapt, wait it out or piggy back. 
Appeal to the unusual and new get them excited with slogans. PR is important! 
Martin: mundane but effective: buttons and t shirts, slogan candy ("microbes rule"). 
 viruses face lots of environmental changes sometimes within host, sometimes between host. They respond & adapt to change
  students arrive from high school with low levels of microbial literacy
Martin: notes many areas these topics could be introduced early in bio curriculum 
Michael Metzker:"use of next-gen  only limited by our imagination"  
: Next up:  on microbiology early in the undergrad curriculum ” - v engaging already; interesting video
Small pox, polio and rinderpest all extinct in the wild. 
Martin: video, no one knows what biofilm or quorum sensing are.
 wow what keeps viruses from extinction is standing room only.
Martin: video asking 1st yr students questions re micro, what is bacteria, what is archean, are bacteria good or bad, mixed results
"If microbial supremacy is wrong, I dont want to be right" MM
 Have any of these HMP people looking for enterotypes used diversity as a feature? Clustering depends on feature selection!
Multimedia! First student answer violated Pace's law, rest go downhill. 
My only undergrad microbiol was clin stuff in vet degree - I think I may be one of the students  is talking about!
No love for the micro from other areas of bio. I can relate to this as only micro on fac of 13 
Sure does. MT  contradict Peer Bork 2011 nature paper?  Rob Knight: no evidence for discrete enterotypes
Starts off on a good note - he may be high on painkillers 
Martin: Only micro his students get is as seniors 
Atmospheric microbes primarilybmaking membrane transporters and catabolic enzymes 
OH at  - some of the deep tag sequencing projects are actually starting to return the same exact sequence twice.
A great family tree! Thanks for sharing. RT : a surprise meeting with Dr. Stan Falkow 
Next up:  on microbiology early in the undergrad curriculum 
Atmospheric microbes dominated by Acinetobacter 
Jorg Vogel: describes novel mechanism by which bacteria regulate mRNA expression using small RNAs. in sugar pathway reg'n.
. You'll dig this talk: R. Mueller - the impacts of deforestation on soil fungi  (via )
NGS for outbreaks & public health symposium is srsly standing room only  
Lively talk by Bassler (past ASM president) using quorum sensing molecules as anti- infective drugs 
Talk on both shotgun and tag metagenomic analysis of environment is nice comparison of different aspects. 
RT : DH: genomics for early warning outbreak detection on a global scale must be standardized- lessons from PulseNet.
Hell yeah! RT  didn't you know? Microbiologists are the new rock stars! 
 Julian Davies - We KNOW what is needed to reduce antibiotic resistance, but implementation has been the great challenge
Comparison of DNA and RNA of atmospheric communities dont differ -- "everybody there is active" 
Next up  to tell us how to teach proper micro in introbio! Very impt topic IMHO 
Besseler talk on quorum sensing was great. Not a biochemist myself but she was very engaging speaker. 
 Patrick Duffy is giving an excellent presentation reviewing & differentiating malarial vaccines in trial.
DH: "molecular Esperanto" would let everyone talk about NGS in the same language :-) 
RM: Level a forest to increase fungal soil diversity. 
DH: genomics for early warning outbreak detection on a global scale must be standardized- lessons from PulseNet. 
  hate to tell you but we have now moved from Dengue to Malaria. Not viral but Duffy making nice vaccine presentation
DH: bench top sequencing machines make genomic epidemiology technically feasible for small & med labs 
Back to the lab...Thank you  tweeters for a great meeting!
 MAG Rabbani frm Dhaka, his 1st public lecture ever, does a good job of chronicling antibiotic resistnc in bug isolates frm hosp env
 speakers don't save the meat to the end of your talk and then rush through it at light speed when you are out of time. Frustrating.
Rebecca Mueller - Forests, farms and fungi: the impacts of deforestation on soil fungi  
Microbes in S Pac gyre form Mn-nodules, microbes cluster around nodules b/c nodules help with access to carbon 
German EHEC outbreak genome called  says Dan Hamsen at the 
CA: "...so we can reject the hypothesis that everything is everywhere". More evidence against  
Microbes that live within the S Pacific gyre face very low nutrient (carbon) environment 
+1 RT  Interesting and excellent talk from Emma Trantham about public engagement 
What's for dinner after ? Your badge unlocks a 10% food discount at SF's best Indian cuisine  Near Moscone
Finally catching some  tweets at asm RT : there are over 4 million Americans living in dengue risk areas.
JF: Argues that some systems are too complex to be studied in pure culture, "We can't get cultures to do this" 
 Dear microbiologists, Please plot growth curves on semi-log plots because bacteria GROW EXPONENTIALLY 
Attn : RT : just reminding people that is tweeting all kinds of cool disease-geekness from
Interesting and excellent talk from Emma Trantham about public engagement 
/waves RT  I think I spy  furiously tapping away live tweeting an interesting talk at   her abilities!
  you should consider using  to create seminar transcripts for posterity (one day fully citable!)
 Trantham: MRSA quilt at  Really interesting; it shd b a grt teaching tool.
Some hits from acid salt lakes similar to known extremophiles
JF discusses the interspecies relationships of SAR11 
DNA sequencing from acid salt lakes in W. Australia shows many novel bacterial species, heavily alpha and beta proteos 
I think I spy  furiously tapping away live tweeting an interesting talk at   her abilities!
Outbreaks: PG: whole-genome  the ultimate diagnostic & subtyping tool 
Jed Fuhrman speaks about the invisible hand that guides temporal changes in microbial diversity 
 Trantham: Belly button biodiversity project, armpit-pa-looza... Ewww, but so cool!!
Emma Trantham is making me think that I should do some public engagement sounds like fun 
Outbreaks: Peter Gerner-Smidt:in the future, look @ pathogens based on virulence profile/subtype rather than genus/species/genotype 
Talk on Public Engagement at  nice to hear a british accent! Long live public engagement
Por eso es q no me gustan las ensaladas! RT : Levy: in salad, 100M bacteria per gram, mostly from manure. 
 attendees- thanks for stopping by! For more on how easy Nextera sample prep is for small genome DNA: 
 Dengue became a US reportable disease in 2010 serotypes 1 and 2 most prevalent here. 3 and 4 have been detected here as well.
Randomly bumped into two people from grad school days roaming the expo hall! Small world.  
Trantham: describing the "deficit model" of science comm--fill brains with info. Vs dialogue model--discussion w public 
 there are over 4 million Americans living in dengue risk areas. Outbreaks in Texas Florida and Hawaii as well as US territories
Salt lakes in w. Australia very dynamic, dramatic changes in pH, depth, and salinity 
It's been a great meeting this year! Missed having you at it. 
Trantham: Why engage--dispel misconceptions, encourage next generation, recruit subjects, provide info, taxpayer funding, it's fun
 1% of world population has experienced Dengue - Azliyati Azizan. & still no vaccine and no effective antivirals yet.
RT : I hate that I now know this  RT: Levy: in salad, 100M bacteria/ gr, mostly from manure.
Sad to be missing  - so close but yet so far away.
An experiment in collaborative filtering at  -- a session designed by participants via online proposals and online voting
Now up: Emma Trantham on public engagement in the 21st century
Bonnie Bassler about to talk. How  interactions in bacteria can control pathogenic bacteria   
Eating "probiotic" chocolate wishing one of you biome-types would engineer chocolate with gut microbes that will make me leaner
Damn! Missed Kevin Foster's talk, but finally got to meet and have a chat with him     
Exhibit hall is now closed. Thanks for a great conference, everyone! Safe travels home! 
Got to meet  at her poster and chat about Listeria! And chicken slurry. 
 room 309 is configured for "discount coach seating". Close!!!
:I hate that I now know this  RT: Levy:in salad,100M bacteria per gram, mostly from manure. 
So torn between the "Limits" and "Your Choices" sessions.... How to choose? 
Fab day at  and won a kindle touch thanks to cell press looking 4ward to the your choice talks join us in RM 305
Great news : ) RT : Having busy time at signing up loads of new members including some very prestigious scientists
Is there any place online where I can see the posters presented at
I'm crashing , and I've already said hello to someone who's talk tweets I was following. Twitter works, people!
Big line at  earlier today to get in to Stuart Levy's talk on Antimicrobial resistance 
Larry Forney (U Idaho): vaginal microbiota in health and disease 
Janet Jansson (LBNL): Impact of perturbations on the human gut microbiome  
Proctor: Human Health and Microbial Ecology coming together with the Human Microbiome Project 
APHL staff, Chris Mangal, Sikha Singh&Sarah Muir-Paulik, present on public health lab activities at ASM.  
Rick Bushman (Penn): Dynamics of the human gut virome 
Last chance to see the most stable anaerobic workstation with the super cute name! The Bugbox is at booth 1534 until 2:45 pm!
Did everyone check out the IdahoTech booth at  ? Tweet a pic of your b. pertussis magnet if you snagged one!
Get highlights from  and up to the minute news from Twitter with our Live at ASM coverage: 
Microbes represented well at the SF exploratorium - brief escape from  
Jeff Chen  Raymond W. Sarber Award Undergraduate Laureate - WOW Bravo 
Only half of the mini  prizes have been claimed. Did anyone get this one? One hour left! 
 trending is further evidence of  rising popularity among scientists
Rob Knight  What is the impact of the loss in the Western World of parasitic eukaryotes that we have co-evolved with on
This will please you . RT : A scientific conference, , trending on .
 "Recursive Tuesday" - love it! Wish I were there this year  Eisen tweeting from ASM2011 got me started on twitter!
Hey  attendees. Check out our specific polyclonal STEC Antibodies. 
 hey with all these dozens of updates this ASM app does it should not have been a surprise a 1pm talk I trudged over for was canceld
Any  tweets interested in meeting up tonight?
Proctor: Microbiomes biobanked from cancer patients and readministered after chemotherapeutic treatment enables faster recovery. 
Interesting conversations coming out of dual use research panel — will blog. Stay tuned. 
: Huttenhower: linking microbiome to function. .^gm” could this research lead to personalised medicine
I'm at the Cell Press booth (1211) until 2, come by and chat if you're in the area 
Attending  this afternoon in San Francisco
Thanks ASM Live that allowed me to watch & interact with from a couch at  It is a great idea.
We get most of our fat cells before we're 5 years old. Could perturbed microbiome in early life "program" obesity later in life?
YK now discussing safety/security features of BSL3, BSL4 facilities. So cool! 
In future, it's possible that a course of antibiotics could be paired with probiotics. 
Hopefully we'll soon see consensus on microbiome biomarkers - which are causative and which are protective? 
Again, Ecology is stressed in the microbiome conversations (this time by Elizabeth Costello) 
Could antibiotic use in meat production contribute to obesity epidemic observed human population ? 
Last chance for Clin Micro mentoring today @ 1pm in the CM Lounge (112) - come chat with Patricia Cernoch and Dr. K. Johnson! ^tg
Re: therapeutic approaches for the , Lactobacillus and Bifidobacteria are the tip of the iceberg. 
YK: We believe  study is "important for pandemic preparedness." Information important for policy-makers, vaccine selection 
Stanford prof says we're still finding out about how the changes early in life. First 2 yrs seem to be important. 
Watching this great live chat on the human . Academics warn about  effects.  
Your bugs will love our Bugbox! The most stable anaerobic conditions and precise temp & humidity control. See it at booth 1534.
Laura Cox: Interplay between host anatomy + physiology and microbes  
Laura Cox: Increased environmental use of antibiotics throughout the latest decades may be causing the obesity epidemic 
  watch out for grapeseed extract, pomegranate polyphenol, cranberry proanthocyanins in coming days for anti-MRSA activity
Wow. That was a busy poster session. Great questions and even some lively debate 
Dual use research: Yoshihiro Kawaoka discussing results of his group's  experiments & conditions under which they were done 
Overheard at : "This is the future! This is the FUTURE!"
  study from Manitoba: hand washing awareness campaigns increase awareness of hygiene but doesn't change actual practice :(
 I'd be pushing them out of the way, Carvery is much more important than footy! haha  
 achievements: It was gratifying by one panel to see human health converged with microbiology studies. 
Last day of , don't forget to visit us-booth 1625. Last chance to meet, learn about   tool & get a gift
 achievements: Created computational tools for metagenomics studies.
The Brits at  seem to all be clustered around the carvery for some reason - apparently there is some sort of football match on...
Human  Project  RT achievements: Let people know bacteria are not all bad. They are part of human life.
 achievements: Had a profound impact on infectious disease studies. It is not just about single pathogen, but also a community.
Live in 10 Min -  Live – Antibiotic Exposure, The Microbiome & Obesity - Watch via 
MO: "The one agent that once it's out the door it's gone is influenza. ... There is no room for error." 
 achievements: Made people aware the facts that humans are animals, and embedded in (microbial) environments. 
Dual use research: MO: "Influenza is the one [accidental release] that scares the hell out of me." 
 doesn't that contradict Peer Bork's 2011 nature paper? Rob Knight: no evidence for "enterotype" clustering in Human Mic...
 achievements: a good drive to sequence many microbial communities and also detected 1000+ new proteins. 
Bonnie Bassler at American Academy of Microbiology mtg announcing "Microbes ca save the world" campaign 
Bonnie Bassler says the AAM is going to take a more prominent role in public discussion of microbiology 
 achievements from two panels: Generated a large number of datasets for hypothesis generation & data mining. 
Vaccine production for H1N1 flu was too little too late. Flu already burning out by time vac ready 
My question sent online was used as a wrap-up one for HMP Latest News session. What are the biggest achievements for the project?
Dual use research: MO: virulence potential of  has been a distraction — "not what this is really about" 
& the unrestricted publication of methods & results  2/2
At the American Academy of Microbiology luncheon Sitting at table w/ many  folks  
may never eat salad again MT :hate that I now know this RT:Levy:in salad 100M bacteria per gram,mostly from manure 
A centrifuge signed by the cart of CSI miami. Not impressive.
NC: lessons learned re:  research "enhanced policies & dialogue among governments, scientists, & civil society are necessary" 
  estrogen modulates course of UTI by uropathogenic E coli; Inc. susceptibility in postmenopausal women; case for HRT?
  Yay! Another mini MiSeq winner! There are still many to be found at the ASM conference at Moscone.
Dual use research: Nancy Cox: "public health pandemic risk assessment is limited to current knowledge" 
  Cryptococcus gattii (currently causing outbreak in pacific northwest) can create picture similar to metastatic lung cancer
we learned yesterday they transmit STDs. Whale mask & condom industry biz plan to follow:“: Um, whales can get influenza?
In responce to putting Pseudomonas on wounds  That could do more bad than good, no? Considering its a surface wound pathogen.
Thanks to  live for answering my question! Phage will be more important the more the microbiome project probes!
 U can sum what we learned here about state of our food industry w/: if a donut rolls across bathroom floor u might as well eat it!!
Dual use research: LE: just because experiments are 'of concern' doesn't mean we shouldn't do them. Precautionary measures key
    do not try what I did and find a corner in a poster session :(
More restaurant and entertainment discounts here: just show your  conf badge and enjoy. 
  very cool study on direct antimicrobial effect of human CC chemokines on leishmania amastigotes -1st report!
And so is your salad RT   Summary. Don't eat anything, don't touch anything. Your hotel room is feces contaminated.
 Summary: Don't eat anything; don't touch anything. Your hotel room is feces contaminated. Had great fun!
I hate that I now know this  RT : Levy: in salad, 100M bacteria per gram, mostly from manure.
20 min until  discussing EHEC paper - now to find somewhere at  to settle down and get paper out.
Dual use research: LE: process is flawed — bottleneck @ publication stage, after research already approved, funded, done & reviewed
 Are bacteriopahge looked at and identified in Faecal Microbiota Transplants, normally the information focuses on the bacteria
RT : Just in time for  study on bacteria in hotel rooms. Stay away from that TV remote!
Dual use research: journal editors as gatekeepers of potentially problematic papers pre-publication (LE) 
RT   Pam Silver just finished talking about engineering photosynthetic fish, magnetic yeast, other cool hacks.
 missed the live stream! Is it possible to watch it someplace else?? 
Dual use research: 2003 statement from major journals "recognized major change in attitudes about science publication" (LE) 
But our microbes may make us RT Don't eat anything, don't touch anything. Your hotel room is feces contaminated.
Using CRISPR spacers to modularize functional elements in engineered gene circuits, if I understand Adam Arkin correctly 
 Heratherm Microbio incubators have certified cleansing cycle & dual convection airflow 
Are you at  ? Stop by booth 1616 & enter to win Beats by Dr. Dre headphones. Pick up some free samples of our DNA & RNA kits too!
Last day to visit us at Booth 1131 for free samples and giveaways!
Lynn Enquist took helm at Journal of Virology in 2002: "a time of heightened concern about biological research," he says 
One eye on  , the other on  live and some how another on the  game (although this can take the back foot for now)
The smaller the organism, the more diverse & abundant. Most viruses being sequenced have no known homologs. How to characterize? 
that's not including the sprouts! I may be off all salad: “: Levy: in salad, 100M bacteria per gram, mostly from manure.
Found a sunny spot to warm up in after freezing conference rooms
Live in 10 min – The Latest News from the Human Microbiome Project - Watch here - 
That is Awesome!  : Just won a kindle thanks to the lovely ppl at cellpress!  
Just won a kindle thanks to the lovely ppl at cellpress! 
Headed home after great  &  mtgs. Been here for a week so will have to adjust to EST! Bye to my view!
last day for special giveaway at booth 1617 twitter promo 
  staph transports phenol-soluble modulin (toxin peptide), essential for virulence. transporter 'PMT' = putative drug target
Getting ready for session on recent H5N1 research controversy, controversy in science is fascinating, always makes you think
New  Barnstead LabTower EDI system combines purification w EDI technology & storage 
Hungry? I'm pleased to offer  attendees 10% food discount at my New Delhi Restaurant.  Just show your badge
Could you have tweeted this before lunch not after? RT : Levy: in salad, 100M bacteria per gram, mostly from manure.
 Sir! it is too small to read. Can you share online with some readability? 
Big drop in shaking hands! (or is that just people avoiding me?) RT People seem washing hands more carefully in bath room
Exhibit hall is open again! Demo the Ruskinn Bugbox at booth 1534 before you go. 
 Many  on antimicrobial esp. biofilm reducing action of plant based products like Tannins (green tea) & Curcumin (Turmeric)
Having busy time at  signing up loads of new members including some very prestigious scientists welcome to you all!
 Some *single-celled* algae (dinoflagellates) have an eye-like structure, complete with lens, retina, etc. 
People seem washing hands more carefully in bath room 
My faith in society is rewarded with  trending....the fact that it's trending with Alec Baldwin, well nothing's perfect!
 Bacteria have adaptive and innate immune systems (CRISPRs and restriction/modification system) 
  damn microbial cooperation! Pseudomonas & Staph help each other in Cystic Fibrosis lungs to enhance virulence of both!
Graham Hatfull's phage-hunter students name the viruses things like Patience, Predator, Konstantine; reminds me of hedgehog genes
 One of my micro students sampled a bag of "triple-washed" salad and plated onto TSA and was shocked at how much grew 
So it's the last day, and the exhibit hall is crazy, but here's my poster number (2194), come visit maybe?  
 Some amoeba are thought to have genomes 200x larger than human genome  
Fab! RT  is trending. This is an international meeting of microbiologists. (I am following via tweets this year)
KF: p aeruginosa only produces secreted rhamnolipids when there is excess carbon...when it's not costly 
Varaldi: phylogenetic analysis suggests Hytrosaviridae (near NUdiviridae and Baculoviridae). 
Hah! RT : Except Archaea RT: : Bacteria "invented everything...." Eugene Rosenberg, Tel Aviv U 
  antimicrobial Triclosan, ubiquitous in handwash, has undesirable effects: increases biofilm formn in pseudomonas & staph
Marie Pezzlo & Dr. Nachamkin will be here in the  Clin Micro Lounge (112) at 11am for Clin Micro Mentoring. Stop in - say hello! ^tg
Varaldi: concludes that viruses major players in behavior of host (insects, maybe more broadly?). NGS to survey breadth in flies
With all these Metagenomics approaches, will there be a new field “micro-ecology”? 
 Largest viruses have >5x more genes than smallest bacteria MT   trending again. some fun microbe facts?
 is trending. This is an international meeting of microbiologists. (I am following via tweets this year)
Julian Varaldi: parasitoid wasp driven by virus to superparasitism. Benefits virus > wasp. Transcriptional profiling for mechanism
File this under "things I didn't really need to know." :-P mT In salad, 100M bacteria per gram, mostly from manure.
65 genes different in virus-infected wasps; 3 looked at so far involved in neurotransmission 
Questions about human  project? Ask via twitter tag during ASM Live at 11:30 PDT today via 
Foster: You can generate strong selection dependent upon social structure 
Need reading material for your flight home from ? Last day to pick up a copy of your favorite  journal Booth 1308 ^nr
  next year we need Microbial Supremacy ribbons to hang from our name tags!
 find the mini  at the Moscone and bring it to booth 310 for a prize! Ready. Set. Go!!
  Shiga-toxin prod. E coli inf in which antibiotics are withheld in humans (bec. Increased morbidity) is amenable to oral Zn
If you have an interest in Burkholderia , come say hello to me at poster 2477. Rob 
Levy: in salad, 100M bacteria per gram, mostly from manure.
Microbes: the smaller they get the higher the amount of diversity 
Levy: 80% of antibiotics by weight given to animals for growth promotion. Enters into soils, streams, wildlife, people 
Spontaneous hypermucoid mutant. Spreads in colony, outcompetes, but not in liquid or in "mushed" colony 
  biofilm dispersal agents like D-amino acids enhance effect of antibiotics on bacterial biofilms 
 Jeff Miller "How do you move from correlation to causality?" The point of our NIH workshop a couple of years ago.
Levy: 1976 study looked at resistance. Found resistant bacteria in farm families & neighbors after using tet in chicken feed. 
RT : I love the story of fecal transplants treating chronic. Does that make me weird? 
SPACER



Graham Hatfull: if genomes are pervasively mosaic, how should we present their taxonomy? Uses dotplot of nucleotide seq similarity
 i love synthetic biology... amazing projects!.... really powerfull discipline!
  Glaxo SK reports in vitro efficacy of new polypeptide deformylase inhibitor active agnst key respiratory+skin bug incl MRSA
Spatial patterns emerge without evol pressure  in colonies
Hughes: able to preserve ant brains. Transcriptional profiling control vs. infected brains. Testing on generalist insect parasites 
Levy discussing antibiotics as societal & ecological drugs--you take them, they affect everyone 
 trending again. Perhaps it's time to put up some fun microbe facts for ppl clicking on the # to find out what it is?
Thx to everyone who visited our booth at . We love meeting our customers face-to-face!
Jumping to Stuart Levy talk on antibiotics  Freezing....
Chk out  lightweight performance from Fiberlite carbon fiber centrifuge rotors at booth 1015 
 Jeff Miller - Given HMP successes, what are next big questions and what tools do we need to go to next level?
90 minutes until   reading paper again during commute home
Hughes: histology-are fungi inducing lockjaw-type condition in dying leaf-biting ants? Says fungal family also ergotamine source.
 Interesting! Deep sequencing of the milk and milk product samples available in the market may answer this question 
": Bacteria "invented everything; they probably invented animals and plants as a place to hang out." Eugene Rosenberg, "
S phenotype of wasp correlates with viral infection 
Graham Hatfull: Fall semester = phage isolation, DNA extraction, sequencing; Spring = bioinformatics 
Well mixed structure makes evol cheating easier, spatial segregation gives cooperators advantage 
Why buy the conference cow when you can get the livetweet milk for free?   
  Trehalose biosynthesis pathway absent in humans, but regulates cell wall in Aspergillus fumigatus; future antifungal trgt?
Asimov: "The solar system consists of Jupiter, + debris." Me: the biosphere consists of microbes, + contaminants. 
Foster: family life is key to cooperative behavior. Giving him props for showing a really awkward family photo 
Parasitoid wasps of Drosophila that lay multiple eggs per larva (S phenotype) maternally transmitted 
Graham Hatfull: phages discovery & genomics = platform for research & education 
Foster plays to crowd with baby polar bear picture. 
Hughes: ant-infecting fungi drive amazingly precise behavior. Ants die facing N and W, at specific height, at noon. 
Kevin foster. From Oxford spatial structure and cooperation
Kevin Foster on bacterial social interactions 
Kevin Foster: It matters who you meet - Spatial structure and evolution of microbial cooperation 
Seems amazing that there would *not* be. RT  LF: change in vaginal community during menstruation for some women, not all 
Hughes: ...out swims impossibly huge worm that drove cricket to drown self. If cricket eaten by frog, worm can escape stomach.
Graham Hatfull: Genome-by-genome comparative analysis of phages reveals high diversity & genetic mosaicism 
Silver: photosynthetic zebra fish, transfected with cyanobacteria, but can't live on sunlight alone 
 Adam Arkin up next on synthetic bio (hm, I've seen those intro slides at least 3-4 times before; might reflect more on me than him)
David Hughes: parasitic fungi driving insect behavior. Video: dramatic example, worm-infected cricket jumps into water at night
LF: there is change in vaginal community composition during menstruation for some women, but not all 
  ingenious Gallium coordination Cx salts kill Pseudomonas in lung w/o the usual toxicities of reg Ga salts; grt for CF pts
Jumping sesssions again! now @ the interorganisms interactions session 
Delwart: salivirus--detection assoc with unexplained diarrhea in Nepal in case/control study, similar study in China 
Drosophila has two habitats: decaying fruit and vials in labs all over the world 
 Zombie ants was great talk! Am staying for parasitoid wasp talk. You? 
Graham Hatfull: exploration & exploitation of mycobacteriophages
  That should have read enteroviruses. I am being predictive texted & autocorrected to death by my iPhone.Grr!
 Pam Silver just finished talking about engineering photosynthetic fish, magnetic yeast, other cool hacks.
I love that Marraffini used pneumococcal capsule switching as model for testing CRISPR defense against acquiring virulence factor!
Delwart: cardioviruses. Mostly animal viruses but 80% adults seropositive 
 after enterocoruses cosaviruses may be 2nd most common viral enteric infection.
Maraffini: strep + CRISPR targeting capsule prevents capsule transformation and mouse death in repeat of Griffith 1928 
Studnts (all latinos) came 2 poster asked me about research, thn asked advice on gradschl.   
L maraffini: engineered strep with CRISPR targeting capsule. Repeated Griffiths 1928 mouse exp showing natural transformation
 both Bocavirus 1 and 2 have been detected in CSF in 4 cases encephalitis. Boca 1 usually associated respiratory & 2 in GI illness
Speak for yourself, I'm an ECOSYSTEM. MT  The Small Masters own the biosphere; we are contaminants. 
Pamela Silver: attempted to make photosynethic fish. "The interesitng thing is that nothing happened" 
RT  Preach it loud. Preach it proud! The Small Masters own the biosphere; we are contaminants.
Want! RT  Ooooo  RT Host manipulation by parasites new book oxford press 
"In conclusion... there really aren't any." Nice. 
 Pamela Silver wants custom RNA scaffolds to combine RNA binding hydrogenase & ferredoxin, and increase production.
Delwart: are bocaviruses pathogens? Boca1 seems to be. Some respiratory, some enteric 
 Preach it loud. Preach it proud! The Small Masters own the biosphere; we are contaminants. 
Jean-Michel Claverie: Megavirus chilensis now largest known virus; 200 nm, genome 1.25 megabases, natural host not yet identifed
  using RNA scaffolds to localize proteins in cells, broken GFP is fixed, will put hydrogenases together
Delwart: human bocavirus first discovered in 2005. Boca2 and more found by deep sequencing & consensus pcr. 
Brains of ants can be kept alive ex vivo for weeks! 
 2% of healthy blood donors in LA are parvo 4 positive. Jury is still out as to whether this is a pathogen, but it is endemic.
Jansson  - C. difficle therapy via fecal transplantation = restored healthy gut microbiome, resolved microbiome perturbation
Chk out  Sensititre AIM , doses most 96-well plates, eliminating skipped & repeats 
Delwart: unsure if Parv4 is a pathogen. A few studies suggest it might be but mostly anecdotal. 
I love the story of fecal transplants treating chronic diarrhea. Does that make me weird? 
Heidi Kong: Skin studies: increase in Staphyl species = decrease in microbial diversity = increase in skin disease dermatitis. 
Fungi get in between muscle cells of ants, lower mitochondria, atrophy muscle, ants bite leaves cant let go 
Janet Jansson discussing fecal transplants for Cdiff infections at
Baltrus: Costs of HGT may b widespread. Imp 2 better understand of evol dynamics; harness costs to treat bact infect 
Delwart: Parv4 mainly in blood exposed subjects (hemophiliacs, IV drug users)--blood transmitted? 
Crones disease shows dramatic decrease in specific beneficial (buterate producing) bacteria - Jansson 
Delwart: first ID of parv4 in plasma from regular sequencing; related to B19 and human bocavirus 
Just did a career mentoring breakfast at  and met some very bright students interested in gov't microbiology careers
 I am guilty of having dismissed parvo B19 as a "right of passage". It can be serious in preg women & sickle cell kids though.
Janet Jansson  - antibiotic therapy has long term impact on gut microbes
Delwart: parvoviruses: small viruses, environmentally resistant, common in cats, dogs, pigs 
Giant mimi virus = "food poisoning" for amoeba. Particles big enough to eat & "taste like bacteria bc of hairy stuff on outside" 
 I am never thinking of ants in the same way again ....evuh!
JJ: After antibiotic treatment, one clone of Bacteroides dominates gut for prolonged time 
Delwart: "nonstop shedding" of enteric viruses in healthy infants, sometimes for long periods of time 
 Janet Jansson on huge, prolonged impact of antibiotic treatment on gut  - up to > 2 yrs for some species.
3-5.30pm... MT : Room 305 is huge. Hope enough people show up so it looks ok(wd hate to see just 1st row occupied).
Award winner Stuart Levy’s lecture,“Predicting the Future for Antibiotics” is in the Esplanade Ballroom 306 at 10:15 am PT ^lg
Reading all the exciting  tweets is ruining my relaxing holiday… 
Check out poster 2417- Impact of Antibiotics on the Protein Profile of Outer Membrane Vesicles by Resistant Strains of Klebsiella
 judging from the level of clapping I am hearing from the adjacent room they are doing okay in there. 
  talking about carboxysomes in Cyanobacteria, cool microscopy to track them, screen for cells with more compartments
 Michelle Chang: block competing pathways, change reaction, inrease activation energy of reverse step: higher yield of biofuel.
Dare I say, David Hughes is nailing his zombie ant talk. Cool/gross videos? Check. Humor? Check. Complexity of behavior? Check.
Room 305 is huge. Hope enough people show up so it looks okay (would hate to see just the first row occupied). 
Delwart: 23 of 87 known viral families infect humans 
Manipulation of ant by fungus very specific: ant species, leaf position, time of day that ants bite 
 87 viral families and 23 of them affect humans.
Visit our poster (2614) at  - Detection of microbial DNA in spiked drinking water samples by nanofluidic qPCR, Starts in 60 min.
agreed! Hated to leave to head for parvovirus“: Mind is completely blown after just 2 minutes of the zombie ants talk.
AK: skin disease correlates with bacterial diversity 
David Hughes, Penn State opens talk on zombie ants w/ref to parasitic hairworms and cricket suicide 
Roossinck: persistent viruses can move from 1 kingdom to another (eg frm fungi to plants). Talk about getting around biologically!
ASM Live Broadcast 11:30 PDT. Ask questions about the human microbiome project via twitter tag   via
Worms make crickets commit suicide, wasps make caterpillars guard their cocoons 
Heidi Kong discusses skin microbiome and dermatitis 
Next up, Eric Delwart on emerging parvoviruses 
Host manipulation by parasites new book oxford press 
 videos of parasitic worm manipulating crickets already made the jump worth it 
Mind is completely blown after just 2 minutes of the zombie ants talk.
Roossinck: Panic grass survives heat bc of fungal infection, but fungi only protects if hosting persistent virus 
Questions about human microbiome project? Ask via twitter tag during ASM Live at 11:30 PDT today.
 M. Chang: using elongase enzymes from Trypanosomes to make biodiesels (C8 and C10) in yeast
Roossinck: plant adaptation to extreme thermal environments via viruses. 
Met with microbiologists, bioMerieux at . Good folks. Clear health pros can't depend on DC to fix system, must do on own.
Rasko: in EHEC, treatment w/antibiotics => upregulate shiga toxin (bad). See the same with O104--responsible for increased deaths?
Tweets from animal behavior session motivated me to jump here
Heijtx: question - GF mice less able to get nutrients from food. Is increased activity simply hunger? A: controlling for, still see
Aha! RT : RT : Ross says the bottled water she has surveyed has 100x as many microbes as tap water 
At   thanks Ye HMP for providing data to "biological analysis vultures" like himself to scavenge the carcasses of data
Rasko: core chromosomes conserved in all O104 isolates, but variation in plasmids 
Hey  attendees. 50% off our new high sensitivity chemi kits! 
Erwinia protein destroys tons of RNA from phage. -George Salmond
Heijtz: questioner: mother's milk factors stim neuro development and bacterial growth. Control for this? A: not sure how to yet. 
Roossinck: viruses transferred between fungal tissue and plant tissue 
Gut microbiomes can influence brain development in germ free mice, increased txtion of genes associated with some brain disorders. 
TS: multiple data types important when validating novelty- & more novel orgs at low abundance are awaiting discovery 
 Sharpton - HMP discovery of new bacteria Barnsiella that is as abundant in gut as E coli
Rasko: In banks other O104 isolates ID'd from Africa 
Heitjz: transcriptional profiling. >=2X fold changes in genes associated with neuro devel. disorder (schizophrenia). Following up
TS: Barnesiela is a novel genus as abundant in the healthy human gut as E coli 
GS: Erwinia Type 3 toxin is trimer of ToxN protein held together by toxI RNA 
TS: most abundant novel OTU looks like a Barnesiella species.
 M. Chang: reversibility in engineered pathways is important to consider - NADH cofactors are better than FAD, harder to reverse
Rasko: wiki & blog put together by groups around world with data, crowdsourced and edited/analyzed 
Rochellys Heijtz: how does gut microbiome affect mammal behavior, brain development? Yes-germ free mice more social, phys. active
Nice followup by Tom Sharpton on verifying novel taxa RT  Ashlee Earl: 61% of  OTUs low similarity to seq'd bacteria.
In germ free mice, autism/schizophrenia genes differentially regulated. Do we need microbes to develop 'normal' brains?
Rasko: test case for how genomics could be integrated into public health system. Sequenced in hrs, tests developed in days 
It would appear that everyone is in to the zombie ants. We just had a "day of the dead" influx into the room... 
TS: so who are the novel bugs? Firmicutes, Bacteriodetes mostly.
 Tom Sharpton - But maybe computational subtraction of novel junctions in rrn sequences will miss real rearrangements.
Looks like zombie ant lecture will be full 
TS: Putative novel 16s seq diversity can be overestimated without validation 
TS: without the validation process, novelty is vastly overestimated.
Except Archaea RT: : Bacteria "invented everything...." Eugene Rosenberg, Tel Aviv U 
GS: phage protection through toxin-antitoxin system 
Rasko: genomic sequencing showed mix of EHEC & EAEC characteristics. "hybrid" 
Roossinck: persistent viruses account for > 50% viruses in crop plants; understudied bc not causing disease; vertically transmitted
Heijtz: microbiota in 3rd trimester of mothers may be critical period for setting up neuro behaviors in progeny (at least in mice)
Rasko: Progression to HUS in other EHEC outbreaks 1-16%. Germany 24%, 50 deaths. Also unusual. 
TS: multiple sequencing platforms used to mutually assess quality of reads 
TS: mining 454 sequences for novel 16S, validating with pool of mapped illumina reads 
 What are top 10 most important/interesting/surprising lessons from Human Microbiome Project?
George Salmond: cryptic Erwinia plasmid protects against infection by numerous phage 
 Bushman: reverse trancriptase in gut bacteriophage associated with hypervariable phage head proteins
Rasko: EHEC usually hits kids; 2011 sproutbreak mostly people 20-40 years, women more than men, unique epi 
Tom Sharpton: HMP used Illumina & 454 sequencing to sequence gut microbiota 
 M. Chang: n-butanol as a gasoline replacement in the next ~10 years.
 cryptic plasmid in Erwinia contains toxin protein that might lead to phage infection protection through altruistic cell death
Rasko: so question, why did O104 cause so many cases when prev EAEC stx+ strains didn't? 
Marilyn Roossinck: persistent aka chronic viruses in plants & fungi; mostly RNA viruses 
Rasko: A few previous reports in lit of EAEC producing shiga toxin (hallmark of EHEC) but seems rare, small # of cases 
just reminding people that  is tweeting all kinds of cool disease-geekness from 
Very valuable RT : Re  I have compiled the recent human  papers & news stories here
Tour  in a restored VW Bugster Experience convertible! Show  badge for a discount
Heijtz: Germ free mice have different turnover of serotonin & dopamine; also changes in anxiety related gene expression
Check out our fluid handling broad flow performance range at booth 1015  
Rasko: EAEC described in 1983, contains large virulence plasmid in most isolates--diverse and "different flavors" of EAEC 
Rasko: EHEC (enterohemorrhagic E coli) & EAEC (enteroaggregative E coli) --O104 is a merger of the two types, backbone EAEC
 Bushman many DNA phages with hypervariable region associated with reverse transcriptase (like Bordetella phage / J Miller's lab
Heijtz: germ free mice also seem to have lower anxiety, and higher sociability 
! RT  What if viruses invented bacteria for the same reason? 
 Hasty: circuits from E. coli seem to work Salmonella pretty well, plan to use it to seek and destroy tumors in mice.
Final day of Clinical Microbiology Mentoring sessions! Stop by the Clin Micro Lounge (room 112) at 11am and 1pm today! ^tg
Heijtz: germ free mice display increased spontaneous motor activity
 poster 2632 Paul Thomas on PortEco: A Portal for E. coli resources. Also at booth 1623 next to 
 jumping! Out of the microbiome and into microbes affecting animal behavior.
RB: comparing viral metagenomic functions to bacteria reveals their parasitic nature. 
Totally appreciating all the  tweets. I'm sad that I'm not there!!
 See Ron Caspi from BioCyc at poster 2627 : learn about the 2000 pathway genome databases in the collection
RB enriches for viral particles and then sequences to study our gut virome 
Fair: Transmission of virus? Postulates tick vector; no livestock or wild animal die-offs in area to suggest epidemic there 
Heijtz: children along autism spectrum have correlative GI problems & abnormal microbiota 
RT : Your microbiome may be influencing YOUR mating behavior. didn't think your bugs could control you like that, did you?
 Rick Bushman on gut virome: 10^10 viruses per gram!
Does the abnormal gut flora attribute to the symptoms of autism?
RT : FB: 10^10 - 10^11 viruses per gram of human stool. Overwhelmingly phage. 
Heijtz: microbial pathogen infects can increase risk for neurodevelopment disorders 
Peggy Cotter on competition systems in Burkholderia... these genes control biofilm formation too. So cool. 
 R Bushman - Human gut virome >10^10 viruses (most phage) per gral feces
Fair: Not able to grow virus, but found patient & contacts had antibodies against viral protein 
Rochellys Diaz Heijtz: impact of commensal gut microbiota on brain development 
 J. Hasty: 'biopixels'! Wired to sense arsenic. Future application: handheld biosensor made for very little $
FB: 10^10 - 10^11 viruses per gram of human stool. Overwhelmingly phage. 
Fair: sample sent to US for deep sequencing. Sequence from novel rhabdovirus discovered, dubbed Mangala virus. 
 Hasty: H2O2 gas is produced in bursts by GFP, quorum sensing in a colony, but redox between colonies in their micro fluidics device
Frederic Bushman, dynamics of the human gut virome 
Some with <70% 16S sequence identity! MT  Ashlee Earl: 61% of  organisms low similarity to sequenced bacteria.
Rick Bushman talks about dynamics of human gut virome 
Your microbiome may be influencing YOUR mating behavior. didn't think your bugs could control you like that, did you? 
Bacteria "invented everything; they probably invented animals and plants as a place to hang out." Eugene Rosenberg, Tel Aviv U
Fair: 2 patients died, no samples taken. 3rd patient had samples taken. No epi connection btwn patients but lived in same village
 J. Hasty showing some mind blowing microscopy movies of their synchronized oscillators. Synchronized colonies flashing! How??
 Ashlee Earl: 61% of  organisms (OTUs) have low similarity (<98% 16S Id.) to sequenced human associated bacteria.
RT : Bacteria in gut of flies influenced mating choice!
Fair: Outbreak 2009, 3 cases of hemorrhagic fever in DRC; tested for known HF viruses but were negative 
It’s poster day at ASM! Stop by poster 2294 at 10:45 am and 2708 & 2867 at 1 pm. It’s some good stuff, trust me. 
With all these human microbiome talks, we're going to see probiotics for more than just digestive health sooner than later... 
Rosenberg: need to change how we teach biology; it's not possible to understand 'higher' organisms without considering microbiota
 J. Hasty: networks exploit queuing as a form of signaling because it's fast - cool! Wow these oscillators are awesome.
Fair: Before their study, arenaviruses, bunyaviruses, flaviviruses, filoviruses only groups known to cause hemorrhagic fever 
RT : Villareal: the world is and always has been viral; horizontal gene transfer by viruses 'edits' tree of life 
Trying to see 's talk but the overflow but he's so popular I have to go to the overflow room 
Bacteria in gut of flies influenced mating choice! 
 HMP so far: 200 healthy volunteers, 18 body habitats and generated over 1,000 reference genomes.
Villareal: the world is and always has been viral; horizontal gene transfer by viruses 'edits' tree of life 
AE et al are generating genomes from these taxa 
 yes! Classic Pac-man as ClpXP! Hasty is an old school gamer for sure. This protease can get overloaded an alter behavior of system
 I bet I'm not the only senior academic to have just googled hypothesis vs theory, just to be sure! 
Fair: examining hunters in Africa to look or novel viruses. Lots of exposure to blood/body fluids etc 
Explain differences between people? RT : Interesting thought: are our commensal microbes helping I keep us warm?
AE: The HMP generated genomes recruit most of the microbiome data, but some reads don't have a nearby genomic home 
 Thinking of viruses as a data cloud that surrounds tree of life
How is the ref collection so far? Relatively large amount of seq data can't be mapped to current ref set 
Next up, Joseph Fair talking about a new rhabdovirus causing hemorrhagic fever in Africa 
 really like that J. Hasty has an appreciation for the classic work done in SynBio way, way before it was called SynBio
If scientists dont use hypothesis vs theory correctly, how can we expect it of general public?  
Villareal: viruses are natural editors of host code 
 J. Hasty: Assume basic biochemical reactions correct, but relax assumptions about timescales / rates
 impressive numbers. HMP has contributed over 400 unique bacterial species
Wow. The JMP has sequenced most of the available human associated microbial genomes 
 Ashlee Earl - importance of reference genomes to interpret metagenomes; >4000 bacterial genomes sequenced
Biology / disease fans (well, u know what I mean) :  is tweeting from  -- Take a look at 
 Ashlee Earl & the most wanted bacteria from the human microbiome HMP has characterized microbial communities in 18 body habitats.
Virus phylogeny makes a "ghost image" of tree of life 
 J. Hasty: some weird biology thing happens down there, let's not worry about it - trying to reconcile model with actual data
Rosenberg... hologenome as unit of selection. Yet another "layer" to evolution. 
 Charles Chiu using  for deep sequencing of clinical microbiology samples in <24 hrs Multiplexing saves money and time!
Create complete reference for hmp. 100 most wanted for ref collection. 
AE discusses the importance of generating reference genomes to understand community function 
Rosenberg argues that social behavior like kissing and hugging has evolved to promote transfer of beneficial microbes 
Congrats to 's gift card winners: Greta Rijal, Dolorsas Tseng, Chris Minion, Fabio Amoral. Wanna win too? Visit booth 1625.
Adding, I've met a lot of wonderful people in science so I consider that very high praise. 
Ashlee Earl discusses the most wanted bacteria in the human Microbiome 
Chiu: handlers of baboons (had outbreak of AdV) developed antibodies to primate adenovirus 
Be sure to stop by bioMérieux booth 1301 at  for a personal tour through The Microbiology Pathway!
 Huttentower - Crones Disease, difficult to point to specific microbe change but clear metabolic changes predicted
Luis Villareal: viral ancestors to antiviral systems; starts w lytic & lysogenic phages 
The Japanese: can digest agar due to Hologenome, too bad many of us can't digest ethanol. 
Next up Ashlee Earl. One of the nicest people I've ever met in science! 
Ashlee Earl (Broad Inst): The "most wanted" bacteria from human microbiome.  
Missed most of this talk on zoonotic adenoviruses but it's fascinating
One cool thing about hmp project- massively collaborative
Rosenberg: HGT between bacteria found on sushi & Japanese population endowed this ethnic group with the ability to digest agar
Not sure why Rosenberg's holobiont theory only applies to plants and animals 
RT : Rosenberg: coral bleaching was shown to be caused by Vibrio shiloi infection (satisfying Koch's postulates)
Interesting thought: are our commensal microbes helping I keep us warm? Talk by Eugene Rosenberg 
Works well on assembled metagenome too? RT  H: uses metaphlan to characterize taxonomic composition of metagenomic data 
 Huttenhower - metabolic predictions from metagenome very different from metatranscriptome
More Microbial Genomes at : The second full day was chock-full of 'omics-related research 
microbiology tweeps  anyone got experience using iphone style apps for counting colonies or this type of app for Mac's/PCs please?
I welcome our viral overlords and readily acknowledge their genetic and numerical supremacy 
 J. Hasty 'I guess I'm getting old if I've inspired new scientists'
CH identifies some functional differences between body sites
Viruses have infiltrated the presentation system, causing technical difficulties in virus session 
CH: uses metaphlan to characterize taxonomic composition of metagenomic data 
Rosenberg: recovery of corals could be due to an epidemic of beneficial bacteria that kill V. shiloi 
Rosenberg: coral bleaching was shown to be caused by Vibrio shiloi infection (satisfying Koch's postulates) 
RT : Suttle: no viruses = lose most genetic and biological diversity on earth 
Tech is simple; driving it so well during a talk is hard++. RT Graphical display tech to show these data is stunning 
Huttenhower: linking microbiome to function. . Live now on ^gm
Last day of  don't miss the Idaho Tech booth 514! Check out the FilmArray and grab the next bug magnet for your collection!
: girus CroV attacks zooplank, which responds with transposon-like Mavirus to attack CroV 
Curtis Huttenhower talks about species, strains, and functional roles in microbiome 
Curtis Huttenhower (Harvard): Identifying species, strains, and functional roles in the microbiome  
 Some really nice on-the-fly exploration of multidimensional data during Rob Knight's  talk using KiNG
At hmp update session. Graphical display tech to show these data is stunning 
 infant gut microbiota changes radically. I'm curious as to some of the subtle drivers of these changes that may be manipulated.
Talk by Kevin Theis: hyena scent gland bacteria are group specific and contribute to scent variations in each group. 
Suttle: CroV = giant virus that is parasitized by smaller virus (related to transposons) 
Marine viruses enhance phytoplankton growth by freeing up ammonia via lysis of bacterial cells. Driver of global chemical cycles
 7 families ssDNA viruses known, ~125 more out there
RK wows us with a beautiful visualization of how infant microbiota changes to adult over time. 
Vast majority of marine viruses not yet known (no known homologs)
Mogous: look for type 6 effectors with heuristic search for characteristics lik # presence of immunity proten neighboring effector 
 Rob Knight: no evidence for "enterotype" clustering in Human Microbiome healthy cohort  
Viruses essential to nitrogen cycling: with viruses more ammonia better phytoplankton growth 
RK: discusses whether enterotypes exist in the human gut. Sees gradient of types, not clusters. 
 Erik Wommack and Shannon Williamson are the only ones I know doing deep sea virus work 
Watch the complimentary live stream of the Human Microbiome Project session from  now!  ^gm
Phytoplankton (Synechococcus) grow faster when viruses are present 
Theis: bacterial community composition of hyena scent pouches vary between clans, supporting group-specific chemical recognition
Suttle: took 20 yrs to publish bc he didn't believe it - viruses enhance phytoplankton growth rate 
Major goal is to integrate healthy microbiome data with more diverse host samples 
 L. You: iPAD intermediate programmed altruistic death synthetic lysis circuit. I bet Apple's lawyers come calling!
 latest news from human microbiome project check it out live online now!”
  5.2 million non-redundant gene reads through the Human Microbiome Project.
RK: microbiome taxonomic diversity changes across the body and across patients 
 Let me know if anyone says anything about *deep-sea* viruses at  - think that's an unexplored frontier
Theis: 16S survey of hyena butter reveal 78 genera composed of Firmicutes, Acintobacteria, Bacteroidetes that produce SCFAs
 Suttle: all ocean viruses laid end to end would span 10^7 light years in length!
Why care re:viruses? They lyse 20% of ocean biomass per day
Rob Knight discusses HMP healthy cohort microbiome characterization study 
Suttle: 10 ^30 viruses in the ocean. Says number makes national deficit look trivial 
 If you swallow a mouthful of seawater, swallow more viruses than people in USA 
Suttle: 10 million viruses per drop of seawater. So when you swim you swallow more viruses than people in the United States
By the numbers, viruses dominate in the ocean 
Theis: scent marking by hyenas is known as "hyena butter"
 latest news from human microbiome project check it out live online now!
 Curtis Suttle: 95-97% of ocean biomass is microbial.
Suttle: 90% by weight of living organisms in the ocean are microbial. Marine viral carbon ~ = 75 million blue whales 
 Weight of viruses in ocean = 75 million blue whales
P aeruginosa Tse1 cleaves only crosslinked peptitiglycan. Only cleaves donor peptide strand 
Theis: if fermentative hypothesis is true, animal scent glands should harbor odor-producing bacteria 
Good reminder: for billions of years the biosphere was bacteria, archaea, and viruses 
Morning,  tweeps. I have a power strip on the right side of 305. Plenty of room.
Suttle: no viruses = lose most genetic and biological diversity on earth 
Lita Proctor introduces the Human Microbiome Project (HMP)
Mogous: type 6 effectors target bacterial cell walls 
Lita Proctor (NIH): A brief overview of the human microbiome project 
Theis: scent making odors are products of bacterial metabolism (=fermentation hypothesis) 
Curtis Suttle: viruses in the sea, drivers of global processes and pool of unrealized diversity 
How Microbes Affect Animal Behavior: Kevin Theis 
Up first on the last day: Joseph Mogous. Type 6 effectors 
Using NGS in public health, more microbiomes, a talk by Mike O, I'm very excited for this last day! 
At  waiting for session "imagine a world without viruses". I don't want to...what would I teach in the fall if no virology course?
  I hope you will tweet highlights. I was planning to go but the siren song of the microbiome pulled me away!
  tonight I will be sleeping upright in the Virgin America flight back to Fort Lauderdale. I am beginning a long 24hrs!
Starting day by going back to my scientific roots; in the "Imagine a world without viruses" session 
one nice thing about the Moscone center: dance music in the bathroom 
Thank you ASM for a great meeting in San Francisco 
 I am in 305 and am very curious about all the Human Microbiome Project info that is about to unfold. Very curious indeed.
Special Issue Mycopathologia on Cryptococcus and Cryptococcosis. Free Access until 30 June  
Last day of ! Only a few more days until I'm back in SoCal
 this certainly gets the day off to an unusual start. A parade to Moscone w/ 50+ microbiologists from our abandoned bus!
 no one hurt in our shuttle bus accident but the sideswiped BMW driver is being a prick. We all had to abandon bus & walk 2 Moscone.
It's the last day of  ! Don't miss talking to DNASTAR's Katie and Stephanie at booth 1518 from 10:45 - 2:45.
 not one of my sites, but I lurk: . Forum & wiki re:sequencing and seq informatics.
 whoops our shuttle bus just sideswiped a 6 series BMW. The BMW driver was driving like a jerk! No one hurt, just a good karma smack
 6/19, 3:00 PM Esplanade Ballroom 306 - On Top of Outbreaks: Rapid NGS Deployed for Pub Health & Clinical Micro
Can you find a mini- at ? They could be anywhere at the Moscone Ctr starting at 10:45 this AM!
Getting set to put up my poster for the day. Board 3057. Great meeting. Very tired but very excited. 
Today is showtime for me! AV not perfect, but will put on my Microbial Proselytizer clothes & preach it...to the microbial choir!
Could ASM sponsor a BIG YEAR competition? Instead of most birds discovered, most microbes! Winner crowned at 
Meet our blue-footed booby at  booth 723 and ask for a sample of RE-Mix restriction enzyme master mixes
Our editor Charlotte Sage is at  for the last day of the conference. Need her info?  
Our editor Charlotte Sage is at  for the last day of the conference. Need her info?  
Looking forward to zombie ants today. I've seen a short version of this talk and it's fascinating! 
Final Day in San Francisco at ASM Annual Meeting,come visit MIDI at booth number 1036. 
 Really wish I was at this conference. Being kept updated via twitter! 
Heading over to  early to get set up for my talk later this morning. Should be a fun last day!
Q: Why did I come to ? A: For talks like Rob Knight's (U. Colorado).
The Tree of Life: Quick summary of session at  on “The Great Indoors”  
Thanks for all the tweeting from . I feel like I am there. Makes me feel bad that I'm not there. Why am I not there?
 tonight (8 pm bst) should be interesting, I wonder how many people from  will be there...
 everyone's at  except me who's in France sampling the foie gras microbiome
Wow! RT “: Pete Greenberg, DC White awardee lecture: research of Diggle's group being shown much respect here.
SPACER



Dad I am raising a glass in your honor from a Cambridge bar called Miracle of Science 
Autocorrect is getting a workout on a lot of phones at . iPhones don't know many microbes...
 B. Zhu. w/o 5.5 host tRNAs can serve as primers for DNA replication. But this doesn't explain what 5.5 wt does.
Crazy! RT : RT : Lu Zhang: 28.9 million pounds of antibiotics are consumed by animals in the US annually
Congrats to my dad Prof Myron Levine for winning prestigious Hilleman award 4  research 
 Enjoying ASM 2,680.3 km away from the venue at the comfort of my bedroom pushing sleeping son. Am I eco-friendly or poor? ;)
that is just crazy!: “: Lu Zhang: 28.9 million pounds of antibiotics are consumed by animals in the US annually 
RT : EC: Sampling infant dorsal tongue, retroauricular crease, and feces = "drool, dandruff, and dirty diaper microbiome"
 B. Zhu. T7 5.5 mutants suppress primase deficiency. Suppressors include deletions of 5.5 (which interacts with HNS)
Dictyostelium discoideum has agriculture, meets conditions of domestication, prudence, defense, across generation transmission
Did you catch that last slide of syntrophic electron transfer? Geobacter literally taking crap from Shewanella. 
Lu Zhang: 28.9 million pounds of antibiotics are consumed by animals in the US annually 
Change kin specificity by allelic replacement. Props for nice expt design! 
 collette Fitzgerald has me convinced Campylobacter is going to make Salomonella seem tame. Guillain- Barre Syndrome assoc w/ Campy!
Found high levels in TraA in soil isolates and kin groups have similar sequences in Myxococcus. Green beard Darshan Pathak
 RT : Indoors, we shed about 10 million bacteria per person per hour, according to Preccia 
 B. Zhu Phage proteins represent the greatest reservoir of protein diversity.
Looked at environmental isolates and found clear kin recognition groups according to this gene in Myxococcus xanthus Darshan Pathak 
Kin recognition. Reminds me of proteus swarm recognition factors
Vaginal baby lots of bacteroides from birth, two c-section babies bacteroides dont develop until 1 year 
 Next: Bin Zhu on T7 5.5, hns, and tRNAs
Plos genetics 2012 8:e1002626 TraA and B determine transfer
Gene for Myxococcus kin recognition is on cell surface, adhesion, over express and over stick. interesting gene. Darshan Pathak
Indoors, we shed about 10 million bacteria per person per hour, according to Preccia 
Use cherry and GFP to look at kin recognition in Myxococcus genes are TraA and TraB genes Darshan Pathak 
Question, could this be common in biofilms? 
  just like sprouts, I gave up my contact lenses several years ago as well. 
Cool construct w mcherry in outer membrane, gfp in cytoplasm. Fusion and transfer of om components in biofilms! 
Nice reporter called Cherry on membrane, and GFP inside other cell, shows membrane fusion between diff. cells. Darshan Pathak
  Contact lenses interrupt the sheet of tears that normally prevent microbes on eye surface 
Next myxobacteria. They are awesome. Lots of crazy multicellularity. Fruiting bodies, predation. Thanks Marty Dworkin! 
 True! Wondering if anybody ever studied the commensal viruses in, on or around us  
Myxobacteria social and cooperative, build huge fruiting bodies, social whole life, group predation, group fruit. Darshan Pathak
EC: Sampling infant dorsal tongue swab, retroauricular crease swab, and feces = "drool, danruff, and dirty diaper microbiome" 
 N Shechter: Condensation after DSBs leads to pairing of homologous sites. Coalescence is RecA-indep. Merging needs RecA
Now take away fumarate use gold electrode. Measure current on electrode only from coculture! Very cool 
Cool tool, on the elctrode, but what is it telling us? Co-culture, make dependent, but what then? Aunica Kane 
Elizabeth Costello investigating post-natal development of oral, gut, and skin microbiomes 
  oh God! Thanks for planting that in my head. "microbes in contact lenses cases". 
 Shewanella and Geobacter co-cultures, cats and dogs playing together! It's cool, but I'm quite biased!
 24 species of Campylobacter. Who let this happen? I think there were 3 when I was an undergraduate. And the number is growing.
Make shewanella unable to respire w fumarate for TEA. Obligate coculture -manufactured syntrophy! 
 Stuart West is the clearest thinker around on social evolution, or at least in the top 3. Check out his papers. 
 I bet there are some interesting microbes in worn contact lens cases! 
Campylobacter up now - personally my favourite foodborne illness causing bacteria 
 N. Schechter: DSBs lead to DNA condensation... are DNAs aligned during condensation? Look with tetO arrays
Brown: FDA used metagenomics, found Salmonella-killing bacteria on CA tomatoes; now using bacteria to control Salmonella on VA toms 
Shewanella uses glycerol, geobacter cannot. Geobacter uses acetate from shewanella. Commensalism 
Now providing the sample of my worn socks for the study of microbial diversity and ecology ;)  anybody interested?
Laser pointers are so distracting. We can read better if they don't stir the slides with their annoying dot. Aunica Kane 
 Eric Brown gives me hope I may actually be able to enjoy a salad without fear again!
How often are the systems with great engineering also collaborating with clear theoreticians? Aunica Kane 
QS/DS tweets really sparking my curiosity. Need to investigate this controversy. 
 Nelia Shechter: segregation of sister chromatids creates a problem for DSB repair. Homologous sequence not physically close enough
 still metagenomics is a fascinating area of study for product safety. I bet we see a lot more and soon at that. 
Shewanella & Geobactor co culture one benefits, one neutral, both gain, or both gain plus on a surface Aunica Kane 
Eric Brown: 'Salmonella outbreaks associated with tomatoes on east coast USA but not west coast'. Amazing. 
Eric brown- culture methods will always be important 
"Culturing methods are always going to be important" 
Engineered cocultures commensal, obligate, obligate on electrode
Gralnick lab, co culture Shewanella and Geobacter engineering. Track electrons moving across cell membrane to see coop Aunica Kane 
Extracellular electron transport! Bioreactor with an electrode, measure current. Cooperation? 
RT : QS important to keep expensive things to be released only when others around. Stuart West 
 Eric Brown CA tomatoes have a lot of naturally occurring gram + that inhibit salmonella. Not happening on eat coast tomatoes.
  As I understand, it's well-studied in cystic fibrosis. Tricky to sample 
  Joerg Graf presented miseq data using new chemistry (2X250bp reads) for comparative genomics of A veronii strains.
Award winner Bernhard Palsson’s lecture “Microbial Metabolic Systems Biology” is in the Esplanade Ballroom 310 at 5 pm PT ^lg
Aunica Kane from  lab!  coculture of shewanella and geobacter! 
Brown: FDA moving into metagenomics but diversity of microbial cmnities on produce is big challenge to food safety investigations
Interesting Q: could 3rd trimester microbiota be beneficial shift to inoculate newborns so they have rapid growth? 
Would like a microbial pathogenesis of the pulmonary environment book please!  
Question came up: how do we know if some thing is an ortholog? Pretty tricky I think without detailed analysis. 
Personal vs. group benefits misleading bec. of relatedness, QS and DS not different, question is it social? need empiri Stuart West
 Eric Brown: the cereal outbreak traced to Salmonella Agona was embedded in the cement mortar and active for 10 years. Adaptation!
More quest, diffusion rate matter? freq depend? Cheaters in nature? exploit fr medical intervention, but is it natural?Stuart West
Great changes in gut microbiota in children 0-2. Similar rates across cultures 
Manipulate cell density & quorum sensing independently for group size role. There is a bigger effect at high density Stuart West
Citti: the more we sequence, the more we learn. Pangenome studies change paradigms 
 wait a Salmonella Agona outbreak in dry cereals in 1998 & 2008. Somehow I missed this one. Data 2 be published soon says Eric Brown
Second most fundamental question is whether group size matters for producing exofactors, hardly tested. Darch 2012 PNAS Stuart West
Transplant gut microbes from 3rd trimester women to mice, mice get fat 
 the e in eDNA is not like the e in eBook
Social trait QS DS only tested in 2 species Pseudomonas aeruginosa, Streptococcus pneumoniae, should be natural envts. Stuart West 
Rob Knight now discussing changes in gut microbial community composition between pregnancy trimesters 1 and 3 
Third trimester women have unique gut microbiota 
Christine Citti: ICE ICE baby! A new integrative conjugal element in mycoplasmas may save it from evolutionary dead end. 
3rd trimester pregnant women have microbiome unlike any other- Rob Knight DUH. They have constipation like no others too. 
How important it is to think carefully about the right question, do careful experiments, borrowing from other fields Stuart West
Using gut microbiota sequence scientists can predict with 90% accuracy whether person is lean or obese 
FDA's Brown: whole-genome sequencing is "elephant gun" in characterizing food-borne outbreaks; has some uses but still "pricey" 
True big question is about whether a trait is social, exper. is do, don't grow separately, grow together, Pseud aerugin Stuart West
CC: large chromosomal regions can get acquired, up to 90kb, in some cases without the ICE 
Please, empiricists, don't try to do experiments between diffusion and quorum sensing, because it won't work. too simil Stuart West
QS social, needs >2 cells, diffusion, direct benefits, small groups benefit both quorum and diffusion, so these similar Stuart West
QS is a social trait, so how it impacts relatives matters. With diffusion shares a lot of benefits with quorum sensing Stuart West 
RT : Wachsmuth: reminder, worldwide 1.8M children /year die of diarrheal disease, many of them food/water borne
CC: how is HGT happening? Conjugation by integrative conjugation element 
Comparing group and personal benefit is confusing, need to depend on genetic relatedness, big confusion in microbiology Stuart West
RT : Turtle apocalypse(!) - salmonella cases from pets
 all it took was Salmonella and a salad bar in an Oregon restaurant too make history as 1st documented US bio terror attack.
RT  I am with Besser on this. I no longer eat sprouts. Too many other things I enjoy more. Not worth the risk to me.
454sequencing at $12k = Sanger at 4 million -Rob Knight 
according to  sprouts keep popping up as the culprit in food outbreaks. Liz: avoid them
Hard to ask how much siderophore or elastace to produce on own bec. need to include kin. If rel is one, then no confli Stuart West
  Joerg Graf talking about using RNA-Seq to study the medicinal leech Hirudo verbana 
CC: two of these seven showed 18% of genes were horizontally transferred with another myco group. 
RT : Besser: always new vehicles for outbreaks: spinach, carrot juice, hazelnuts, peanut butter & more 2006-11 
mT : (2/2) Besser: but put in prevention measures to prevent future outbreaks 
mT : (1/2) Besser: reasons to detect outbreak: not only limit ongoing illness, but 
Hamilton's inclusive fitness is what is maximized, and it is the direct and indirect that matters. For bacteria? Stuart West 
 Eric Brown reminds us about the bio terroristic attack in US back in 1984 when the Bhagwan tried to take an election w/ Salmonella
RT : Besser: Of 10 largest outbreaks, 7 probably wouldn't have been detected w/o current surveillance techniques 
Inclusive fitness! Kin and group selection! More controversy
Sat with DC White's wife Sandy for award lecture - remarkable lady keeping alive his work. Note to husband, please do same for me.
RT  Besser: Every year 1 in 6 Americans (48M) get sick and 3000 die of foodborne illness $77B/year cost. 
CC: Looking at ruminant mycos specifically, still a low core- 201 genes, with 7 sequenced genomes down to 11 new genes per genome. 
QS not for good of group, DS not for self. Remember why organisms appear designed? Solved by Darwin. Remember kin sel. Stuart West
Evol theory gene dynamics lead to fitness max  fisher
Phylogenetic analysis of gut microbiota of different animals shows clear differences by diet (-vory) 
RT : CDC's Besser: sprout safety has improved but "I still don't eat sprouts" 
All these  tweets make me wish I were physically attending the meeting.
 It could have originated as diffusion sensing and then evolved a new role as quorum sensing. Why not both?
Are QS and diffusion really competing hypotheses? No,confusing, based on error of understanding nat sel. Stuart West 
Lots of people at the foodborne associated outbreak session - its nice to see this topic can still draw in a crowd 
Competing hypotheses? Or false dichotomy based on misconception about nat sel, confuses the issue  n.b. I think he's right
 Salmonella is still responsible for 11% of all our food borne outbreaks. It is an adaptable player!
Salmonella is not just chickens: reptiles, pistachios ... 
Analysis of Mycoplasma core/pan genome. Using 59 genomes.
Is Rosie Redfield right? Is QS all about self, just how fast the flow in envt is? Competing hypothesis Stuart West 
CC: 125 orthologous clusters shared among all, very large open pan-genome. 
Rosie Redfield suggested diffusion sensing rather than qs 
QS important to keep expensive things to be released only when others around. Stuart West 
Quorum sensing, bacteria release small molecules, then uptake results in increased production and other things released Stuart West 
 25 min before my student's talk in room 102 (4:30)! Geobacter and Shewanella working together! 
CDC's Besser: We're in baby chick "apocalypse;" backyard poultry-keeping leading to Salmonella infections linked to chick/duck eggs
Otto Cordero: concludes that yes, although mechanisms will of course differ from animals. Nice work, probably in Science this year
CC: 20 more coming. Three total phylogenetic groups represented.
Here are why questions, not how questions, evolved reasons, Tinbergen, black headed gull example, the brilliant Stuart West
Good to know: scientists who know food safety dont eat sprouts.
Why questions - survival value vs how questions 
 Courtney Naff: green snow and red snow (they exist) contain different eukaryotes, i.e. not young & mature snow algae.
Besser: field is moving away from culture & to genomics. 82% of GI disease from unknown cause, genomics can help ID 
Otto Cordero: can related but distinct bacteria act as socially cohesive populations given dispersal and horizontal gene transfer?
Culture independent methods will have an effect on outbreak investigations, typing methods need cultures 
 "deep sequencing stool". Pass the brain bleach please.
CC: Mycoplasmas have evolved through gene loss- very small low GC genomes. Originally no evidence for HGT 
Rob Knight is telling a great story about the study of the human microbiome 
Even "core" microbes vary by orders of magnitude in abundance in different people 
Giving mice subtherapeutic antibiotics or high fat diet perturbs microbiota and increases adiposity- Laura Cox 
Is competence an epiphenomenon? Signs point to "yes" no one wants to admit it?
 Besser: when it comes to food borne outbreaks, every outbreak is different.
MT  Invisible Residents -A diagram of the microbes that live inside of us, from my story in NYTimes 
Christine Citti is next on the pangenome of mycoplasmas 
" As Besser talks I am more and more impressed with PulseNet. Imagine where we would be in food safety without it."
First 4 weeks of life critical sensitivity for development of gut community 
Bacterial populations can act as ecological and social units, lots of diversity, variability in behavior Otto Cordero 
O.X. Cordero: cosmopolitan peptide from Vibrio ordalii... 'what the hell just happened?' 
 only salmonella can exploit the host production of IL-22. Other pathogens aren't affected. Nice work Dr Behnsen!
CDC's Besser: sprout safety has improved but "I still don't eat sprouts" 
 As Besser talks I am more and more impressed with PulseNet. Imagine where we would be in food safety without it.
JD: need to isolate more species to get more new products.
Low dose antibiotics (STAT) change gut microbiota in mice, cause weight gain. Cecal transplant exp show its the microbes. 
CDC's Besser: food-borne illness sickens 1 in 6 Americans @ cost of $77.7 bln/yr 
JD: big jump in new classes when you get past the species cutoff for Streptomyces 
Quorum sensing and chitin required for v. cholerae competence gene expression. Cool model for HGT in envt. 
Vibrio ordalii V. crassostrea V tasmaniensis killing molecules are in polyketide synthases, Otto Cordero 
Pete Greenberg, DC White awardee lecture: research of Diggle's group being shown much respect here. 
Vibrio ordalii, killing biased to other pops. within pop resistance. Killing phenotype variable, mult. substances used Otto Cordero
Cytidine represses chitin induced competence in CytR dependent manner  not sure this says both hgt and nucleoside met matter
 Besser we are in the midst of the turtle apocalypse. I must have had 50 of those little green guys as a kid. They are back!
Mechanisms for antagonism, one pop, inc. resol, look at phenotypes to get at genetic mechanisms. still bacteriocins Otto Cordero
JD: clustering genes to try and pull out unique classes of antibiotic production genes from 231 genomes 
Building rarefaction curves with gene clusters. Extremely steep!
Besser: "turtle apocalypse" --illegal but can buy them on net. Baby chicks, reptiles, feeder mice, petting zoos assoc w outbreaks
Turtle apocalypse(!) - salmonella cases from pets 
There is an interaction threshold distance matches the antagonism boundary also. Pops not clonal, so more interesting. Otto Cordero
Discussing finding NRPS gene clusters across 231 genomes. I like talks with large data sets. 
Model repressing a repressor to activate with CytR. Crp epistasis supports model 
Holy crap. MT   public restroom surfaces heavily contaminated w hardy bacteria, virus -reqd 20m of 10% bleach 2clean
 I am with Besser on this. I no longer eat sprouts. Too many other things I enjoy more. Not worth the risk to me.
Joerg Graf's talk on RNA-Seq for digestive tract microbiomes using is about to start in 10 min, room 130-131 
Vibrio killing network = looks like hair ball 
6 genes, then interact network, looks like a hairball Prob of killing inc. with certain distance conflict boundary Otto Cordero 
Besser: always new vehicles for outbreaks: spinach, carrot juice, hazelnuts, peanut butter & more 2006-11 
Nice talk in pan genome session on identifying functional gene clusters. 
Industries have had to change following pulse net triggered outbreaks- a great start to reduce foodborne illness 
CytR is a crp dependent anti activator, neg regulator in e. coli. Strong aa level conserv, func. complement 
Vibrio, look at interact in lab, killer and sensitive, 185 strains 35000 potential interactions hypoth network Otto Cordero 
JD: genomes can give a starting point for physiological tests to produce new products. 
Besser: reasons to detect outbreak: not only limit ongoing illness, but but in prevention measures to prevent future outbreaks 
So tiny! RT  Holding a 454 chip. Interesting to see where our sequence comes from. 
  Imp study w Eww factor: pblc restroom surfaces heavily contaminated w hardy bacteria, virus -reqd 20m of 10% bleach 2clean
Vibrio and pop structure from Plum island 1000 strains Hsp60 Hunt et al Science 2008 Otto Cordero 
Can bacteria have social population structure? Baas Becking's all everywhere but envt. selects. What about pop strctureOtto Cordero
Great stuff happening at . Wish I was there.
James Doroghazi U of Illinois, discussing ways to find novel antibiotics with more genome sequences 
RT : Besser: Of 10 largest outbreaks, 7 probably wouldn't have been detected w/o current surveillance techniques 
Old fashioned tpn insertion screen loss of luciferase 
   if we ever learned restaurants would not still be offering us the option of "pink" in our hamburgers.
MIchi Taga: corrinoid exchanged (same family of compounds as cobalamin = vitamin B12) among bacteria in polymicrobial populations 
Germ-free mice given microbes from ob/ob mice gain weight faster than those given microbes from wt mice 
Wow that's alot! RT  Besser: Every year 1 in 6 Americans (48M) get sick and 3000 die of foodborne illness $77B/year cost.
Grow biofilms on autoclaved crabshell transformation expt
Quick plug for any1 still around@  tom:3-5.30pm Rm305"Your Topics,Your Voice,Your Choice"Not that I'm biased in any way of course ;)
 the number of people who die from diarrheal diseases is between 2-5 million annually. Surely we can get a tighter data point here.
: Wachsmuth: Foodborne outbreaks 2010-12, sprouts major culprit. "We haven't learned." ” --do we ever? Sigh.
Chitin and qs induce dna uptake. Biosensor to test for autoinducer requirement 
RT : Besser: Every year 1 in 6 Americans (48M) get sick and 3000 die of foodborne illness. $77B/year cost. 
 cell death as a strategy. Salmonella needs to employ the goldilocks paradigm. Just the right amount of death.
Laura Cox - Early life microbiota shapes adult body composition 
What do you mean shared biological process in a microbial community? What is in it for the producer to share? Accident? Michi Taga 
ME: strains from outbreaks became virulent due to phage insertion of new genes. 
Not mutually exclusive. This argument before in bacillus competence
Besser: Every year 1 in 6 Americans (48M) get sick and 3000 die of foodborne illness. $77B/year cost. 
Why do cells give up corrinoids if expensive to make? How cells use them once they take it up different question Michi Taga 
ME: "mobilome" is made up of genomic pieces moved about by phage. 
Competence in v. cholerae induced by chitin.  DNA uptake. Why? Nutrition or diversity (or both) 
Besser: sushi "I'm pretty sure none of us wuld have been eating ground tuna from India had we known it was ground tuna from India"
Mark Eppinger of U Maryland Med- Using high throughput sequencing for SNP discovery in EC0157:H7 
Treponema primitia in termite gut Jared Leadbetter gave it to them. Michi Taga 
Besser: PulseNet has been able to detect multi-state outbreaks, may have been missed pre-1996 
Brian hammer from Georgia tech hgt in vibrio on chitin surfaces
  after this food borne illness session I will be passing on the open M & Ms for a while. Thanks for offering though!
It's quieting down at   and I are lonely in booth 1306
ME: looking at SNPs, building trees with the variations, mapping phenotypes on top. 
Besser: Of 10 largest outbreaks, 7 probably wouldn't have been detected w/o current surveillance techniques 
Toby Kiers: note that hoarding phosphate as host-inaccessible polyphosphate drives up its value and gives adv. vs. other fungi
How do they share the corrinoids? Wait, why do they want to? Are they stealing it? Cooperation without benefit unlikely Michi Taga
Hungry? Pickup some M&M's on booth 936 and learn about MacVector 12.6 too. 
 Wachsmuth says she has high hopes for USDA Modernization Act. I hope she is right. It is important legislation.
Mark Eppinger RT : ME: looking at genome plasticity in 228 strains! Many are from human outbreaks. 
  frm Korea: Lactobacillus GG probiotic alleviates IBS symps, lowers putrefactive bugs in poop. But worrisome inc in inflammn
Diff. microbes prod. diff. corinoids diff structure, diff quant, tons in termite gut Michi Taga 
Toby Kiers: Plants obviously can "offer" fixed carbon. associated fungi take 2 diff strategies. Freer sharing and nutrient hoarding
ME: looking at genome plasticity in 228 strains! Many are from human outbreaks. 
Cool! Inoculate germ-free mice with human gut microbiota to create exp system. 
Really sorry to have missed phage history session; appreciated the tweets by  
Toby Kiers; Power Struggles in the rhizosphere. root fungi and host plants enact strategies that prevent "enslavement" of either.
Corrinoid exchange as a model for moleuclar interactions in microbial communities some use those produced by others B12 Michi Taga 
Mark Eppinger, Univ of Maryland School of Medicine, E. coli O157:H7 
 it is amazing to me that sprouts keep popping up as the culprit in food outbreaks. Obviously we have work to do here. I'm off them!
Wachsmuth: German risk assessment: no good way to eliminate any bacterial pathogen from seeds to sprouts. 
Human virome dominated by bacteriophages; strain stability and high prophage suggests temperate lifestyle in gut 
Poster is done, that was fun. Now on to what species pan genomes
Really enjoying meeting everyone this week! Exhibit hall closes in a half hour. See you tomorrow! 
Wachsmuth: Foodborne outbreaks 2010-12, sprouts major culprit. "We haven't learned." 
What is the benefit of hoarding? Fungus stores P and then gets more C from the plants Toby Kiers 
Reduce run-times w higher G-forces w lightweight carbon fiber centrifuge rotors  booth 1015
  if you are knitting during a session I will pay you for the sweater. I am freezing!
Could we breed symbionts that give more more P for C and help feed the world? Toby Kiers 
 with all the new egg regulations out in place in 2009 how can we have had an outbreak infecting 1900 in 2010? Enforcement maybe?
If you knit while you are "listening" to a talk, the sweater will be too small, and you'll hear nothing. 
Alejandro Reyes - A Gnotobiotic Mouse Model for Characterizing Phage Bacterial Host Dynamics in the Human Gut 
 flying in! "chemicals from agricultural waste" leading so far...Disagree? Then vote!  ^kl
Mutualisms generally have one partner in control, but not always, can have economic markets also. Toby Kiers 
This talk just shows what you can do with a great system, careful ability to track resources, and theory and empirical. Toby Kiers
 OMG lady across from me is sleeping so hard she is about to fall out of her chair!
Strain variations in S. epi: phage and drug resistance (mec), biofilm, and transporters 
What happens to the P? Because it was stored in an inaccessible form, did not get more C therefore. Toby Kiers 
Both the cooperative and less-cooperative species made decisions on cooperation, responding to C from plant. Toby Kiers 
Was great meeting ": Delegates at ASM stopping me to pass on thanks for the superb SGM meeting,Dublin "
Wachsmuth: Japan initiated a project to preserve samples of all foods served in schools for 2 weeks in case of outbreak investig
Cool thing about this system of C trading for P is that the different species are differently cooperative Toby Kiers 
Infer phage dynamics from metagenome data 
Hmmm, if we can't photograph, and we can't record, why is it ok to tweet? I know Toby is fine wi/ it, but how about ASM? Toby Kiers
Clustering algorithm allows for binning with highly similar sequences. Identify strain variation, core genome and variants 
Delegates at ASM stopping me to pass on thanks for the superb SGM meeting, Dublin this year. Well done SGM! 
Effective enforcement depends upon the scale of interaction - could they discriminate neighboring rootlets? Toby Kiers 
RT : Infant gut shifts w nutrition and intervention
Kiers et al Science 2011 shows story on plant ability to allocate more C to the cooperative strain, Glomus intraradices Toby Kiers
Strain level determination based on abundance patterns. This seems computationally challenging 
 Toby Kiers Use stable isotope probing, see cooperative, less coop, least coop. Can track realtime C alloc of 3 dif. species.
 lesson - wash out the truck between loads! I remember that one... 
 Toby Kiers Where does the carbon go? Who does the plant give it to? Can the plant decide? Use 13Carbon dioxide.
Id citrobacter strains from metagenomic data  newer study more samples, more sequence 
 Toby Kiers Who is in control if a plant is hooked to several mycorrhizae and vice versa? Look really carefully at nutrient flow.
 innate immunity and pathogens. Listeria on stage
Wachsmuth: reminder, worldwide 1.8M children /year die of diarrheal disease, many of them food/water borne 
 Toby Kiers How do hosts enforce the cooperation of the partner, host is plant and other is fungus. Did careful exp. on nodules.
Wachsmuth: 1994 ice cream outbreak. Salmonella enteriditis, 224K cases, tankers contaminated with eggs -> ice cream -> people yum
So many topics and interesting posts from  unfortunately work calls me home
Infant gut shifts w nutrition and intervention 
 Toby Kiers Fair share hard when different things are given, carbon from plants, P, N, water, protection from the fungi. Confusing!
 Toby Kiers on cooperation and conflict in arbuscular mycorrhizae. The rhyzosphere is a competitive place. What is a fair share?
Yesterday afternoon we were put off pets and travel.Today it's ice cream... 
Come visit us at  booth 1306 and you might win a prize plus you'll get to meet the people behind this feed!
RT : Summers: electron microscope really cemented idea of phage as virus instead of enzyme 
  UNC Chapel Hill study: Livestock industry workers+families carry more MRSA nasally than do antibiotic-free farmers+families
Strain-level variation may enable genomic flexibility and adaptation in the face of selection pressures 
Variation at strain level allows pop to respond to selection. Culture, 16s, trad metagenomics won't cut it 
Sharon notes that strain level variation matters as it can result in substantial phenotypic differences 
2nd ex s. epi makes protease inhibits s aureus biofilm strain variation in ESP protease 
Prochlorococcus "the most abundant organism on Earth"
Itai Sharon talking about infant gut microbiome. Finding strain variation in metagenome finds islands ex. Prochlorococcus
Oh heyyyyyyy just won a Starbucks gift card at  from Thank you!!
Microbes are social creatures, I say hello to mine every morning when I get into the lab… sometimes they even say hello back ;)
There's still time  today! Stop by booth 1625, learn about  tool & get a chance to win an iPad. See U ;)
Itai Sharon - Microbial species and strain variations during infant gut colonization  
I'll cover the session titled "Evolution and Development of the Microbiome". Most notes will be at FF: 
Back for the afternoon session after some lively discussions over posters at 
Holding a 454 chip. Interesting to see where our sequence comes from.  
True RT  Many posters on culture independent MALDI–TOF MS microbe identification. Cheaper & faster than sequencing?
Next up at : Kaye Wachsmuth talking about foodborne illness & microbial risk
Last session of the day: microbial powers to tap Earths energy.. Battery dying so no live tweeting - will post some thoughts later
Come hear award winner Kaye Wachsmuth’s lecture “Foodborne Illness and Microbial Risk” in the Esplanade Ballroom 304 at 3 pm PT  ^lg
 has you sold on NGS. Find out how easy Nextera smpl prep is for sm genome DNA, PCR amplicons & plasmids
Been a busy day here at . Lots of follow-up to come for our sales team!! 
  I may construct a blanket from all the bags the vendors are giving away. 
 I must have missed you! I'm at my poster now 1792 and will be around until Wednesday 
 not to complain but if room 304 gets any colder I will begin hibernation.
Still more chances to win a kindle at  - pick up a pin at the Cell Press booth (1211)
Award winner, E. Peter Greenberg, is giving a lecture at 3 pm PT in the Esplanade Ballroom 305/307  ^lg
Just gave away a kindle as part of the Cell Press (booth 1211) pin to win contest! So exciting!  
": All at  pls check out new microbiol twitter jrnal club   Next session tom 12pm SF time"
  copper in tissues (& drugs which boost Cu) kill TB bug!   Novel approach in anti-TB chemotherapy
  Shit! No, literally, human fecal extract reduces sensitivity of gut bugs Salmonella Listeria to fluoroquinolone antibiotics
Find out how  can enable small genome rsch with the fastest & easiest workflow. Booth 310 at  or 
..always someone who spots that ;-) RT : The blue banner in this booth also has a left-handed DNA. 
All attending  pls check out new microbiol twitter journal club   Next session tomorrow 12pm SF time
Hope everyone's having a great time at ! Don't forget to stop by our booth #1131 for samples and giveaways!
New  Piko Real-Time PCR System has 5 channel detection for multiplexing & flexibility  booth 1015
Bacterial cells "duel" with each other with T6SS. Awesome!
Have to say this again...Mekalanos's T6ss real time videos are incredible!!! 
First trolley ride in San Francisco was definitely an experience 
Mekalanos: real time videos of Type 6 SS action. Quite awesome!
Superbug testing: way forward? MT : RT  Real-time gene sequencing used to fight 
I want one! RT  At  Illumina announced new mini MiSeq sequencing system - going to change world
RT : This is just, ew. MT : Ross says bottled water she surveyed has 100x as many microbes as tap water
 Yep! If by water cooler you mean dark room of seated people looking at Scott :-) Thanks for the link - that's great.
John Mekalanos: RNA-seq-based study of V. cholerae in vivo gene expression in mouse, rabbit models 
Busy day at  I'm sad I have to leave San Francisco so soon! But at least i see  tonight for our
  Many thanks! Your notes are awesome and greatly appreciated ^_^ 
RT : Disappointed to see this big turning metal DNA model is left handed.  Do microbiologists care?
ES: knocking out different methyltransferases in outbreak strains to assess effects 
 nice to meet you . Congratulations to you and your colleagues for providing an excellent meeting. 
Ahh - the new Illumina mini MiSeq is so easy to use even children can sequence microbes with it 
 2.0 is here! The next generation of handheld, automated counting   Booth 1523
Schadt: found methylase that targets every A in E.coli genome. 8 other new methylases 
Have attached mini Illumina MiSeq I got at  to laptop but does not seem to be downloading any sequences
 Uh oh. Is this now water cooler talk around ASM? :) 40% Wolbachia frequency was just published. 
Representin at  booth. Visit me-row 1600. See my giant  pic-Brad Goodner w his poster! 
ES: re-analyzed German E.coli outbreak strain to ID variation in kinetic signatures 
The Illumina mini MiSeq I got at  has a thumb drive port but I can't figure out how to get DNA into it
Cute name, seriously hard-working anaerobic incubated workstation. See the Bugbox at booth 1534. 
 Think I saw you yesterday but got distracted. You probably won't recognize me (long hair, glasses). Hope to see you again 
Wow RT : At  Illumina has announced a new mini MiSeq sequencing system -going to change the world
At  Illumina has announced a new mini MiSeq sequencing system - going to change the world 
 Surely Scott appreciates the generosity but do set the man straight. Soon. He's telling, well, everybody :-) 
Eric Schadt discussing application of PacBio SMRT in real-time pathogen surveillance 
Poster time! Genome evolution in SAR11- 2092. Come say hi.
The  Smart-Vue wireless monitor has capabilities across temp controlled  equip  booth 1015
Disappointed to see this big turning metal DNA model is left handed. Do microbiologists care?
Weiser: Host: antibodies (IgA1) clump bact. cells to target. Bact: protease targeting Ab hinge. Host: make Ab's against protease!
Re  session on microbes in the built environment more info on this topic available at  and
At ? Visit the NIAID booth to get information about the Common Fund high hist high reward programs! Booth 715
Weiser: However, in nasal passages, large size of S. pneumoniae selected for because promotes adhesion to epithelial cells
Weiser: small bacterial size selected by complement/neutrophil immune surveillance. Bacteria marking/killing prop'l to surface area
Jeffrey Weiser: S. pneumoniae colonizes 50-100% infants, 10-30% adults. Commensal/pathogen. How does interact with immune system? 
Come by the  CM Lounge (rm 122) at 1pm to meet Dr. Cornish and Susan Munro and discuss Clin Micro careers! ^tg
Bevins: no fungal/net follow up? Cool: blinds analysis to source of SEM images, asks to assign to source strain, measures accuracy.
Visit booth 1126 to learn how Life Technologies is advancing microbiology research 
In case you are wondering, Eric Schadt's talk is 1PM in room 103. followed by Mekalanos at 1:30 
Anyone else around? Meet here at 1! RT  middle entrance at 1, for hall B. 
Bevins: SEM revealed "netted" fungi. Ssowed DH6 self-assembles into nets in presence of bact. surface proteins. What about fungi?
Bevins: HD6, though, took many grad student tears to figure out. Clue: cloudy white supt in fungi exposed to purified protein
Amazing talks so far at ! I'll be at the Cell Press booth (1211) from 1 to 2. Come say hi & pick up some freebies
Bevins: HD5 forms dimer with alternate exposed hydrophobic and charged sides. Embeds in microbial cell membranes (like porins?)
Charles Bevins (UCD): human defensins (HD) 5 and 6 for "harpoon" and "net" when host detects microbes in Paneth cells (crypts).
Using Illumina NGS to track MRSA in real-time: study from Sanger, U Cambridge & Illumina just in time for  
Total attendance at : 9,082 (highest since 2008); scientific attendance: 6,590
 Many posters up today about use of culture independent MALDI–TOF MS for microbe identification. Cheaper and faster than sequencing?
Have questions about next-gen sequence analysis? Katie and Stephanie will be happy to answer them in booth 1518 at .
Great to finally meet you! RT“: What coincidence, sitting beside  during Summers talk on phage history
Enjoying  microbiome tweets from home but disappointed in lack of plant microbiomes
  C neoformans, clever bug, subverts mny host physiological components- brain inositol, Scavenger Receptor A -2 its advantage
Bosch: like Karen Guillemin in zebrafish, sees reproducible migration of hydra microbiome composition vs embryo age across PCA plot
What coincidence, accidentally sitting beside & meeting during Summers talk on phage history 
RT : Loss of wetlands can mean higher levels of parasites moving from land to sea; less filtering 
Learn something new everyday. Great live talks . Hope to catch tomorrows ones as well. :)
Thomas Bosch: massive die-off from fungal contamination - Fusarium. Hydra microbiome=potent source of antifungal small molecules. 
Thomas Bosch: develop gnotobiotic hydra system to test host-microbe interactions. Surprise: antibiotics => massive host death!
Maloy: Tracy McNamara played fundamental role in West Nile virus, have video interview from last year 
Free Laptop covers celebrating journal Pathogens and Disease, come and get yours now! Stand 724 
Gurfield: Last year, talked about microbes influencing formation of hail & rain, One Health hasn't begun to address that yet 
Dr. Robin Patel presents "Microbiology Literature Review 2012" today at 3pm. 
Harrowing truth regarding small villages in the developing world
Atlas: Heard that we should vaccinate animals first and children later, as the animals would feed the entire community 
Nicole King: do modern microbe-host interactions resemble ancient ones driving evol'n? Setting up experimental system to test
RT  Get highlights from  day 1 and up to the minute news from Twitter with our Live at ASM coverage:
envious of everyone attending  in san fran. many great talks! one day we will meet... one day...
Atlas: Schwabe at UC Davis has tried to unite animal and human epidemiology 
 poster 1921 Phylogenetic Analysis of Kenaf Fiber Microbial Retting by Semiconductor Sequencing of 16S rDNA Amplicons 18 June 1:00PM
Atlas: Pasteur & Koch were looking at animal & human diseases, rabies, Koch kept animals next to his practice. Siloed after
Question for the panel of ASMlive: where wd u spend money to improve  research? 
Gurfield: Rudolf Virchow at the end of the 1800's was proposing looking at One Health. Now int'l travel & commerce requires this
  transcriptional diff b/w C neoformans isolated from male & female HIV infected ppl. Crypto meningitis risk higher in males
Just saw Hazel Barton. Cave micrologist-adventurer! 
Nicole King: reviews choanoflagellate rosette formation stim'n by bacterial sulfonated polyamine finding (tweeted yesterday).
Gurfield: Veterinarians are more used to crossing sectors than other fields, due to having to manage health of herd or flock 
RT : Disease-causing agents on land can affect health of ocean inhabitants 
RT : Oocysts from cat feces likely source of T. gondii for otters 
Nicole King: spectcular talk suggesting that microbes drove evolution of multicellular life. 
RT : Otters die from not only T. gondii encephalitis, but those infected also 3.7x more likely to be chomped by sharks
Get highlights from  day 1 and up to the minute news from Twitter with our Live at ASM coverage: 
Gurfield: One Border One Health was started in San Diego a year ago, per border btw San Diego and México emerging diseases
RT : Sea otters endangered by toxoplasma gondii encephalitis, federally threatened species 
Hadfield: settled colonies => "hull fouling." US Navy estimates this costs $0.5 billion/yr. and their fleet is 0.5% of world fleet! 
RT : First up: Patricia Conrad, Division Z lecturer on Otters, Oocysts and Oceans 
Atlas: Much better communication w/in public health. However, big division btw medical field and public health. 
Q: Have officials, vets and public health officials, gotten better at talking to each other after West Nile? 
Michael Hadfield: bact. colony attractor can be from single species;others do have activity. Are larvae hijacking biofilm glue?
 poster 2101-ID of Gene Repertoires Responsible for Persistence of Global Pathogen Across Niche Dimensions- 18 June 1:00PM
Atlas: Today, the environment is the least understood part of the OneHealth equation. 
Atlas: WWI microbes were used against donkeys and horses, the cavalry of the time. Only later used against humans 
Sars and wealth, I'd never even thought about it. Learn something new everyday 
Lipkin: SARS spread most easily in the French hospital that had the money for nebulizers and so forth 
Michael Hadfield: bacterial biofilms induce settlement of very diverse set of marine larvae: sponge, coral, mussels. And bryophytes
Lipkin: Diseases of wealth: see organ transplantation, treat cancer, more immunosuppression: context of more virulence 
Gurfield: B/c we're in a wealthy society, we care for exotic animals in backyards, and thus gave an elephant MRSA, who gave it back
Lipkin: Will need to sample at ports of call. Gets to thorny issues of human subjects' freedom 
Lipkin - diseases don't just emerge from jungles - think daycares and animal farms too 
Lipkin: Need to sample day-care centers. Have good models of emerging infectious diseases: population density, access to bushmeat 
Remarkable (and at times quite speculative) session on how microbes might have shaped evolution of multicellular life. 
I am loving the contagion refs. Go Prof. Lipkin. 
Oops, previous tweets on big data were Lipkin, not Gurfield
Lipkin: Filmmakers were committed to making Contagion educational about One Health 
RT : Summers: electron microscope really cemented idea of phage as virus instead of enzyme 
Maloy: Lipkin was scientific advisor to movie Contagion, which educated about One Health 
Gurfield: Will need whole new population of people who understand biostatistics, data management, etc. Need people who can use info
  Meth alters blood brain barrier integrity to facilitate brain infection by bugs including C neoformans
Gurfield: Have not sufficiently invested in computational power and people to analyze big data. Tiny percent of health budget 
Tree oil may combat obesity, diabetes (cool research by S&T team led by env engineer  
Gurfield: Revolution due to culture-independent techniques, sequencing costs dropping well beyond Moore's law. Challenge of data 
Lipkin: MRSA moved from human to baby elephant, was amplified, then infected humans taking care of the elephant 
Likes the acknowledgement that its an exchange that flows from human to animal and animal to human 
Totally agree with panel that we shd consider zoonoses as 2 ways and not just animals to humans 
Atlas - zoonoses 2 way street RT : ASM Live – One Health: Humans, Animals and the Environment 
Lipkin: Eco-tourism causes outbreaks of menopneumovirus (sp?), dangerous to neonatal apes. 
Atlas: Formal definition of zoonotic is unidirectional, but it's an interactive exchange. Reverse issues? 
S&Tresearchers report 'Almond Tree Oil May Combat Obesity, Diabetes':  
Lipkin: West Nile virus came to US shores 1999. HIV, influenza viruses that strike yearly, encephalitis, hantaviruses from animal
Lipkin: 70% of infectious diseases rooted from year to year in animals. Agents may also move from human to animals & back
Watch ASM Live Now: One Health: Humans, Animals and the Environment   ^js
Maloy introduces Ian Lipkin, Ronald Atlas, Nikos Gurfield on One Health: human, animal and environmental 
Watching now! RT : Watch Now! ASM Live – One Health: Humans, Animals and the Environment 
Watch ASM Live Now: One Health: Humans, Animals and the Environment   ^js
Stanley Maloy, chair of ASM communications committee, starting next session of ASM Live 
": Watch Now! ASM Live – One Health: Humans, Animals and the Environment "
  avirulent giant "Titan" cells formed by C neoformans; act as big brothers 2confer protection from host immunity 2smaller CN
Watch Now! ASM Live – One Health: Humans, Animals and the Environment  
-we enjoy it a lot! Come meet us at booth 1625. We'll explain all about   tool & give U the coolest gifts
Your cold cuts are treated with anti listeria phage, phage history lecture 
Still time to join us in the audience for ASMlive 
 Ah thats a much better viewpoint. ~stops glaring at chinchillas~. Immune advantage through exposure 
A century of bacteriophages... not just campfire stories... @
Summers: "The world's a phage" Incredible abundance, diversity
 I take the other route which is to assume that my vet practice time means I am infected with everything already!
Its sad how happy live coverage of  is making me. Stress levels dropping nicely. (I am such a terrible nerd)
Go and see  talk about how Horizontal Gene Transfer has Shaped Public Goods Games in Osmotrophic Eukaryotes at 5 
Go and see  talk about how Horizontal Gene Transfer has Shaped Public Goods Games in Osmotrophic Eukaryotes at 5 
 Very true ~looks at pets with suspicion~. Resisting the urge to set up screening procdures for the small zoo I live in
New  Barnstead GenPure xCAD extended Control & Dispenser unit, increases room on  bench 
Summers: "gadgets" used to study mutations (eg Luria-Delbruck experiments), protein and nucleic acid synthesis 
Summers talks about Sinclair Lewis' Arrowsmith. Early example of influenced by discussions with Paul de Kruif.
Summers: development of EM by Siemens in the 30s finally settled the microbe vs enzyme debate 
Summers: electron microscope really cemented idea of phage as virus instead of enzyme 
Eisen Sightings! Times three actually. Nice to see you and your F1s, Jon.  
Summers: Phage mid-20th C, used as "gadget" of molec bio
RT : Summers: Phage therapy seen as panacea early on, even inspired a novel 'arrowsmith' by Sinclair Lewis won a ...
'Arrowsmith' movie shows early example of being scooped. On Netflix apparently! 
Side note, need to watch "Arrowsmith" movie for all the science-y badness. Hype, media, bad acting, looks awesome & on Netflix!
 looking for a video of IAN LIPKIN: One Health: Humans, Animals and the Environment
Summers: book made into a movie, was  nominated - great clip   looks like accurate biopic of lab life 2 me!
Summers: moved to pop culture. By 1925 phage in "Arrowsmith" by Sinclair Lewis, made into movie 
Summers: Phage therapy seen as panacea early on, even inspired a novel 'arrowsmith' by Sinclair Lewis  won a 
Summers: By late 20s, looking at phage as treatment for disease. Why trials? No antibiotics, nothing else worked, desperate
Summers: ah, drama. Scientists fought amongst themselves for credit of phage discovery, set back research 10 yrs, microbe v enzyme 
Used both culture-dependent and culture-independent methods, checked resistance of isolates phenotypically and looked for genes
It is official, house flies are gross and harbingers of disease
Study showed humans swallow 3 spiders per night during sleep (????!!!!) 
A: Haven't looked at food chain, don't know about spiders, but do know about human food chain, do get antibiotic resistance
Q: Transfer of antibiotic resistance through food chain, e.g., spiders eat flies? 
Next to trash can in residential area, lots of houseflies. Fly may land on your skin, you may touch your face... 
Another reminder that pet ownership comes with risks at ASMlive
Summers: Plaque assays are a remarkably stable technology, unchanged since 1922! 
A study has shown transfer from housefly to cattle of significant # of bacteria in single landing 
Tree oil may combat obesity, diabetes, Missouri S&T research suggests presented at  
Nice to see so many people who appear happy to see me at the poster session. 
Summers: d'Herelle 1922 experiments using plaques--same technology as today "hasn't advanced since 1922" 
Summers: d'Herelle, 1917--conceived of phage as a virus of bacteria rather than enzyme 
Maloy quotes Julian Davies, "The world is bathed in antibiotics"
Have tried to compare with more pristine places, e.g., Yellowstone National Park, though not as wild as coral reef 
Summers: d'Herelle was a deserter who may not have finished school. Set up lab in own basement 
Focus on commensal bacteria as their chance of transferring to pathogens by horizontal gene transfer is greater 
Lactic acid bacteria and enterobacteria can be opportunistic pathogens, e.g., human upper urinary tract infections 
A: Have identified resistant carriers by genes, find they are the same ones as found in human host commensal bacteria 
House flies can transfer antbiotic resistant bugs to uncovered food
Summers: Twort, early phage researcher (by accident)--found "transmissible lysis" thought to be due to enzymes 
Q: Have seen transmission? A: In housefly study, have seen transmission of pathogenic bacteria from fly to ready-to-eat food
Lots of great tweeting from  -- keep up the good work!
60 billion pounds of animal waste are produced in the US annually. They become source of resistant bacteria. Flies breed there
Listening to William Summers talking about "A Century of Bacteriophages". I love the  history lectures!
Flies are an important dissemination route of resistant sources from farms and hospitals to the human community 
Summers: Early phage researchers struggled for funding/keeping a job for more that a few years. 
They looked for erythromycin and tetracycline resistance - used in agriculture. Also looked at cephalosporin resistance 
Found coding genes for antibiotic resistance from houseflies from all areas. 
 Thank you for linking the live  coverage, really enjoying it. :)
 Free coffee and cookies to celebrate journal Pathogens and Disease. Come to Stand 724 now and talk with Chief Editor Patrik Bavoil
Houseflies in both farms and residential areas carry antibiotic resistant commensal bacteria; more prevalent closer to farms
Non-food animals: pets, zoo animals, and houseflies, provide reservoir of antibiotic resistance even w/o being treated 
Antibiotic resistant bacteria can be carried by houseflies 
80% of antibiotic use in the US is in food animals, then humans in hospitals, then humans in community 
 attendees: Interested in publishing in an journal? Come meet IAI production editor Diane Smith, booth1308 til noon.
Ab resistant organisms in environment are kind of like ecotourists.
Visit Lathrop Engineering's booth #523 at the  to ask questions & learn about our newest development solutions and capabilities
Summers: Size of audience suggests history alive and well at. More importantly, suggests that phage are coming back in a big way!
Antibiotic resistance in non-food animals being covered at ASMlive now 
 hitting the product show to get my schwag. Hope for more Starbucks gift cards!
ASM Live from . Role of non-food animals spreading antibiotic resistance.  Watch it now ^js
ASM Live from . Role of non-food animals spreading antibiotic resistance.  Watch it now ^js
Stop by  booth 1015 to check out Spectra MRSA, the source for  testing: 
Watch Now! ASM Live – The Role of Non-Food Animals in the Spread of Antibiotic Resistance at 
If u planned to buy a copy of "Microbes and Evolution" @ u must shop fast! We only have 10 copies left at the ASM Press bookstore!
Anyone looking for good coverage of  should check out, shes doing a fantastic job.
Amazing battles described in  session on "Microbes trigger and shape immunity."  Fun taking notes w 
New symposium: a century of phages by William Summers, then off to posters 
I'm pretty sure that  is one of the only places in the world where everyone washes their hands before leaving bathroom
Now for a change of pace with a talk on a century of bacteriophages by Bill Summers 
It's another productive and exciting day in San Francisco! Ask Betty or Barry for a demo of the anaerobic Bugbox at booth 1534.
 exhibits are open! Come to 310 to find out how easy it is to get started with NGS. Fill out survey & have some  on us.
A great set of talks so far. Now to ASMlive for antibiotic resistance
Clin Micro Mentoring Session @ 11 am! Stop by  CM Lounge (rm 112) to meet Dr. Viscount and Brent Barrett!
Patrice Couralin: correlation between plasmid copy # and vancomycin resistance. Fortunately resistance is costly; slower growth 
Melissa Karau talks Treatment of Methicillin-Resistant Staphylococcus Aureus Experimental Osteomyelitis at 1pm.
VA: diatoms hang out where nitrate meets iron, specialized regions on both sides of the Pacific. 
Starting second day at . Stop by booth 1617 for special discounts on microbial genomics products
 in addition to booth 1623, if you catch me elsewhere, I can demo most of the PortEco/EcoliWiki phenotype tools on my iPad.
 sounds like "niche-uh". N only gets consumed when iron gets brought in. 
According to Jeff Weiser, size matters. Smaller pathogens are harder for the immune system to clear 
VA: diatoms use iron for their nitrate assimilation pathways.
MT : Charlotte Sage is at  through tomorrow. Need to contact her? Follow us and go here: 
Charlotte Sage, a member of , is at  through tomorrow. Need to contact her? Follow us and go here:
Background reading on PG synthesis part of Gross talk, from Vollmer, Gross herself and their colleagues 
VA: diatoms can grab the abundant nitrate because they turn on the genes, other organisms don't. 
VA: 3817 genes differentially expressed, match predicted taxa for iron fertilization: diatoms 
VA puts up a plot from Marchetti PNAS 2012 with hundreds of points where EACH ONE is a pie chart! 
 Angela Douglas: metabolic pathways are shared between symbionts. There you go, KEGG...
Jeff Weiser talks about how Streptococcus mutants are subject to "Fantastic Voyage" syndrome, resulting in immune clearance.
VA: mimicked iron fertilization in seawater, filtered for metatranscriptomics, examined microscopically. 
Gross: few KOs with many phenotypes (envelope, transcription, translation). Many more with multiple phenotypes already annotated
VA: ferritin allows iron storage in a small group of diatoms making them dominant. 
VA: dominant bloomer is pseudo-niezche (sp?), make a unique protein called ferritin 
Looking forward to a century of bacteriophage plenary at shortly. Fascinating little things.
The microbiota is not always beneficial to the host 
  Thanks to y'all for liveblogging  So near and yet so far!
 come by booth 1623 if you want a demo of how to search/browse the high-throughput phenotyping data Carol is talking about.
VA: iron is what limits phytoplankton in the northeast Pacific Ocean.
 Sorvall MTX Benchtop Micro-Ultracentrifuge can deliver 1,048,000 x g. Chk it out booth 1015 
Gross: phenotypes for half of E.coli genome in at least one environment 
 what are the scenario in Asia? especially in South Asia?
Levine  systemic Salmonella strains increased pseudo genes - loks like captured in evolution to host specificity
Carol Gross' group screened 4k E.coli mutants under 120 unique stresses using comprehensive interaction profiling approach
Gross: finely arrayed E.coli deletion library (1536 KOs per plate). Screen for growth under 300 conditions 
Levine: animal reservoirs also not found for NTS in Africa, appear to be H2H transmission instead of zoonotic like in US 
Levine: Most African strains are typhimurium & enteritidis, causes of serious invasive disease, genomics look like typhi 
VA: diatom genomes range from 26-219Mb. The big one is highly repetitive, lots of transposons, like maize 
Talk by Jeff Weiser (U Penn): ... and Immunity shapes microbes 
Levine  incidence of systemic disease by nontyphoidal Salmonella increasing, serious problem inAfrica
VA: diatoms with a urea cycle- what is that for? Still unclear- why would there be a N waste product in a photosynthetic organism?
 Carol Gross. Phenotypic signatures more powerful than single phenotypes. This data is browsable via PortEco
Levine: in Africa, high case fatality with non-typhoid salmonella, don't present w/gastroenteritis, weird epi comp. to US 
mT  tribute to Maurice Hilleman before introducing Mike Levine as winner of the 2012 Hilleman/Merck Award. 
I can't decide if  is making me feel better or worse about missing the meeting. Keep 'em coming!
RT  Rapid whole genome seq for neonatal MRSA investigation using . New article in  today: 
Levine: non-typhoidal salmonella--zoonotic, transmission by food products. Occasionally invasive. 
VA: diatoms have primary and secondary endosymbiosis, along with bacterial genes. Lots of contributors to their genomes 
  as much as single dose recombinant oral vaccines are welcome I wonder about issues around live virus field storage logistic
see also  RT : Abt 50% correct GO annot, more nearly right. Not bad. Compares well w machine annot"
VA:  Marine Microbial Transcriptome Project will help a lot- in progress 
Feedback issues with the audio in this session, sounds like the speaker system is about to blow!  ahh, fixed now...
Loving tweets from . Hope the  crowd incl. are having fun & recruited lots of new members!
 More sound problems during Carol's talk. wtf?
VA: very few diatom genomes, existing ones are highly divergent.
 attenuated recombinant live vaccines for paratyphoid that are single dose oral administration would be great N travel medicine
Levine  new Typhoidvaccines promising initial studies on effectiveness and tolerance
Angela Douglas on health consequences of resident microbes now
 Carol Gross on bootstrapping our knowledge of bacterial models
 I missed seeing your poster yesterday at ! The evening "poster session" was a complete ghost town, w/ very few posters :(
VA: diatoms are no more closely related to plants than you or I
VA: showing crazy starburst tree that is Eukaryotic phylogeny
So funny to read my twitter feed and realize  is at the same session as me 
Levine: 2 vaccines but not great, infants can't take & many doses required 
 Christopher Lee on phenotype sequencing - like GWAS for bacteria. Id. mutations assoc w phenotype for $100-300 by pooling strains
 questions for Chris Lee reminds me of classic Jeff Miller papers on saturation mutagenesis of lacI
Last but not least, Carol Gross talks on systems approaches to dissect bacterial cellular processes 
Levine: chlorinated water & filtration in early 1900s = huge drop in typhoid transmission 
Had never occured to me transcription would continue post death.cert never thought some genes wd be upreg! 
Levine on Salmonella  ... Yes, pseudogenes and host specificity prediction from genomics BUT proof required genetic tests!
Today at the  Clin Micro Lounge (rm 112) -- learn more about career opportunities for CMs!  ^tg
At 1 PM today  - Eric Schadt - Whole-genome Characterization of Methyladenine Residues in Pathogenic Strains
Levine: Many places to intervene--water, carriers, etc. Vaccine to help eliminate carriers. In past, rivers source of outbreaks 
 Domazet-Loso: dead = extended life cycle. Zebrafish is dead, cells not directly. > 100 genes are upregulated - 48 hrs. Why?
MAM: turns out the important Roseobacter species off CA is one of Steve Giovannoni's: HTCC2255 
The postmortem microbiome. you get eaten by microbes 48 hrs after death. 
Immune response, inflammatory upreg postmortem. Also look at microbiome. Again is this deep or obvious? 
MT : Domazet-Loso:zebrafish look dead, but how do you know theyre really dead?more than 100 genes upregulated after death 
Lee: NGS reliably and cheaply detects top genes that contribute to mutant phenotype. Pooling is a win/win for cost & thruput 
MAM: monitoring dmdA genes with automated Environmental Sample Processor from MBARI. 
Christopher Lee calls for collaborators to try out their phenotype sequencing approach with 
Most of the genes just go away, about 100 go up 
Levine: Salmonella as emerging pathogens. Typhoid--emerged in 1800s, restricted to humans 
Domazet-Loso: the zebrafish look dead, but how do you know they're really dead? more than 100 genes upregulated after death
CL: approach could expand the scope of mutants one might analyze
Postmortem microarray. Put zebrafish in cold water to kill, then back in warm. 
Correction, bacterial switch is between cleavage of DMSP to DMS vs demethylation to MMPA and finally CO2/acetaldehyde 
Domazet-Loso: how do you kill zebrafish without affecting their gene expression pattern? 
Be sure to visit the ASM Press Bookstore at . Members receive up to 30% on most books. ^csb
New counter theory to endosymbiosis after a few drinks at last night. Exogeneis: Expel all the organelles!
Hilleman Award  - "Vaccines he developed saved more lives than any other scientific discovery in the century"
Relate origin of oxphos with rise in O2 change in fossils that is cool 
MAM: 75% of DMSP is demethylated and consumed 25% to DMS. Some goes to atmosphere, some consumed. 
  You've been quoted in my  story: "CACAO at  see us at booth 1623" 
 Oxoid & Remel susceptibility testing products for easy interpretation & consistent results 
Evolution of endosymbiont oxidative phosphorylation see peaks in phylostratigraphy at origin of animals, animals and fungi 
Chris Lee: Isobutanol tolerant mutant strains 90 million single reads on GAIIx, fast & inexpensive way to det microb phenotype 
I'll be presenting poster (Div R 1546) on using electrophoresis of virus particles to examine sex in wild viruses. 
 very nice tribute to Maurice Hilleman before introducing Mike Levine as winner of the 2012 Hilleman/Merck Award.
MAM: metatranscriptomics showed carbon processing from DMSP was highly important at BATS 
  web app received top rating in GEN's Best of Web column! See you at  booth 1625.
MAM puts up a picture of work on the R/V Atlantic Explorer - I love that ship! 
Links origin of cancer w origin of multicellularity. Is this deep or obvious? Not sure 
Domazet-Loso says parsimony analysis of "cancer genes" puts all at root of metazoa and all metazoa can get cancer 
MALDI-TOF in Microbiology Lab. Join UNC School of Medicine Speaker at bioMérieux InBooth Knowledge Forum at 11.50am booth1301 
  the problem even more worsened because of not having research and let 'unknown' disease subside by itself 
Congratulations to Susan Sharp PhD recipient of bioMérieux Sonnerwirth Award 2012 for Leadership in Clinical Microbiology during 
Relate txome in hydra stem cells to phylogeny. More potent stem cells express earlier genes 
Excellent point regarding  "The sequence is not the goal" Dr. Lee   
Listening to my old mentor Dr. Mary Ann Moran speak at . Feeling a bit nostalgic for environmental micro
Charles Bevins notes that your Paneth cells are awesome, but might play a role in the development of Crohn's disease 
To make phenotype sequencing economical, 2x pooling — library pooling + pooling multiple tagged libraries per sequencing lane (CL)
 pooling strategy allows you to generate less sequence, look for variable bases
MAM: looked for DMSP demethylation gene dmdA in metagenomic data. Found many different clades in GOS data. 
Used txomics to find evidence of phylogeny ontogeny relationship in zebrafish  nature paper 2010
 about 30 mutant genomes allows you to use stats, 5 is too small. A function of number of mutations. Chris Lee
Domazet-Loso: genome phylostratigraphy: a way to reconstruct ancestral gene content, identify deviations from a null model
Look at trait over evolutionary time, compare across phylogeny, look for correspondence to evolution divergence 
 want to find point mutants with interesting phenotypes? Sequence the bejesus out of them + stats kung-fu = job done. Chris Lee
MAM: DMSP demethylation winds up producing CO2 and acetaldehyde 
  so many of our shared disease trace origins back to our sharing quarters with fowl, swine & bovine. even flu!
Christopher Lee discussing 'phenotype sequencing' — microbial mutant sequencing studies 
Come hear Patrice Courvalin, an award winner, present his talk about S. aureus at 10:15 am PT in the Esplanade Ballroom 309 ^lg
 overflow room for phenotype sequencing talk is pretty full!
I also note Steve Giovannoni's lab has isolated many many Roseobacters 
Genomic phylostratigraphy. Yikes!  arrow of time in genomes. Paging 
Come meet your ASM Ambassador at the Ambassador's Forum (Convention Center; Room 114; 1:30pm-3:00pm)  ^tp
MAM: Rugeria pomeroyi DSS-3 is their model Roseobacter
 I never have liked that lucky bamboo plant anyway. It isn't even a bamboo. Just pour out that Aedes reservoir! 
MAM: large gene expansion in deep Roseobacter branches, possibly correlated with emergence of phytoplankton 
Animal and microbial genomes domazet-loso from Croatia
Thanks Maryn! RT  i sincerely hope disease geeks are following  today as she tweets cool microbey stuff from
Nice talk  bet-hedging and the search for the coin-tossing gene Dr. El-Samad 
El Samad: no single bet hedging genes, rather there are bet hedging programmes to provide stochastic diversification in fungi 
 Come and get your free coffee and cookie from 11 - 1 Wiley-Blackwell #724. Meet Patrik Bavoil editor of Pathogens and Disease
 thanks for passing it along. Can get it to open in this room. Will check it out later. 
Gurfield: 2011 reemergence of albopictus in same area."Whoever thought owning plants was risk factor 4 exotic bugs" 
 and most people in developing countries spend entire life with to cattle. One health for everyone .
MAM: focusing on Roseobacters, found near or on phytoplankton
MAM: DMSP degrades to DMS which influences cloud formation. DMSP is an osmolyte produced by phytoplankton. 
  as if cock-fighting or animal fighting of any sort wasn't creepy enough the microbial ramification is scary.
Gurfield, case #3 Aedes albopictus. Outbreak in LA county, abopictus eggs came in with "lucky bamboo" plants 
Next, Mary Ann Moran on sulfur cycling through DMSP 
Missing out on ? Check out the videos of yesterday's sessions and watch today's live! 
RT  the movie contagion would have been much less scary if they used the comic sans font
Metaorganism (or holobiont?) is unit of selection. Change microbiome to adapt 
Learned the difference between apoptosis and pyroptosis. 
i sincerely hope disease geeks are following  today as she tweets cool microbey stuff from 
": Gurfield:  transmission human to elephant, then back among humans "
Gurfield: origin due to fighting cocks from Mexico, underground ring in CA spread infection 
Complex community necessary, biozoenosis -cool word. Also holobiont and hologenome.  
  lots of folks in this room who sleep with four-legged cats and dogs are cringing. One Health indeed!
Gurfield: 3.9M birds destroyed, $160,000,000 to eradicate, $121,000,000 lost trade 
Getting ready for another exciting day at  ! Stop by booth 1616 today & enter to win Beats by Dr Dre headphones!
Model supports active host role in microbiome formation. Microbe effects host also  
New award proposal: conference name badge worn farthest from the convention center, resulting in massive coolness factor.
Is "exotic newcastle disease" in birds what we call simply "newcastle disease" in the UK? 
 Thomas Bosch: hydra's grown without bacteria show fungal growth because bacteria are needed to protect!
Gurfield: Case #2, exotic newcastle virus, foreign animal disease, rarely zoonotic (conjunctivitis)  Outbreak in CA 2002-3
 I was not expecting that one!  MRSA transmitted human to elephant calf to human.
 just wanted to know: how visitors are responding to green batteries? 
 Oooh, thanks for that, will keep an eye our for his stuff. Am following all the  tweets from Davis, so keep posting!
 Well  is still down at , so you should totally hit him up for lunch/dinner/drinks!
KC: looks like AOA fractionation explains N2O production in nature.
Pradipsinh Rathod says intergenic AT rich regions serve as break points to increase copy # as drug resistance strategy 
KC: 70% of N2O produced goes through a proposed intermediate: either NH2OH or HNO 
Gurfield:  transmission human to elephant, then back among humans 
 first time in an overflow room at ASM - odd to see slides, hear the speaker but have an empty stage.
Nikos Gurfield  preemie elephant calf in close contact with human caretakers transmission of MRSA from human to elephant
Gurfield:  cutured from human & elephant (USA300). Rest of elephants were negative. 
Just had read article about virus behind vanishing bees. ": Can a virus save the honeybees?  "
Nope! “: Summary. Bacteria, is there anything they can't do?  
Sad to be missing out on the rest of  -- Headed to on Thursday and inundated with other-conference prep!!
KC: isotopes to track N and O fractionation from ammonia + O2 to N2O 
Sterile hydra attacked by fungi. Bacteria in glycocalyx coating protect  new antimycotics?
 session is full of pictures of gorgeous animals! 
Gurfield: Case of African elephant calf, skin pustules in animal & caretakers (guess what's coming) 
In absence of gut microbiota in fish gut epi doesn't develop. Squid light organ develops in response to v. fisheri 
KC: N2O was produced in parallel with Nitrite 
Morning - day 2. Come visit us today-booth 1625. We'd love to meet & show U   tool. Waiting for U there.
El Samad: Cell to Cell variability may be a regulated phenotype e.g. Dig1 story in yeast mating 
Stop by  booth 1015 at  for a look at the NanoDrop Lite, delivers accurate measurement 
 H El-Samad whole genome screen for "noise mutants" finds genes that increase or decrease noise when KO'd
Gurfield: works in San Diego County, ~size of CT state, 3M people, various ecosystems (desert, ocean, mountain), on Pacific flyway
KC: enrichment cultures with growing Archaea do produce N2O
  Ah I would have loved to have met you! Was only down at  for the day yesterday, drove back to Davis early eve
Sam Miller (U Washington): Using bacteria to probe innate immune diversity  (via )
  Hmmm, not sure about that, I don't think so.  had signs everywhere saying "no photos or video".
KC: archaeal amoA genes show shallow and deep water ecotypes
Antimic peptides in embryogenesis, correlate w microbiota
All disasters start and end locally! “: Next up: All Disasters are local, One Health in our Backyards Nikos Gurfield 
Microbiome analysis suggests cure for chronic sinus condition  ^js
Microbiome analysis suggests cure for chronic sinus condition  ^js
 H El-Samad noise is under selection and is regulated. S c DIG1 affects noise for Ste12 targets
Sam Miller (U Washington): Using bacteria to probe innate immune diversity  
Over express antimic pep changes microbiome. Does it change phenotype? Sympatric speciation? Pretty big leap... 
KC: N2O fractionation produced from Amm ox bact is depleted compared to what is observed in nature 
Next up: All Disasters are local, One Health in our Backyards Nikos Gurfield 
 H El-Samad shout out to Max Delbruck for pointing out cell to cell variability in 1945: phage burst size
Totally wish I was at  :-( pretty please post links for talks that are recorded! 
SM hypothesizes our management of acute pathogenic diseases (antibiotics) may have brought about chronic inflammatory diseases
Nikos Gurfield now on "One Health in our backyard" 
Microbiota controlled by antimic peptides. Ident several from hydra. Taxon specific 
 okay my surprise this morning. There is a viral STD plaguing Beluga Whales. Perhaps Sea World will start a safe whale sex program.
KC: 15N and 18O in N2O, site preference of the fractionation.
Well, this made my day: A Master Of The Microbiome And His Quest To Re-Publish His Father's Papers  
I see  and others tweeting  RT today: Interrogating genomes, 100 years of phage, lunch and then some bioenergy talks
 Hana El-Samad up now on molecular fluctuation (noise)
Yesterday morning's session had nanoSIMS as a common thread, today it's isotopic fractionation. 
Hydra actively controls microbiome species specific. Microbiome recapitulates phylogeny. Plos bio paper 
Thomas Bosch immune system evolved to control microbiome. Path the exception. Uses hydra as model 
KC: focusing on N2O production in the ocean from both denitrifiers and nitrifiers, relative contributions of each 
": Lipkin: now showing clips from Contagion :) " brilliant!!
Heh RT   the movie contagion would have been much less scary if they used the comic sans font
KC: cycling of N2O. It's highest in the atmosphere now than ever measured. Thought to be due to human N2 fixation increases.
Fantastic talk by Nicole King!! Eye opening full of awesomeness
RT   R Brem on avg. N crassa from Louisiana increase N catabolism. Associate expn phenotype with NMR6 alleles
 the movie contagion would have been much less scary if they used the comic sans font
Lipkin: now showing clips from Contagion :) 
Produced by related group of bacteroidetes. Try to find receptor
Getting more  cards for booth 310. Stop by, fill out a quick NGS survey & get yours 
Lipkin: seals can also be intermediates for avian/human flu viruses (similar to pigs, lung cells can bind both) 
Lipkin: Industrial food production can also lead to new viruses, animals lack UV light, low vitamin D 
BW: looking at the effects of heating atmosphere through the lens of nitrate 
Colony formation in S. rosetta regulated by associated bacterium. Bacteroidetes algoriphagus. RIF-1 sulfonolipid signal awesome!
 R Brem: apply analysis to environmental N crassa populations by meta transcriptomes (?)
Seeing all of  's  tweets makes that ASM student membership seem very enticing
 shout out to neurospora project. Not just yeast but filamentous fungi too, nice Dr Brem :)
BW: what controls their relative importance? Organic matter composition. 
BW: anammox and regular denitrification are important to different levels in different places 
 R Brem examine branch lengths for promoters. S p elevated membrane protein expn is from pos selection, not relaxation of selection
Conserved mechanisms in interactions. Looking for models
NK: ubiquity of interactions between modern animals & bacteria hint at a really ancient influence in animal origins 
BW: recent work by Strous shows anammox can produce nitrate, but it's still a net nitrate loss 
Lipkin: estimated 1 million viruses left to be discovered; modeling suggests where to look (hotspots). 
 example of membrane protein regulon. S paradoxus stress response expression different fron S cerevisiae.S p is cons intuitively up
Lipkin  major contamination of PCE reagents with Methylbacteria
Collared cell key trait in common ancestor? key prediction
BW: Sargasso Sea bugs are representative of the whole world so they are important to understand. 
NK: could the last common ancestor of all animals been a bacterivore? 
Lipkin: challenges upcoming. 1) analyze big data 2) eliminate contaminants esp methylobacteria 
  when I became a Florida Master Gardener I did not realize it was going to be another ave of microbiology not an escape
BW: pure cultures show that the rate of nitrate utilization is very fast, even at the gene expression level 
 why are pig brains harvested and packaged? Used for puddings in Asia. Mystery solved!
Choanocytes in sponge sim to choanoflagellate, same function. Awesome sponge video from pbs 
 R Brem: Parent vs hybrids allow estimation of cis vs trans contributions to regulon regulatory divergence
BW: when do they get all the nitrate- they almost never see nitrate in the surface water. 
Use flagellum to create current, funnel bacteria into actin collar, engulf in food vacuole  
.  used  to make a transcript (one day citable?) of a seminar livestream.  
RT  Hey  attendees! Come on over for happy hour and a great dinner when you're done today!...
RT  Hey  attendees! Come on over for happy hour and a great dinner when you're done today!...
BW: looks like the surface nitrate utilizes are picoeukaryotes at BATS
Lipkin: not all zoonoses are infections, eg neurologic illness in pork processors in MN from brain removal & aerosolization 
Choanoflagellate unicellular form colonies closest relative to animals, eat bacteria 
BW: flow cytometry separation to figure out which bugs were using it
BW: deep water fractionation of N is consistent at all times, surface fractionation shows nitrate is being used by something 
 listening to Rachel Brem's talk I am jealous of all the tools in yeast. I must get coccidioides to mate!
SM: Toll-like receptor 2 is required to protect host from chemically induced colitis 
 R Brem: Study regulons defined by classical definition or GO terms. Widespread statistical signal for selection found
  we are already experiencing huge problems with fungal plant diseases being vectored by insects in FLorida.
Unapproved statement from Nicole king. First animals bacteriovores
Microbiology Lab of Future Driven Thru Automation. Dameron Hospital speaker on bioMérieux InBooth Knowledge Forum 10.55am booth1301 
Not registered 4 bioMérieux Scientific Symposium 2nite? Stop by booth1301 to sign up!  speaker at this special event!
Myron Levine,winner of the prestigious Hilleman Award, is giving his lecture at 9:15 am PT in room 103 at the convention center ^lg
BW: the Sargasso Sea is where the small guys have really come into their own. Prochlorococcus. 
Lipkin: Lujo virus, 2008, Zambia. Travel agent sick, transmitted to 3 others, all 4 died. New arenavirus, reservoir unknown 
Fungi are the only group of microbes shown to cause extinction (ala Arturo Casadaval quoted by Lipkin) 
"Fungi are the only type of organism that has convincingly shown to cause extinction." 
Hey  attendees! Come on over for happy hour and a great dinner when you're done today! We'd love to see you!
BW: very slow nitrification at BATS- super oligotrophic- corresponds with Amm ox arch kinetics 
 R Brem: use S bayanus x S cerevisiae hybrid. Follow allele-specific (spp-specific) regulation
Nicole king: stromatolites are fossils of bacterial biofilms! 
Lipkin: fungal diseases also very important emerging diseases--white nose, frog deaths, asthma? etc 
SM finds that bacterial sugars can protect the host gut from inflammation 
RobertShafer: deep sequencing on viruses to ask if a single point mutation is relevant 
Ian Lipkin  - pre-SARS Hong Kong had ad campaign "Hong Kong will take your breath away"
BW: so what about oxygen minimum zones (OMZ)? Amm ox bact/arch maybe not obligate aerobes 
Evolution of predation "eat or be eaten" push to multicellular life sim to biofilm response to predation? 
Poorly worded ad in Hong Kong around time of SARS: "Hong Kong will take your breath away"  
 R Brem: look for patterns of independent changes in cis on genes that encode complexes: evidence for selection
Good morning ! Follow us to find out what has to offer today. And head on to booth 1015 to chat to our colleagues.
 cool, her work is interesting! Please livetweet her talk, then Storify it. 
 brem's work is elegant. Looking for selection in these gene networks.
BW: the reason for a lot of nitrate in the deep ocean is because of low consumption there. Nitrification is slow in the deep ocean
Lipkin: West Nile virus discovery really solidified need for one health paradigm 
Sarkis Mazmanian: gut microbiome composition shifts in inflammatory bowel diseased individuals 
Independent origins of multicellular life lca of animals and fungi unicellular 
that & inter-rectal injection are both glossary adds! “: "Fecal flotation" tests I love  just for the new terms I learn.”
 fact: cats are evil and are trying to kill off sea otters
 nice to see. Great talks in the morning sessions. is a nice meeting
First protist and euk fossils coincides w oxygen in atmosphere
MT : Otters die from T. gondii encephalitis, also those infected 3.7x more likely to be chomped by sharks 
BW: source of nitrate is nitrification. Not all done in deep water- fastest at about 100m where N cycling in general is fastest
Next up Rachel Brem, again on pathway regulation in Fungi
 up next:Rachel Brem on evolution of pathway regulation
Traditional idea of animal evolution 2.5 bill yrs of unicellular life, aquatic bacteria 
BW: nitrate is the dominant form of N in the ocean 
Makes sense -> RT : Loss of wetlands can mean higher levels of parasites moving from land to sea; less filtering 
BW: nitrate pool in deep water is separated from that of the surface.
  hopefully this guy I found in bookstore yesterday is not guilty of transmission
Transition to multicellularity, environment, phagotrophy,interspecies competition
Johnson: transcription networks aren't finished products, they are diversifying and adapting rapidly 
Only cats shed Toxoplasma locusts = disease in many other animals (including people) 
BW: looking at the N cycle "through the lens of nitrate" 
 transcription circuits are not finished products. Take home- may not be adaptive but necessary to make things work
Johnson: Circuit intercalation preserves ancestral logic while adding novelty 
RT : Loss of wetlands can mean higher levels of parasites moving from land to sea; less filtering  
 is trending yet again, and this time on an active Monday morning!
Nicole king berkeley orig and evolution of interaction 
Johnson: regulators and logic of circuit may be conserved but network of genes regulated in different fungi species varies widely
Theodore White "hsp90 and calcineurin inhibitors turn fungalstatic into fungalcidal" does this change resistance development rate?
RT : Loss of wetlands can mean higher levels of parasites moving from land to sea; less filtering 
Loss of wetlands can mean higher levels of parasites moving from land to sea; less filtering 
Sharon Deml presents on Sensititre SLOMYCO ® Plate for Susceptibility Testing of Slowly-Growing, Nontuberculous Mycobacteria now @ .
 A Johnson shows asg as nice example of reconstruction of circuit diversification.
 come to poster 1379 for microbes from 1/2 mile underground! 
Iwasaki: Interrectal injection of LPS in antibiotic treated mice restores virus-specific immune responses 
DJ: dsrAB gives a very reproducible fractionation of 11 per ml, much smaller than expected 
eeewwww! Remote controls: one of the most heavily contaminated items with bacteria in hotel rooms -> 
DJ: enzyme specific fractionation experiments (instead of whole organism) to define pathways. DsrAB 
Wild cats have higher levels of T. gondii (bobcats, mountain lions, feral kitties); feral & bobcats shed the most 
Want to learn about Detection of Group A Streptococcus from Throab Swabs by PCR using Roche and Focus ASRs? Catch Jim Uhl @ 10:45am 
"Fecal flotation" tests to detect T. gondii oocysts. I love just for the new terms I learn.
DJ: when you look at the fractionation of thiosulfate, you get a really big signal 
 the number of cute cat and otter pictures in this talk is off the chart!
 A. Johnson: circuit intercalation a mechanism to rewire while preserving regulatory logic...and providing opportunity for novelty
DJ: loss of fractionation signal using sulfite is due to neglect of thiosulfate 
Pseudoaltero also promotes coral settlement and sea urchin
DJ: sulfite is Penn Station. Everything goes through sulfite.
DJ: ...attribute that to a microorganism. Now we're pushing those limits. 
DJ: for the last 40 years, sulfur fractionation was restrained to a limit, meaning that if you saw anything under that limit... 
Wow Toxoplasma gondii oocysts are tough! Soaking them in bleach doesn't stop them from being infective! 
 Conrad lists many disciplines required to tackle this otter problem, forgets to put 'microbiologists' on the list! 
Reef coral settlement. Internal fertilization release larvae that settle on biofilm, form polyps 
Dr. Elli Theel talks about the identification of yeast using a direct on-plate extraction method today at 10:45am. 
 only 8 genes in trancrption targets conserved. But rewiring conserves function. Is this a product of genome duplication?
Found operon in pseudaltero promotes settlement, not altering biofilm structure. Blast search didn't help 
DJ: their chemostat studies were done with Desulfovibrio vulgaris Hildenborough 
 seems like the solution to this otter problem is to install many, many cat litter boxes!
P Conrad: Dz causing agents on land can affect our oceans
Disease-causing agents on land can affect health of ocean inhabitants 
DJ: this inverse relationship can now be used to look at geochemistry 
 extensive retiring of txn factor targets in fungal lineages, but regulatory logic is retained.
DJ: inverse relationship between sulfate reduction rate and sulfur fractionation. 
Oocysts from cat feces likely source of T. gondii for otters 
Cytophaga lytica, pseudoalteromonas luteoviolacea pro settlement from ocean, atcc strain doesn't  
DJ: experimental sulfur fractionation data comes from batch cultures, now chemostats, both pure and enrichments. 
Iwasaki shows using mice with modified immune systems that autophagy is important to virus detection 
Toxoplasma infections makes sea otters more likely to be killed by sharks 
Otters die from not only T. gondii encephalitis, but those infected also 3.7x more likely to be chomped by sharks 
RT : DJ: each different sulfur metabolism has a distinct fractionation when minor isotopes are included. 
Loving the cute pictures! MT  kittens, otters a beautiful ocean vista and a nasty Protozoa 
Key to settlement diverse biofilm on surface, not a particular bacterium 
Toxoplasma gondii infection of sea otters - can cause protozoal encephalitis. Poor sea otters! 
Sea otters endangered by toxoplasma gondii encephalitis, federally threatened species 
 kittens, otters a beautiful ocean vista and a nasty Protozoa: Toxoplasma gondii infecting brains of otters - Conrad
Collect marine biofouling polychaete by putting PVC panel in water.
DJ: the whole goal is getting experimental evaluation of fractionation so the geochemical record can be interpreted. 
DJ: each different sulfur metabolism has a distinct fractionation when minor isotopes are included. 
Bacteria were there first, larvae had tides with it from the beginning
 Sandy Johnson:We use "circuit" in several different senses
 coccidioides on the tree, ability to infect humans has evolved many times on diff lineages, mostly regulatory?
Clock of earth history- nice infographic! 
Iwasaki discusses the various immune system sensors that detect viruses at 
Intertidal banding selective settlement of larvae. Soluble and adsorbed factors 
My poster is up, board 1399. Conveniently close to the restrooms    disease
 Sitting up front due to the many instances of audio fail yesterday. Sandy Johnson talk starts w/audience clamor to move the mic.
DH: sulfur isotope fractionation is huge and can be used to track sulfur geochemistry 
Michael hadfield "lowest chordates" ? Marine biofilms interact w invert larvae 
First up: Patricia Conrad, Division Z lecturer on Otters, Oocysts and Oceans 
Unseen forces microbes shape animal biology 
  integrate focus on humans, animals and the environment - one health
David Johnson from Harvard on sulfate reducers 
Hear Brian Lucas talk about Improved Pyrazinamide Broth Susceptibility Testing for Mycobacterium Tuberculosis Complex at 10:45am. 
RT  : : "If you care about human health, you have to care about animal health and environmental health" 
And by happy coincidence, the first talk starts with a picture of a nebula  
: "If you care about human health, you have to care about animal health and environmental health"  
Iwasaki is this year's winner of the Eli Lilly and Co. Research Award. Congrats! 
In the Cmte on Undergraduate Education Board mtg. A lot accomplished and now we'll see what new ideas we can come up with! 
Flitting between One Health and Microbes Shape Animal Biology sessions this morning.  
Listen to Bess Ward, award winner, at 8:45 am PT in the Esplanade Ballroom 305/307 give her talk about nitrate consumption ^lg
this one is my 1st stop today as well. “ first session today, One Health convened by  Room 103”
And continuing the star wars theme from the opening night, we have Christopher Lee talking about phenotype sequencing later on
My poster is up- 2092 way in the back. I'll be presenting from 1:00-2:45pm 
Back for the second full day at . I'll spend my morning watching the session "Microbes Trigger and Shape Immunity."
Interrogating genome session at  about to get started, first up Alexander Johnson on transcription circuit evolution
Ahhh,  seats my chiropractor Thanks you from the bottom of his wallet!
 first session today, One Health convened by Room 103
Contamination-free pumping results in improved efficiency. Take a look at some products at the  booth 1015
Are you in San Francisco? Come visit DNASTAR at  . We are in booth 1518, offering live Lasergene demos from 10:45 - 4:00.
I need Hermione Granger's time-turner to be in multiple places at once to see multiple talks at 
Day 9 of marathon conference trip & I have hit the wall. Today will mostly be fuelled by caffeine and microbial community spirit!
If you're at  this week don't miss the Idaho Tech Booth #514! Check out the FilmArray, new targets, and collect the next bug magnet!
Scott Cunningham discusses Identification of Gram-Negative and Gram-Positive Aerobic Bacteria at  today at 10:45am.
: Note to organizers: scheduling business mtgs before buses start ain't good for attendance  ” seriously?
Akiko Iwasaki, winner of the 2012 Eli Lilly Award, is hosting a lecture at 8:15 am PT in the Convention Center, room 104  ^lg
Renewing ASM m'ship yesterday proved gd plan when got back2hotel room last night2find my pjs missing.Free tshirt came in useful! 
Here's an idea. A TV remote coliform containment condom: study in hotel rooms. Stay away from TV remote!
Looking forward to following , thanks to everyone tweeting
 Will you have a report from American Soc for Microbiology meeting this week. 
Getting set to see several microTweeps today:  and. Then former students. Then obsess about talk
On my way over to  for more talks this morning 
No paranoia, we have immune system. RT study on bacteria in hotel rooms. Stay away from TV remote!
RT : Attending  today? Come visit us at the Wiley-Blackwell Stand 724 for the Pathogens and Disease event from 11am
New blog post- Experiencing the culture & climate of ASM, Day 1 on our website:   
Dr. Joanne Bartkus, MN Public Health Lab Director, is the chair elect (2012-2013) for Division Y. Congratulations to Joanne! 
Division Y Business Meeting. Go Public Health!  
Are you at ? bring this flier  to the Daigger booth and they will donate $5 to Seeding Labs
Last day at  ! Come see Siemens’ broad microbiology testing portfolio  701. 
Note to organizers: scheduling business mtgs before buses start ain't good for attendance  
Stop by booth 723 at  for a free sample of our alternative to SuperScript II – equal performance, ½ the price!
Lots of APHL staff are attending! RT : Excited to be attending this year's  General Meeting 
Another busy day ahead at . Interrogating genomes, 100 years of phage, lunch with  and then some bioenergy talks
Just in time for  study on bacteria in hotel rooms. Stay away from that TV remote! 
Attending  today? Come visit us at the Wiley-Blackwell Stand 724 for the Pathogens and Disease event from 11am
 Pathogens and Disease meet-the-editor event today 11-1, Wiley-Blackwell Stand 724. Free refreshments! 
Video of ASM-Live w/ me,K. Kirsch & S. Malloy on "Microbial Analysis of Environmental Surfaces in Hotel Rooms" 
 2.0 is here! The next generation of handheld, automated counting   Booth 1523
 just received a copy of "microbes and evolution", for those who couldnt attend 
 hey, couldn't come to  so at work now. It's 9:55am GMT here now. Hope you guys are enjoying yourselves ;)
MT : Uploaded vid of my talk on  from-http://ow.ly/bDUu7-not my best but still feel like I should share
 We really hope you'll have the time  to stop by our booth - 1625 and let us explain about . Can't wait to meet U :)
 Congrats on a great talk . Love to meet U. UR invited to our booth 1625-let us show U  tool




Abt 50% correct GO annot, more nearly right. Not bad. Compares very well w machine annot 
 loved your  talk, very inspiring. Makes me even more committed to  as a practicing scientist
 Abundance or Diversity? Does Ross wager to imply that 100X more microbes in water is good or bad? 
Will come up again Tues 3pm Rm 305 MT  :public respects scientists but 2/3 cant name living scientist. 
Very impressed by the diversity of attendees at ASM this year. For example...  
 rattle snake oral flora is aerobic and anaerobic - horrible pic from rattle snake bite!
Some protein families appear to be better represented in the diseased state, many of which appear to be involved in stress response 
: Ross says the bottled water she has surveyed has 100x as many microbes as tap water ” may change my shopping habits
Think about annotation process when writing papers...good idea, tough to implement 
" life lesson: don't ever get a monkey as a pet...or a Komodo dragon." or a rattlesnake!
Friends of ABG at  link up at Foleys Irish Pub on O'Farrell and Powell at 9 on Monday (243 O'Farrell Street, San Francisco)
.: public names LIVING scientists like Eisnstein or Sagan (both dead) or Bill Gates or Dr Oz (Not scientists) 
 life lesson: don't ever get a monkey as a pet...or a Komodo dragon.
Li: found that some genera are significantly impacted by Chron's disease. It looks like the disease suppresses them 
Ross says the bottled water she has surveyed has 100x as many microbes as tap water 
: When students challenge annotations in CACAO, they can steal points from each other
 : the public respects scientists and wants to hear from them! But 2/3 can't name a living scientist. 
Student teams compete. Choose any protein they want. 
Ross: shows slide with lots of gunk and microbes and says she wants to show us pic of what we are drinking from tap 
.: NIH director Francis Collins is an evangel Christian but has no prob w/evolution--can connect w/religious audiences
Li: 6 monozygotic twin pairs exhibiting healthy/diseased conditions. Conducted quantitative community proteomics on stool samples
 McIntosh on CACAO - community assessment of community annotations with ontologies. Crowdsourced annotations!
Now up at  Kimberly Ross discussing microbes in municipal drinking water
: Gene Ontology = controlled vocabulary + useful relationships between terms.
shd have said are /often/ given to cats with cat bites 
Hierarchical label in gene ontology helps w searching 
Li: Crohn's disease is a poorly understood, complex disease of the human gut 
Shd we give "prophylactic" antibs to ppl who present early with bite wounds?Are given to cats with cat bites in UK. 
+1 RT : Dear scientists, Fewer words per slide, please. Love, your audience. 
: Who is going to the  Poster Hall Networking Reception tonight in Hall C from 5:30pm-6:30pm?” I'm considering it.
Couple annotation to teaching. Students make GO annot w CACAO
Curation rate limiting step  crowdsourcing
: BMcIntosh: dealing with lists of genes->databases->synthesis. Depends on the quality/quant of what's in the db
Lists of genes, figure out new functions. Ask a biochemist? Prob not. Curation 
Hey  , are there grad students/nice people who want to pal around downtown San Francisco? Dinner/drinks or something?
Competitive annotation of gene function  cool!
Humeida: of 3 sequenced symbiont genomes (are there more? Maybe I misunderstood), surprisingly sparse for secondary metabolism. 
RT : Photo shown of testicular bite received while owner teaching pit bull to bite. Serves him right! 
RT : Sanko says he refers to rare microbes as "the less than 1%" 
Humeida: sponges filter 50,000L seawater per day (!?); efflux sterile. Contain bewildering variety of associated microbes. 
Pasteurella in cat bites>dog bites. P. multocida in cat bites but P. canis more common in dog bites 
Photo shown of testicular bite received while owner teaching pit bull to bite. Serves him right! 
Using cDNA from transcripts can help identify rare organisms that are active in a community. 
Controversies in transplantation session time 
Some very grim pictures being shown in bite talk! 
Hentschel-Humeida: sponge microbiome review of findings. Developed single-cell whole genome amplification method.
Chandler: some microbes also eavesdrop on competitors QS signals - think gives adv to microbes just below the critical conc for QS
  thinks "science denial isn't anti science." Wrong, but answer is complex. Denial shows understanding of sci is weak.
: BBaker used Velvet-Oasis for assembly
 : Brenley McIntosh talk on Community Annotation that I've been tweet-promoting up next.
Only deep-sea nitrite organizers found were Nitrospirae. Low abundance genes but high transcripts. Rare but active. 
BB: called these new bugs DONN- they and Thaumarchaea were most dominant at depth based on mRNA (I think I got that last part).
Cat bites mostly on hands. Dog bites distributed more widely over human body. 
Sanko says he refers to rare microbes as "the less than 1%"
Cats and dogs bite different body parts preferentially. No cat bites to head and feet but lots of dog bites 
Cool - John Sanko finding halothermophilic microbes in flue gas desulfurization system slurries 
BB: however, Nitrospira were very low in rRNA 0.3%. No other known nitrite oxidizer rRNAs tho 
:B Baker () analysis of metatxome... I'm wondering about robustness of reconstructions in general to annotation quality
Zoonoses session def put me off travelling and pet ownership
BB: good homology with Nitrospira nitrite reductases, also other transcripts match Nitrospira 
  on stage, talking abt surveys: those that couch their qns as non-contradictory to religion get higher response. Sigh.
Chandler: QS important for Antimicrobial synthesis and competition in soil microbes - cool competition assays - FIGHT! 
Oh: skin disease lowers bacterial diversity 
Ammonia-oxidizing Archaens can make up 40% of the cells in the deep sea. 
BB: focusing on resolving which bugs are responsible for nitrite oxidation. 
BB: marine group II Euryarchaea amino acid transport transcripts were high. 
Baker: highly active organisms such as Alteromonas are present at low coverage 
Great day at  we love meeting our customers & hearing about your research! Stop by booth 1616 tomorrow & say hi!
Bite wounds gen infected with oral flora of biting animal (which may reflect what that animal eats) 
Learning about the microbiology of animal bite wounds at 
BB: Alteromonas was highly active but low DNA abundance
clin micro rev (2011) 24:231-246 makes up some of data in bite talk
Brett Baker from U Michigan talking about de novo assembly in transcripts done w/#Hiseq on deep ocean vent communities
BB: assembled transcripts into contigs, identified genes, to look at geochemical impacts of rare microorganisms 
Julia Oh: how primary immunity shapes bacterial communities
Setting up camp for a few talks, so I'm adding notes to the FriendFeed again: 
BB: hydrothermal plumes introduce a lot of reduced material into the water column 
Last jump metatxomics of deep sea gulf of CA 
Excellent talk from Sally Cutler :) Now onto animal bites... 
Next is  discussing de novo assembly of transcriptomes from deep sea hydrothermal plumes. 
 Up next: Brett Baker on de novo assembly of deep sea transcripts
If you have Latina students, they may be intetested in this dual language science website from Puerto Rico 
S Cutler: Exotic pets come in & out of fashion. Me: pet rats are always fashionable! 
 Hear that  MT  trouble with Bordetella in Boulder b/c lots of parents don't vaccinate kids
Heath-Heckman: E. scolopes squid, V. fischeri bacterial symbiont. Light from bacteria regulates host cryptochrome transcription.
 Amblyomma - highly aggressive hard ticks that run after you for up to 30 meters!! Jeez '
mT : Great talk from  on science denial, will the issue of mmr vax ever go away?  
Alegado: Choanoflagellate closest to multicellular animals; assoc. bacterial sphingolipid -> multicellular behavior in fM range!
mT  Tara taking the audience on a fascinating journey thru' antivax & other assorted pseudoscience. Great stuff!
S Cutler now talking about ticks that will chase you for 30m to get a bite...  
mT  Tara [Smith  ] chronicling the devastating effect of HIV denialism & vaccine 'skepticism'.
mT   lamenting sad state of science education in US, esp. the shrinkage of science coverage in news media
JH: Looks like some microbes activate immune system, leading to increased morality. 
RT   opening the session on Science Denial and the Internet [rubs palms in anticipation!!] 
Some1 was unlucky enough 2 get lepto after giving CPR 2 some1 who drowned in a waterfall (well done the medic who got that history) 
mT  Emmanuel Roilides (Aristotle U, Greece)invasive candidiasis in NICU ; neonates highest incidence :(
I'm in! RT : Who is going to the Poster Hall Networking Reception tonight in Hall C from 5:30pm-6:30pm?
Aspirin and ibuprofen protect caterpillars against Bt 
JH notes that willow diet reduces catapiller death. Willow contains antiinflammatories 
 Leticia C-M: English is a monster to her students until she drops them in the US.
Very interesting talk of thomas bosch about tumors in hydra, at
mT  all cases candidemia require treatment; morbidity, mortality similar to bacteremia by staph aureus - Tom Walsh
S Culter: Schistosomiasis infections rel high among travellers after swimming/kayaking/rafting on the Nile in Uganda 
Willow trees suppress effect, produce aspirin! 
 great talk by Prof Sally Cutler on unwanted tropical souvenirs - Zoonoses and Travel...'potential transmission via bush meat' 😰
Inflammatory response kills caterpillars 
 Robyn Wilson, on biases that come in to play when processing information. Egalitarians & communitarians see low risk in vax.
Hear that Jenny McCarthy? MT  lots of trouble with Bordetella in Boulder b/c lots of parents don't vaccinate kids
Enterococcus toxic when directly injected into haemolymph
What talks are people most excited for on Monday 
I'll be there RT : Who is going to the Poster Hall Networking Reception tonight in Hall C from 5:30pm-6:30pm?
Just ran into Kelly Hughes, who taught the only official microbiology course I ever took, back in 2003 at Cold Spring Harbor 
"Availability" of examples... Do you know someone with autism vs. do you know someone with polio. This one is Huge. 
Model bt breaks epi, gut microbes invade 
 Leticia C-M: collaborate with a group in Florence because I always wanted to go there... B. subtilis eftA affects calcite formation
Abstract cards = great & glad to learn about their service MT  creative marketing is a key! 
Handelsman: Bt killing of Lepidoptera requires the resident gut bacteria! 
Who is going to the  Poster Hall Networking Reception tonight in Hall C from 5:30pm-6:30pm?
RT : RT : Overheard today at "I got norovirus. It was awesome!" ^js (oh  )
Hernandez says they have lots of trouble with Bordetella in Boulder area b/c lots of parents don't vaccinate their kids 
S Cutler currently putting me off exotic holidays! Rodent infestations, snakes, fleas, mosquitos... 
Zwittermicin effect not based on just killing gut microbes. Bt activity dependent on gut microbes -enterobacter 
JH knocked down community abundance and found that larval mortality dropped. Pathogen susceptibility depends on gut microbes
Hernandez now discussion PhD thesis work of Jordan Peccia who is speaking later  
 is continuing education for non-microbial biologists.
Tremulacin attacks gut wall. B. Cereus zwittermicin a synergizes, also w tobacco hookworm 
prob still better than home though RT : Kirsch: hotel room surfaces can appear clean but may be very very dirty
 Jayant, Adam and the entire team busy  - long line even at lunch time to get networking pass
Didn't attend for same reason  RT  I had to leave out of fear I could never stay at a hotel again 
Aeroplane travel - it's not just us that's travelling on the aeroplanes, arthropods are as well! 
Elizabeth Heath heckman: squid can sense light from bacteria and modulate expression of cry1 
 In assessing & dealing w risk & perceptions, how to combat the effect of misinformations by bloviating celebrity know-nothings? RW
Dr. Castillas Martinez engages "invisible students" -- those that dont qualify for typical university programs -- in her research. 
JH finds that diet influences pathogen-infected catapiller mortally rates. Suggests commensals play a role 
RT RT : JA: built environment easier to control than nature, easier to replicate. 
RT RT JA: built environment easier to control than nature, easier to replicate. 
and mops, rags used by cleaning teams! MT  TV remote & main light switch are two of the "dirtier" spots in hotel rooms
Aspen, willow change effect of bt. Aspen produce phenolics synergies w bt 
People driven by emotional value of loss, not likelihood, in assessing risk  
Leptospirosis: now seeing recurrent epidemics. ?assoc with climatic change/change of land use/growth of urban slums? 
Firmicutes enterococci, staph, proteos enterobacter, pantoea. Diet effects on flora 
Catapillers have an orally acquired pathogen, commentary organisms, and dietary variation 
Dr. Casillas Martinez recognized for being an excellent mentor of hispanic women from low income homes. 
JH: Bacillus thuringiensis kill insects with crystal toxin 
 RW taking special example of vaccines to understand the intersect b/w risk perception & decision making; currently defining 'risk'
Bt pathogen for Lepidoptera, tobacco hornworm and gypsy moth.
Wait, what's the point of listening to marketers if we can't trick people into making good decisions???  
JH uses the catapiller gut as a model to study host-gut community interactions. 
correction re: last tweet -- speaker was Christopher Rath. Apologies!
Sticking with the gut theme, next up Jo Handelsman and the caterpillar gut microbiome & disease 
 Robyn Wilson, social scientist from Ohio State, on pblc perception of risks asso. w emerging diseases: uh-oh,?
Talk about microbiome of caterpillar guts! 
Word. RT : Dear scientists, Fewer words per slide, please. Love, your audience. 
S Cutler:Are we better thinking of zoonotic infections as multi-host pathogens rather than human infections acquired from animals?
Did they look at that?! “  things you do not want to know. Sleep on the floor  
Mark Hernandez using methods to introduce known amount of microbes into aerosols 
F. Cunningham on the discovery of provitamin A synthesis, Vitamin A deficiency and the creation of Golden Rice 
Nicholas Rath mass spec as a platform for spatial systems biology, re: effects of microbiome on metabolism 
Dear scientists, Fewer words per slide, please. Love, your audience.
Can I just say I want to be Mark Hernandez when I grow up?
Now up at  Mark Hernandez on stability of airborne microbes to master environmental variables
I really love the uploaded discussion videos so far...very informative ;)
Mark Hernandez (UC Boulder): Stability of airborne microbes to master environmental variables. 
Comparing to germ free mice identifies microbiome specific signaling molecules 
 Actually, CACAO=Community Assessment of Community Annotation with Ontologies. Annotation as a non-NCAA intercollegiate sport
 should probably have a warning, "Not for people with acute OCD."
Can then innoculate germ free mice with microbes and compare mass spec profiles from mice with different microbes 
SaPI mobilization more complex than previously thought phage can protect selves from SaPI co-option 
 Brenley McIntosh talking about Community Annotation soon in Room 133. Join CACAO=get letter of support from us for your NSF Career
 Thanks for stopping by my poster, and for the t-shirt! You make my research so much easier. 
Rath uses computational techniques to deconvolute complex mass spec data. Can find small molecules. 
KK: Housekeepers only have about 30 min to clean each hotel room - no time to focus on non-priority areas (light switches, remotes)
Kirsch: sinks, sponges, and mops have a lot of potentially dangerous aerobic bacteria .
Kirsch: hotel room surfaces can appear clean but may be very very dirty  me: sort of like people
Air microbiome: Nicholas Be using deep sequencing/metagenomic analysis to characterize air in public spaces, like mass transit hubs
KK: Headboards and curtain rods were most clean as measured by metagenomics. Sounds like a challenge...
The main light switch in hotel rooms is often coated with coliform bacteria - double yuck. 
 whoops meant APC - aerobic plate count
SaPI particles different from normal phage particles, diff size, SaPI encoded proteins force packaging of SaPI not phage 
Rath uses model commentary bacteria to study nutrient exchange
KK: TV remote & main light switch are two of the "dirtier" spots in hotel rooms, as measured through Coliform bacteria 
Methanosarcina implicated in mass extinctions at Permian-Triassic boundary (with a little help of genes from Clostridium) 
 kirsch hotel app results: head board & curtain rod least cfu, maids cart mop and sponge the highest cfu
Rath uses image mass spec to model metabolic exchange in our guts 
 food safety FTW! Kirsch reviews HACCP as solution for inconsistent hotel cleaning practices
Kirsch says HAACP protocols were used in testing hotel rooms
Katie Kirsch : Getting a food-born illness in zero gravity would not be so great. SPACE PUKE!!!!!!! 
I had to leave out of fear I could never stay at a hotel again 
Back in the bowels, first with talk on Bifidobacterium dentium production of GABA and now imaging mass spec & gut systems biology 
Temperate phage role in transmitting other elements like SaPIs, defective phages with toxins. Super antigens! 
Hoping Kirsch focused on sampling hotels for microbes outside of San Francisco 
Cool example of how phylogenetic analysis of Methanosarcina tested a hypothesis that originated from physical science 
Kirsch: SARS and bedbugs getting mentioned as calling attention to hotel uncleanliness 
Watching Rath discuss host microbe metabolic exchange 
RT  John Fuerst: Gemmata: bacteria(! Planctomycetes) with a nucleus-like internal structure, cell division.
Now up Katie Kirsch discussing microbial surveys of hotel Rooms
Lots of SaPIs since I left the field. Staph genetics is crazy 
A microbial analysis of environmental surfaces in hotel rooms - gross! 
Next up in   session: Katie Kirsch talking about the microbiology of hotel rooms - disturbed yet intrigued!
Factoid for : even as you speak, Pakistani Taliban bans polio vaccination campaign  Religious madness
Scott Ouellette on distorted chlamydia shapes in penicillin analog treated cells 
Talk by Altaira Dearborn from u Alabama birmingham 
Jump to new session, talk about staph path islands and helper phage 
RT  John Fuerst: Gemmata: bacteria(! Planctomycetes) with a nucleus-like internal structure, cell division.
Bifido. dentium is the primary producer of GABA in the upper ilium
 I had to weigh b/w  &  to decide which talk to attend.  Sorry, Dr. Eisen! Tara gave a great talk.
Be: Found shifts in microbial population according to season, in both abundance and composition 
Come to the convention center today at 4 pm PT in room 133 to hear an  award winner, Lilliam Casillas‐Martinez, give her lecture.^lg
If you have a poster at  then you ALREADY have FREE Networking cards. Come pick them up right outside hall C.
Be: Collected aerosol material from public facilities over 1 yr, used PE Illumina seq and mapped reads to taxonomic IDs 
That's it , I'm including  in all future  tweets. I know she loves you work.
 James Hughes sez media interest's inversely proportional to disease incidence. Sadly, c'est vrai. Spreading FUD hurts public health
Permian-Triassic extiction 252 mya killed 96% of marine life! (including giant sea scorpions) 
Giant viruses 'giruses' can have viruses of their own: 'virophages.' Wow. 
Nicholas Be from Lawrence Livermore: What microbes surround large highly mobile populations, and what forces act on them?
Pick up a free copy of your favorite ASM journal! Here today til 4, Exhibit Hall booth 1308  ^nr
 great audience qn after Tara's talk abt involvement of scientific companies in funding climate change skeptics like Heartland Instt
Yep, that's my PI RT  : "I'm recording this talk & will be posting it later even tho asm will be pissed off"
 can help you figure out how to share your science using social media.  encourages you to get in touch
Eisen: Great perspectives on science and social media. Sharing is not just caring, it's good science. 
 : "I'm recording this talk and will be posting it later even tho asm will be pissed off"
 Sloan foundation is funding microBEnet and microBE projects
Reminder, we have a public friendfeed going for archiving notes: 
I'm bouncing around concurrent sessions for the next few talks, so I'll be less active, esp on FF. 
ASM police crash through the door and throw  to the ground. 
 we can help u how to share ur science more broadly Amen
Rohwer: black reef =algal microbial mat crawling along bottom; microbial mediated iron cycling. Bacteria = shock troops. 
 John Fuerst: Gemmata: bacteria(! Planctomycetes) with a nucleus-like internal structure, cell division.
Not tweeting his own  talk, but "I am recording slides and audio of this talk, which I'll post on the web later" - 
"Tteach someone to share and you help them (and everyone) for a lifetime"  at   session
Phew, finished presenting my poster at ! A rarity when I get to talk science in more than 1 language. So many cool people!
Giruses should provide lots of opportunity for grad students to drink beer and debate whether viruses are alive  
JE drawing laughs for mentioning he's recording his talk and might be pissinh off ASM 
 share something for someone and you help them for a day. Teach them to share and you help them and everyone for a lifetime
Great talk from  on science denial, will the issue of mmr vaccinations ever go away? 
JE wants MORE TWITTER FEEDS. Give him what he wants!
  plugs twitter for microBEnet project "I personally think twitter is incredibly valuable for keeping up w science"
 social media are powerful tools for sharing science. Already there are at least 65 microbiology related blogs
My new goal is to get the work "Eukaryotes" into  's word cloud figures when he gives talks  
Terrible anti vax rapper, someone is wrong on internet 
 Tara, you're making me simultaneously nod in agreement & shake my head in disbelief & disgust. Result? A circular motion of my head
Rohwer now talking about black reefs - iron from shipwrecks causes algal overgrowth on reef, killing corals 
Scientists should use social media as a powerful tool for sharing scientific information, e.g. Facebook groups to build communities
microBEnet: : keeping up w/ literature can be burdensome, highlights Mendeley '(like EndNote w/ Facebook mixed in') 
Bloody twitter autocorrect changes tweeps to twerps! 
microBEnet : Adding value by talking with one another. Hear that,
 impossible to keep up with scientific publications. mendeley can help. It is Endnote + Facebook= good resource.
 Tara taking the audience on a fascinating journey thru' antivax & other assorted pseudoscience craziness. Great stuff!
Eisen: Using Mendeley to create social references of literature: EndNote meets FaceBook 
 shout out to the twerps, fecal transplants, old post and Grey's anatomy 
Rohwer: cites Dave Zawada's work showing coral GFP fluorescence changes when coral is stressed, incl when encroached by algae 
Yep - now all we need is more Sake RT : talk is basically a tweet-up with a lot of crashers. 
Sad but true RT : Scientists can't avoid Internet, will become irrelevant  microbe world aetiology
Scientists can't avoid Internet, will become irrelevant microbe world aetiology
The talk by  is basically a tweet-up with a lot of crashers.  
Sending chickenpox thru the mail. Isn't that bioterrorism? 
 Jonathan Eisen, my social media guru, is now speaking about microBEnet
Rohwer: interfaces btw corals and algae have their own unique bacterial community, incl Vibrios 
Apparently, if you are , you can get paid to blog and tweet. Where do I sign up? ;) 
Low vaccination rates in parts of Oregon, chicken pox parties
 I think  gets paid per tweet! This explains so much!!” like !
PBCV-1 encodes 11 tRNAs and is doing transcription just 10min post infection. 
NVIC and IMCV stealth anti-vaxxers. Deny herd immunity wtf?
Rohwer: corals win against CCA in uninhabited areas; near people turf algae wins over coral. 
Girus PBCV-1 infects Chlorella that are usually symbiotic in Paramecium bursaria 
Jenny McCarthy body count. Push back! autism correlates with Jenny McCarthy fame 
If you collaborate with JA and you're in the room, you're getting pointed out. 
Rohwer: degraded, overfished reef favors lots more microbes incl pathogens bc more algae exuding DOC 
Great poster session. Now for a talk on fungal pathogens before heading to a Staph talk. 
Eisen notes that DNA based investigations have been readily adopted among researchers of some communities, but not others.
 stop telling me to find a seat in packed sessions when I'm session-hopping!!
Vaccines -bill maher and Mayim bialik. Bill maher denies germ theory!
 Tara chronicling the devastating effect of HIV denialism & vaccine 'skepticism'.
Rohwer: Corals & crustose coraline algae are rebar and cement of reef 
Love that I can follow "Soundbites to Superbugs" session while also learning about campy - thanks to all tweeting at 
Jon Eisen: new approaches to studying microbes in the built environment are culture independent, incl NGS 
Lots of people died because of Christine maggiore and thabo mbeke including her and her daughter, website still alive 
Eisen jokes: Showing the movie Contagion on an airplane probably isn't the best idea 
RT JA: built environment easier to control than nature, easier to replicate. 
"Viruses are the biggest source of genes on the planet." 
 Glad you're watching that. I've had a hard time selecting among the great talks this afternoon! 
 Tara Smith (  ) lamenting sad state of science education in US, esp. the shrinkage of science coverage in news media
Rohwer: crawling holobionts and microbial mats in corals 
Tara is using HIV, vaccine denial as examples (not creationism?)
JA: built environment easier to control than nature, easier to replicate. 
Talk: Crawling holobionts and microbial mats: Forest Rohwer (SDSU).  
I wouldn't be surprised if  tweeted while giving his talk. 
Free t-shirt, cookie and coffee! Stand 724, meet the editor of MicrobiologyOpen, 
Division W chair asks for more "CUE-like" abstracts submitted to main meeting. "Consider submitting to both next year." 
Turn to Internet for science info, or stumbled upon. Amplifies misinfo.
 Look forward to Vic Di Rita's talk - division B lectures
"When Good Bugs Go Bad" Forest Rohwer just handed me a pair of glasses that say "coralandphage.org 10 ^31 viruses can't be wrong"
Science coverage in news sucks (no kidding) 
Jonathan Eisen () - The Microbiology of the Built Environment Network  
  opening the session on Science Denial and the Internet [rubs palms in anticipation!!]
Campy talk followed by the zoonoses session this afternoon for me - my fav topics :) 
Jonathan encourages more people to tweet , including people that "don't know what twitter is"
This is going to be a highly redundant session! 
Division W chair started the session with a shout out to CUE 
On to  talk on built environment micro. I can see at least four other tweeps    
Having a blast at ! Can't wait to hear present tonight during the bioMérieux Scientific Symposium at 6pm
Finally managed to get iPad functionality. Blame the comp illiterate user. Ready to live tweet from Dr. Tara Smith's session 
Afternoon session with  discussing genome streamlining. Thanks David! 
Good point, Tuesday not tomorrow! Days are a muddle right now RT   Tuesday surely
mmmm.... “: Also, tweetup in poster hall at 5:30? Supposed to be beer somewhere there... 
Good to meet  for lunch today - if you are following then why not check out the fourth  tomorrow!
ditto “: Jumping between sessions this afternoon. First up, microbiology of the built environment w/ 
In the ASM Div W mtg at . Elizabeth . Emmert reporting out on working document of ASM Teaching Lab Biosafety Guidelines.
Chk out bigger than life  pics at the booth in row 1600 at . Thx for ur photo releases!
I just saw your name on board as I was walking by. Congratulations. “: I has been elected  
Jumping between sessions this afternoon. First up, microbiology of the built environment with  
Blog: "Fecal Matter Hiding in Hotel Rooms" by Jenniffier M. Mahand, M.D.  - Presented at .
 come see PortEco and EcoCyc at booth 1623. We are next to the very popular labguru booth
 Free t-shirt, cookie and coffee!!! MicrobiologyOpen journal, meet the editor now!!!
These great  sessions should inspire you to vote for. We want your ideas! More info: ^kl
Day isn't over yet  ppl! Come meet us at booth 1625. Learn about   tool & get cool gifts. See U there.
I may have stolen a bottle of sake from that tasting. I may have stolen two. For science. 
 From Rob Edwards:Meet at Foleys Irish Pub on O'Farrell and Powell at 9-ish on Mon Please invite everyone, and fb and/or tweet it.
Meet the author of our Culture Dish blog!  will be at booth 1616 at  to answer your technical questions!
L. Barth Reller, award winner, is hosting an interactive quiz about.Come to the Esplanade Ballroom 304 at 3 pm PT! ^lg
 Why you should make div M your primary or secondary (aside from phage being awesome): more student awards than most others.
Hiro Imachi has a brilliant solution to continuous-flow bioreactors: dripping sterile seawater thru sponges in an anaerobic tube
Prepping for  session I am chairing "The Great Indoors: Recent Advances in the Ecology of Built Environments"
The most stable zero-oxygen conditions - demo the Bugbox at booth 1534. 
Also, tweetup in poster hall at 5:30? Supposed to be beer somewhere there... 
Recovered from sake? (or not? tha's ok too!) Come to "Soundbites to superbugs" 3PM 306 Esplanade ballrm 306 
 Meet the Editor of MicrobiolobyOpen now! Free t-shirts stand #724
 Congrats to Men-Lun Hsieh from Hinton lab for NEB award. Talking about T4 txn and sigma appropriation
New MIDI PLFA method more info at booth no 1036 
repeat after me  presenters: NO MORE COMIC SANS!!
To everyone at , come checkout our booth (840) to find out all about Omnigene for simplified microbial DNA & RNA sample collection.
Tweeting might get wild after sake-tasting since many tweeters were in attendance 
Talk: Larry Forney (U Idaho)-- Diversity in the vaginal microbiome 
Sake tasting has successfully redefined my impression of sake (from warm spit to yum) Fave was nigori style unfiltered silky mild
Great session on food safety hosted by PSAB. ~400 attendees.
 Check out student posters 523 & 524; 15 & 16 year olds doing HIV research! Tweet me to chat with them!
 Hope the sake sampling has a positive effect on later sessions and posters.
Now  has shown up at the sake tasting. It's a science party. No seconds on koji mold-infected rice, though. 
The marine biologist in me found the alcohol at the microbio mtg. The  crew would be proud!!
 Congrats to Nhi Khuong from the Rothman-Denes lab for Nestlé Div M award...talking about N4-encoded inhibitor of cell division.
: All scientific conference sessions should conclude with sake tastings. ” AGREED
Yay its great so many people are tweeting ": still trending on Twitter. ^gm"
 Feiss: how did differences in structures/complexity of cos/terminase complexes evolve?
Sake tasting happening now in Room 110 at  - love this meeting!!! 
The more polish on the rice, the less protein left before fermentation. The lighter more floral the taste. Ginjo type 
Audience comment: HMP studies in indigenous communities practice scientific colonialism if they don't include local collaborators
All scientific conference sessions should conclude with sake tastings. 
Ethics of HMP studies: overstepping informed consent can result in consequences - like return of samples & huge fines 
Diversity & ethics of the human microbiome project: partnerships w/ community should have usable outcomes , not just possibilities
Wait extracellular atp increases extracellular dna and enhances biofilm formation? 
Biofilms are critical for infection of rice xylem. Ax21 (quorum sensing) knock-outs of Xanthomonas have reduced virulence in rice.
 Div M meeting. Mike Feiss will talk about DNA packaging
Ax21 is quorum sensing molecule produced by Xanthomonas. It's recognized by XA21 (toll-like) protein in rice. 
Exhausted! Busy day at poster 495 baby poo time series metagenomics. Thanks to all who visited 
MT  Wild progenitor of carrots had white or purple roots. A couple of genetic modifications led to orange carrot of today
 suggestions for next year: include a pad of paper and a pen in the bags please
 medical Mycology seems to be poorly represented in today's posters :( too many bacteriologists!! :D
Xanthomonas oryzae causes bacterial blight in rice. 
XA21 surface molecule in rice recognizes conserved microbial signatures 
 interesting poster frm AZ St U: autistic children hv less diverse & imbalanced gut microbiota, esp those that break sugars
Move over golden rice, scientists may move to putting carotene synthesis pathway into residents of the human gut microbiota
. Future approaches to alleviate VAD. engineer microbes in our intestinal tracts to produce vitamin A
 Found maize gene was much better than daffodil. Got 40% increase in carotenoids. 1 serving golden rice delivers entire RDA 4 child
. Syngenta took up the project to try to improve levels of b-carotene. tested a bunch of psy genes.
. golden rice version 1, 2000. Science paper showed can produce carotenoids in rice, but not enough
. In 1999, Davis Calgene group expressed Erwinia gene in canola. Found massive increase in carotenoids, mostly b- and a-carotenes
I don't get star struck by movie stars but I do get fan girl around science stars!!   
. Golden rice project started in 1997 with funding from the Rockefeller foundation. Put daffodil gene into rice to mske phytoene
Liveblogged ASM Live livestream When Good Bugs Go Bad: Microbiome Dynamics and Disease  on Google+:...
 in plants, carotenoid pathway is similar to E coli but plants differ in middle of pathway.
 Carotenoid gene cluster from Erwinia isolated and functions of genes determined. Set stage for development of golden rice
Penises, vaginas and bowels (and their microbiomes) done, now for lunch with 
. Microbial geneticists at Chevron isolated yellow E. coli and Erwinia herbicola variants.
Two recessive mutations led to orange carrots: first mutation created yellow carrots, 2nd made orange. 
Microbiome: CR: not enough info to connect race+microbiota+IBD at present, but links b/w race/ethnicity+microbiota provide clues
American Society for Microbiology meeting: A lovely weekend spent with gut bugs. 
CM Mentoring session, round 2; stop by the  CM Lounge (rm 112) at 1pm for Dr. Carey and Michele Pessa! ^tg
. The genetic diversity of rice does not permit a classical breeding approach. There are no known yellow genetic variants
Wild progenitor of domestic carrot was white! People like colored food, chose to breed the mutants. 
 Wild progenitor of carrots had white or purple roots. A couple of genetic modifications led to orange carrot that we eat today
 Or can alleviate VAD by eating mash of immature green rice or take vitamin supplements but is costly and often doesn't reach poor
: Poster presentation on new algae resource going down very well at  general meeting, lots of interest”
 VAD can be alleviated through dietary diversification. Eat more yellow, orange and green foods, eggs and dairy.
Talked to scientists from all over the world today interested in what we have going on in little Utica  
 Conversion (cleavage) of b- carotene to retinal is enhanced in presence of oil
Carotenoids have high conversion to retinol when oils are present; much less efficient in absence of oil. 
. Milk, margarine, carrots and eggs are the major sources of vitamin A in US diets.
  it's a beautiful world now that we have our choice of hot or chilled Sake. Wish I had opted for your session not eColi.
Microbiome: Courtney Robinson discussing race, microbiota, and IBD 
. Green plants provide ca. 1/3 of vitamin a in US diets (except vegans get 100% from plants)
Vitamin A deficiency is most severe Africa and SE Asia, affecting children and pregnant women. Leads to blindness and death.
 as many as half million become blind every year and 1/2 will die
 vitamin A deficiency is a severe health problem in 1/2 the world, especially in Asia and SE Asia
 paul karrer and co-workers in 1930 determined the structure of b-carotene and vitamin A. Awarded Nobel prize.
At American Society for Microbiology meeting, we've heard about vaginas, teen penises, and inflamed bowels in 1 hr. Good morning!
. Vitamin A discovered in 1913. Present in butterfat and egg yolk, essential for growth and development of rats
Poster presentation on new  algae resource going down very well at  general meeting, lots of interest
Are you at ? Tell us your favorite aspect of the conference! (Elsevier booth is #1213 - stop by and say hello!)
. Francis Cunningham will now speak about golden rice
sake, miso, tempeh, oh my! RT : . and I are getting extremely thirsty listening to this Sake talk. Anyone else?
Come see us at  in San Francisco today! We're at booth 1518
Matai: Junmai sake is sort of like Japan's version of German beer (only rice & water). Ginjo sake invented less than 20 years ago.
. and I are getting extremely thirsty listening to this Sake talk. Anyone else? 
. Today there are 2 basic types of sake. Junmai, old world sake that you drink warm and Ginjo, new world sake- drink chilled
Izumi Matai showing more 16th century woodcuts as method illustrations. Awesome. 
Tweets from concurrent  session ('culture of rice') causing major lunch cravings
Pasteurization of sake began over 300 years before Pasteur
The penis, a different story: Different ethnic groups in US have very similar microbe colonies, sez Indiana U's Fortenberry .
It seems all the  tweeters are in this history of sake talk right now. High twitter usage correlates with interest in alcohol...
: Attending ? RT this and stop by booth 1617 for special giveaway” I love surprises
Sake mash relies on nitric acid reducing microbes and lactobacillus to repress wild yeasts. 
Sake started out as a drink for the nobility. Isolation/purification of Koji mold allowed mass production & wide popularity 
 Mass production of sake and other fermented products began in the 13th century, including miso and tempeh
 culture of rice talks are great. Lots of rice history $ fabulous photos. My talk will be very modern, will only reach back to 1845.
  Enjoying your tweets from  Do you guys already know each other? If not, try to find time for a drink together
Koji mold (Aspergillus Oryzae) is the key ingredient in the sake fermentation process. no delicious alcohol without it! 
Microbiome: penis microbiome: DF studying behaviorally mediated interactions among microbiota over time in adolescents 
Huh. Didn't realize that sake is fermented by Aspergillus (koji) isolated with alkali ash. 
Microbiological serendipity -- how did the Japanese discover the safe koji mold when it's "evil twins" produce deadly aflatoxins?
 Ash used to keep Koji away from contamination. This advanced technique sometimes still used today
Attending ? RT this and stop by booth 1617 for special giveaway
Mass production of pure koji mold in Japan in the 13th century!
 In the next few hundred years, many types of sake made. Nobility drank filtered sweet sake.
. Airborne mold infected rice in the 8th century. Thus launched sake brewing.
Fortenberry: clothing, hygeine, circumcision, condoms, sex practices are ways culture could affect penis microbiome.
8th century koji mold discovered, early sake brewing begins, sake becomes drink rather than eaten product 
Izumi Matai showing 17th century woodcuts; wish more talk would do this! 
"the moldy rice developed into a delicious beverage called Sake"
When good bugs go bad - ASM Live at noon PDT, join us online, ask questions on twitter at 
 Do some enteric bacterial pathogens have a two-host life cycle? Session 043 Dr. Fletcher
Microbiota of the teenage penis by d fortenberry Begins w a primer on postcolonial literary criticism  
Original sake 2000 years ago mash was done by chewing
I'm here! RT  Next speaker in "culture of rice" session is Izumi Motai from the Takari sake brewery in berkeley
To make sake: 1. Make koji (grow mold on rice) 2. Make mash (fungal enzymes break down starch add yeast) 3. Ferment
10 mins in to this talk and yet to even hint at some experiments/results  
Photo: Moscone Center, San Francisco - site of  (Taken with Instagram) 
Now onto the guys! Dennis Fortenberry: is race meaningful for penis? Biological or socioeconomic differences? 
Microbiome: w/i race/ethnic group differences may be just as large or larger than b/w group differences J. Dennis Fortenberry
I give my Roche-provided latte a rating of 4.54 out of 5 . I paired it with some syrup. 
Forney; Human Microbiome Projest excludes women w/ high vaginal pH. That is most women. 
Roche have an espresso machine. Just ordered a latte. 
The most important message from  : lactic acid bacteria -> beer spoilage, AAAAAAAA!
MT  Eva Harris: brilliant surveillance of dengue & flu in Nicaragua conducted despite unique challenges cc
Cool, vaginal microbiome shows we need to stop labeling women as "normal" or "not"- redefine healthy to reflect diversity! 
Forney: stop making averages abt women, 40% of black&Hispanic women have unhealthy vag. microbiome according to"conventional wisdom"
Healthy black, hispanic women have diff. vaginal bacteria than white, get labeled abnormal, sez microbiologist Larry Forney.
 if anyone sees Bill Margolin, send him to poster 300
Rice plus enzymes (mostly amylases) from koji mold (Aspergillus oryzae) turns rice starch into sugar for fermentation. 
Microbiome: vaginal microbiome: "need to think critically and re-evaluate 'healthy' and 'normal'" (LF) 
Being around this many microbiologist makes me realize why I love science 
Larry Forney: vaginal  varies btwn ethnic groups, normal in black & Hispanic women cld be misdiagnosed as disease
Just talked to PacBio people at . Very friendly. Seems like very long reads will be great for de novo assembly with Illumina.
Japanese scientist Jokichi Takamine one of the fathers of biotech--filed a patent on a microbial enzyme in the 1800s 
Microbiome: vaginal microbiome composition: prevalence of community-state types vary among ethnic groups Larry Forney
Larry forney challenging the use of 'normal' to describe vaginal ph and microbiota. Science w conscientiousness 
The  exhibit is hopping! Come by booth 310, take a short survey on next gen seq and get a $10  card.
Forney: the idea that vaginal health is indicated by ph <4.5 simply isn't true especially in hispanic and black women. 
Fermentation to make sake is more like making beer than wine.
Dr. Steven Specter, notes ASM int'l programs bring the goods: Good people, good trainers, good facilities, good resources. 
Forney: speaking of function as opposed to taxonomic name, lactic acid bacterium rather than lactobacillus will reduce confusion.
Enjoying my trip to SF - hanging out with microbiologists of all levels in the Clinical Microbiology Lounge at !
Disease triangle: host, pathogen, environment 
Forney: we need to rethink what we thought we knew re: vaginal bacterial ecosystems. They are not all the same and are not stable.
Tanbo art uses rice plants with differently colored leaves to make intricate murals in rice fields 
Learning more about ASM's Laboratory Capacity Building activities under PEPFAR.  
Guess I'm in San Fran! Thx 2 all my  tweeps 4 a great time. Ur energy feeds me. Luv my job! On to 
At  tweeting for . Racial/ethnic differences in  session is packed
In my Hilton (!) hotel room. Staff was wonderful. Time for a Microbe Library Board Meeting.  
Cool RT : Watch online and tweet in questions for ASM Live microbes in buildings and hotel rooms
Kim Orth just showed that a T3SS associated with clinical Vibrio para/cholera isolates regulates an intercellular infection 
Sepsis Know from Day1: Microbiology Approach. Join Grady Health System speaker bioMérieux InBooth Knowledge Forum 12.45pm booth1301 
Wow, so many people here. Like a Salmon swimming upstream trying to get to microbiome racial/ethnic differences session
Stephanauskas: Could every microbial cell be genetically different? Especially "uncultured majority" in oceans 
ASM Live at 11AM at  - bacteria in hotel rooms and microbes in man made environments watch online and ask questions on twitter
 It's all the clinical folks. You can pick out microbial ecologists pretty easily 
ASM Live webcast: The most contaminated surfaces in hotel rooms. Starts in 10 min   ^js
ASM Live webcast: The most contaminated surfaces in hotel rooms. Starts in 10 min   ^js
Perfect: the ability to test and treat infections early at bedside (about $36) can have major implications for saving lives.  
Just informed by speaker that a sake tasting will follow history of rice session! 
Yes! It's fun to hear what's up elsewhere! RT : Good to see a critical mass of livetweeters at ! Keep up the good work!
Giovannoni: fan of metagenomes, but need our little friends (cultured microbes) to help figure it all out 
Whoops. I just applauded Steve in the overflow room. I was the only one. Guess I got caught up. 
 when you have genomes, time series data, and cultures, you can do some cool stuff! 
Hey  ppl! Come meet us at booth 1625. We'll explain all about   tool & give cool gifts. See U there.
The exhibit hall is open at  and we are looking forward to meeting you! We're with the Bugbox at booth 1534.
 This seems to be a very powerful system for understanding the genetic basis of ecological variation! 
SG: getting in to our recent project on the high core genome conservation of multiple SAR11 strains 
Check out the Careers in Microbiology symposium at 11:00, Esplanade Ballroom rm. 304!   ^lv
Cultures may not always be informative about other closely related organisms thanks to rapid diversification - SG 
Good to see a critical mass of livetweeters at ! Keep up the good work!
Giovannoni: need extreme patience to culture microbes that divide once every 5 days. 
Microbial communities: single-cell  could extend the reach of HTCL to vast parts of uncultured microbial diversity (SG)
SG: Some questions you just can't tackle without access to cultures (ex. of inducing gene expression) 
SG: figuring out the role of proteorhodopsin in SAR11 required cultures 
Giovannoni : All you oceanographers know that didn't make any sense. 
 John Perfect dramatizing the diagnosis of IRIS in a tx pt w great vigor & Shakespearean flavor; refers to damage-response frmwrk
Mystery effector vopQ induces rapid autophagy in v.para, w/in 5mins when expressed recombinately. No known structural homolog!
SG: Will expanded insights from single cell genomics change our view of microbial genome evolution? 
Giovannoni  'Change perspectives of microbial cultivation, I run my own culture collection, this has a big scientific impact'
Did anyone catch which city's subway map Giovannoni just had up on the screen as he was talking about systems biology? 
Microbial communities:  reduction comes at a price: unusual, tricky nutrient requirements = difficult to grow (SG)
Infection nucleoid=rosette of phage assembling at cell center, if not localized reduced burst numbers 
Many marine microbes are difficult to culture because they have unusual metabolic requirements - SG at 
Giovannoni RT  SG jokes "Don't start a culture collection!" at 
SG: the result of genome streamlining is unusual nutrient requirements, which makes these organisms hard to grow.
Anyone else in San Francisco pick up a little food poisoning at? Need to compare notes.
Giovannoni: Vibrios & Alteromonas can reach high abundances episodically but otherwise undetectable most of the time. 
Is there evidence for strong selection of genome streamlining in marine bacteria as per Giovannoni? Deletion bias possible?
SG: genome streamlining comes from "a very powerful selective hammer on these cells" 
RT : SG: Streamlined genomes are small because of selection, symbiotic genomes are small because of drift. 
SG: Streamlined genomes are small because of selection, symbiotic genomes are small because of drift. 
SG: symbionts get reduced genomes thru a very different evolutionary process than streamlined genomes of marine bactioplankton 
 J. Pogliano. PhuZ localizes phage DNA replication and packaging to midcell. "infection nucleoid"
Phage with their own genes for tubulin-like proteins (PhuZ), need the proteins to localize their DNA to center of cell 
Giovannoni: not funded to run a culture collection, but does it anyway ~ 2500 isolates; culuring matters 
SG: discussing his experiences with running a culture collection- lots of people use our cultures now! 
Very good talk by Sir Roy Anderson on data and databases available for infectious disease. 
SG jokes "Don't start a culture collection!" at 
SG: but to get the dominant organisms, like SAR11, you need cultivation with dilution to extinction, other specific techniques.
SG: I'm not funded to run a culture collection, but I'm running one right now! Housing about 2500 strains right now. 
Microbial communities: consider life cycle history of 'unculturable' microbes Stephen Giovannoni 
Struggling to hear a little in single cell session - note to self: don't rely on microphone to do all the work! 
Dilute cells and develop sensitive detection protocols: the key to culturing marine bacteria 
SG: Disturbance-loving bacteria can reach high abundances during episodic blooms, but otherwise undetectable most of the time.
SG: those guys are adapted spurts under optimal conditions.
Global warming contributes to persistence of vibrio parahaemolyticus, causative agent of oyster related food poisoning
SG: what are classical, easy to culture organisms normally doing in the water? They're really rare. 
: Pearson: Good news for using lipid biomarkers in the fossil rec ” Our TP uses "I cant believe its not lipid biomarkers".
SG: r vs k, generalist vs specialist are old concepts that need updating. We're missing life cycle history 
SG: Pervasive concepts in culturing microbes: metabolism, substrate, growth rates, nutrient concen. No one considers life cycle! 
Life cycle complexity is more rule than exception: Giovannoni
Christian Munz: lots unpublished data so will be vague. Macroautophagy: special subset, LC3+ phagosome that promotes APC activity. 
SG: documenting the attack on culturing the "uncultureable"
 Eva Harris gave a fascinating look at some brilliant surveillance of dengue & flu in Nicaragua conducted despite unique challenges
Simulating spread of influenza A on global map via Microsoft. Very quick spread. Chilling. 
Steve: when the great age of economic excess is over, we're all gonna be interested in geochemistry 
My AT&T 4G connection is so much better than the Moscone wifi :( will my data plan run out?
Stephen Giovannoni: changing perspectives on microbial cultivation; undetected to unculturable to uncultured 
Last talk of the morning micro community session: Stephen Giovannoni talking about expanding the cultured microbial world
Steve has received the Porter award this year 
Now my boss, Steve Giovannoni! The Porter Award winner for bridging the gap between culturing and culture independent techniques 
Steve Giovannoni - Expanding the Boundaries of the Cultured Microbial World (via 
However, - only 1 abstract on Giardia and 3 on Entamoeba histolytica! 
Pearson: Measuring isotopic ratios of protein products will help us move towards using stable isotopes in metagenomic studies
One single abstract on Blastocystis at  - not impressed!
one of my favorite activities at ASM is to take a peek into the work going on in so many areas outside my every day world. 
Good use for spray sanitizer swag!RT : The most contaminated surfaces in hotel rooms   ^js
RT  J Pogliano: some phage make their own ftsZ homologs.
Negative feedback is one way to decrease the noise in a system. N. Wingreen  
 J Schaenman presenting fascinating cases explaining extent of invasive mycoses in tx pts -so far aspergillus cocci crypto gattii
 J Pogliano: some phage make their own ftsZ homologs.
Don't you date cough in a room full of epidemiologists!! 😖😷
Satellites scanning Earth using infra-red to identify population density for countries without census. 
 Joe Pogliano up now on how phage exploit host shape. Audio a bit dicey...
Pearson: Hopanoid biomarkers not specific to cyanobacteria as previously thought. Mostly proteobacteria. 
iPhone app to map sequence data to other strains based on MLST. Google maps interface with relationships geographically shown.
Roy Anderson presenting some interesting data & information using social media to track emerging infectious disease trends. 
Possibilities to predict disease outbreak using twitter and Facebook.
Using social media to predict and track disease outbreaks. Facebook and twitter!!
 genome gazing depends on what's in the databases. Lean how to help in 078 4:45 today. CACAO:Competitive Annotation of Gene Function
Pearson: Bacteria mostly make hopanoids & not steroids--good news for using these compounds as lipid biomarkers in the fossil rec
MT : Simulating influenza A movement in UK based on mobile phone data ” -Google did this, too, no? Based on searches
Following changes in movement after cholera out break in Haiti using mobile phone data. 
Simulating influenza A movement in UK based on mobile phone data. Use simulations to make policy. 
MALDI-TOF: Positive impact on results & workflow. Join C.Ginocchio bioMérieux's In-Booth Knowledge Forum at 11.50 am, booth 1301
For any microbio groupies wanting to follow along at home RT: Watch ASM Live now at 
Using mobile phone data for modeling epidemiological movement in communities. Very nice idea. Excellent visualization too. 
 John Perfect sitting there on the podium looking intense & leonine as ever: Up next on immune reconstitution inflammatory syndrome
 my bad. Joanna Schaemann of UCLA next, on IFI in Solid Organ Transplant pts; almost 30K tx/yr; significant morbidity-mortality
 one zettabyte = 1 trillion gigabytes and the world already contains more data than we are capable of storing. I need a 3Z phone!
Poster hall at  is massive ! Epic number of posters being presented
Microbial communities: targeted manipulations to enrich for organisms with certain characteristics in  studies
Pearson: looking for biomarkers that can be used to trace microbial groups back in time in geologic record 
 K Young: Lpp needed to recover but not maintain rod shape.
 Some of the talks are being featured in ASM Live, our online talkshow starting at 10 today.  ^js
Ned Wingreen "bacteria are multilingual" - universal signals as well as species-specific ones 
Pearson: lipids from oceanic archeal species suggest they are chemoautotrophs living below the photic zone. 
Pearson: Can investigate functional roles of microbes by measuring how heavy cellular lipids are. 
Geoffrey Smith: virions extracellular, host nucleated actin=cytoplasmic. virus tags and can distinguish inf and uninf cells. Sweet! 
Ned Wingreen giving a simple intro to quorum sensing - old hat for many but great for those rel new to microbiol like me! 
To  attendees - I'm living vicariously through you. KEEP ON TWEETIN'.
AP: Thaumarcheal lipids are isotopically heavier than phytoplankton, fractionation helped them track down the c-fixation pathway
 Are any of the talks from the  being webcast? if so where, how to listen?
Geoffrey Smith: great talk. Video microscopy reveals vaccinia virus "hopping" from inf. to uninf. host cells on host actin tails 
Geoffrey Smith jokes that maybe medical students today should get attendance certificates like those given out by Lister 
Jennifer Philips: neat mechanism proposed. EsxG-EsxH binds and inhibits ESCRT complex that drives maturation. 
Jennifer Philips: RNAi screens in fly and mouse uncover TB protein complex (EsxG-H) that blocks phagosome maturation 
The avoidance and subversion of host cell defenses by intracellular pathogens session has been amazing so far! 
Geoffrey Smith shows certificate of attendance signed by Lister for 11/11 lectures in 1871 by his Great Grandfather 
Def need to learn more about nanoSIMS after this morning's talks
Accurate assessments of cell viability, apoptosis & cell cycle in minutes w/   
 Kevin Young showing interesting shapes as E. coli recovers from spheroplasting
Had no idea that diagnostic microbial lipids could be preserved in the fossil record.  So cool! 
AP: reverse TCA fractionates carbon less than Rubisco, so this can be used diagnostically for different c-fixation events 
At 10:15 am PT Stephen J. Giovannoni, who has won an award, will be giving his lecture in the Esplanade Ballroom 305/307. ^lg
Patches of inert peptidoglycan located at tips of E. coli branches
William Jackson: acidification of lysosomes required for very last step in poliiovirus maturation. ...and they found it last :-) 
Microbial communities: who is doing what *with whom*? Jonathan Zehr 
Using lysozyme to make E. coli spheroplasts -- another example of doing something "everyone knows can't be done" 
Ann Pearson: using lipid biomarkers and stable isotopes to look at biosynthetic pathways in microbes 
me neither MT “: Pearson said "you probably see your fav species". No eukaryotes, so I don't see mine :( 
 lactobacillus 1M CFU can reduce candida infection in neonates? Hmm.  one good effect of 'probiotics'? ER speaking.
AP: how does functional diversity of microorganisms get reflected in the geologic record? 
Pearson put up photo collage of microbes and said "you probably see your fav species". No eukaryotes, so I don't see mine :(
Vic Orphan nanoSIMS on aggregate slices to dramatically increase resolution - individual cells! Very slick. 
Listening to cool talks on bacterial morphogenesis at 
Awesome! RT  Look at that,  is trending on twitter. Microbiologists, unite!
RT  Ann Pearson--Exploring natural microbial communities w/ lipids and stable isotope ratios. (via
Ann Pearson from Harvard on lipids and stable isotopes 
Ann Pearson - Exploring natural microbial communities using lipids and stable isotope ratios. (via 
Almost all microbial community talks this morn have mentiioned NanoSIMS. We need more use of this awesome imaging technology!
Inert peptidoglycan patches not recycled or diluted by new material
PBP mutants have abnormal Z-rings, cell constriction altered, leads to freaky shapes in E. coli 
 beta glucan detection - works so well for adults; problematic in children. Uninflected immunocompetent children show high values
Look at that,  is trending on twitter. Microbiologists, unite!
Super sore today, but it will be fun to exhibit at  Come visit me in booth 1306!
Note the typo in that last post, it's Zehr. My fingers need more caffine 
Nano SIMS let's you see an image of where different isotopes are located within a single cell, that is super cool. 
Zehr: making a marine functional array for major microbial communities in the oceans to examine interactions. Wow. 
YES RT  Anyone else finding the seats at excruciatingly uncomfortable? 
JZ: nitogenase distribution data showing everything is NOT everywhere. 
Zehr: Who is doing what...and with whom? Symbiosis BTW cyanobacteria & calcifying eukaryote. 
Learn more about career opportunities for CMs today at 11am and 1pm in the  CM Lounge (rm 112)! ^tg
Just watched footage of a virus particle being moved around by actin tails. Cooooooool 
Heard John Taylor couple of weeksago use the phrase "reverse ecology" for genome directed understanding of ecology changes
Zehr: Genome sequence of cyanobacterium revealed many missing enzymes for metabolism, sugg probably asymbiont 
Zehr: For microbial communities, it isn't 'who's doing what', but 'who's doing what with whom'? 
JZ: improved handling revealed UCYN-A was a symbiont with a haptophyte 
 Victoria Orphan in Single Cell point of view session: integrate microscopy, -omics, nanoSIMS, isotopes. Anaerobic CH4 oxidation
Getting my science conference on. So far so awesome 
Sensing a trend here at  , genome gazing leads to otherwise unpredictable insights into bacterial lifestyles and physiology
Loving the marine biology at  - Jonathan Zehr now talking about Cyanobacteria and picoplankton associations & genomics
 neonates at highest risk for candidiasis, low birth wt, premature birth, all risks. candida 3rd frequent cause of neonatal sepsis
Zehr: symbiosis suggested from lack of metabolic pathways in genome seq. of cy anobacterium 
JZ: traditional procedures were giving them erroneous sense of what n2 fixers were free living. 
Patches of actin-like proteins move in circles along longitudinal lines during cell wall formation in B. subtilis--not fixed helix 
Really enjoyed the live tweets from ! Now I look forward to following !
 Emmanuel Roilides from Aristotle U in Greece up next: on invasive candidiasis in NICU (neonatal ICU); neonates highest incidence :(
Lidstrom - Both culture independent AND culture-dependent approaches important to work out key environmental processes
 You can't have a fancy-Sci-fi-worthy stage AND nice seats! It's California but still, not Hollywood! 
Zehr: endosymbiotic Cyanobacteria are missing genes for some key cellular machinery 
JZ: nanoSIMS used to prove n2 fixation by symbionts and uptake by associated cells 
JZ: N2 fixers many times in symbiotic relationships 
 Well, I suppose it could be good in a way. It was a popular talk! 
JZ: Trichodesmium a charismatic microorganism! 
Slow wifi & broken phone impeding tweeting! Time to get the laptop out to report on 
Hey, who let the  people in? Poster 584: BioCurious - Moving Biotech Education Beyond the Classroom, 10:45-12:30 
CDC's John Nkengasong highlights  EID Fellowship Program, role of lab surveillance, at 
Zehr discusses balancing the Global Nitrogen Budget at (eat your heart out congress!)
Anyone else finding the seats at  excruciatingly uncomfortable? 
Sounds like global meeting for American Society of Microbiology is going on now at 
Want to meet the people behind !? We are exhibiting at  come say hi at booth 1306 and you may win a prize!
John Zehr from UCSC talking about nitrogen fixation. 
Can't use laptop-slow WiFi + scorching my lap. I can't walk & chew gum (listen & type w 1 finger on phone). Glad  is on it.
Really, thats terrible! MT : Huh. Official ASM people are throwing 20 or 30 people out of my session. Not enough space.
Join us for ASMLive at  at 10 am for insights into conversations in your nose and microbial neurotransmitters in your gut
Reminder: we have a public friendfeed group going for notes. Feel free to add to the archive:
Cool pics of B. subtilis morphological mutants (cytoskeletal mutants) -- one looks like a corkscrew 
I've made it to  after a little BART delay. Watched Jill Banfield's talk from the floor, but now I'm plugged in and ready to report!
RT  “ wifi is free but sloooooooooow.” too many simultaneous connections :(
Shapiro: Caulobacter chromosome segregation is highly organized & dynamic, starting at cell pole 
Stochastic noise in flagellal activation results in exploratory movement. Very nicely modeled using exponential distribution.
 Microbial Communities session. Room is full, we can only hear who presents. And we're not allowed to sit on the floor.
Hell of a lot of genomic data in Jill Banfield's talk...way to dense to process right now 
Huh. Official ASM people are throwing 20 or 30 people out of my session. Not enough space in the overflow room, even. 
All Jill Banfield's trees are for ribosomal protein S3...don't know whether that's good or bad 
 disseminated candidiasis by Candida tropicalis, deep tissue infection, difficulty &gt; albicans - Tom Walsh; problem in India too
New Revco UFX400  freezer usesup to 15% lesson energy.  Chk it out  booth 1015
 all cases of candidemia require treatment; morbidity, mortality similar to bacteremia by staph aureus - Tom Walsh
Pseudomas use of phenazine as e-acceptor in CF patients great way to show relevance of metabolism to students 
Statistics of single-cell single-flagella. Very nice modeling. I like mathematics in biology talks. 
JL: most organisms that came up during acetate stimulation were not the same as the background organisms. 
I see self organizing maps on the screen; I must have found Jill Banfield's talk. :-) 
 Hurray for ultramicrobacteria! Sometimes strategy for starvation? 
Caulobacter talk by Lucy Shapiro nice blend of system/reductionist, echoes of opening session 
We are live from ! Stay tuned for blog updates and tweets from the sessions today thru Tues. And stop by booth 310 to say hello.
JL: most abundant organism at Rifle is RBG-1 candidate division, never before observed. 
Toss out those 0.2um filters environmental micro tweeps! Banfield tells us we are missing the little bugs 0.1um is better 
Congrats to Geoffrey Smith, former SGM  Council member, for award of ASM International Member 2012. 
Now cryo TEM data from Birgit Luef showing very small cells
Just turned off autocorrect -- scientific freedom! 
JL: OP11, OD1 - hypothesis: the cells are small 
JL: many of the assembled metagenomes went to candidate divisions, and these are organisms with <1% abundance 
Kelly did an insane amount of genome binning, assisted with time series abundance info. 87 genomes! 
 Tom Walsh on New challenges posed by Invasive Mycoses in pts w Hematological Malignancies: now talking abt existing antifungals
Banfield: bin meta genomic data by tetra nucleotide freq to assign to organism 
The exhibit hall is now open at . Stop by to see us at booth 532 to learn about   tests and resources.
JL's talk centered around data created by Kelly Wrighton 
Cellular machines = "somes": divisomes, inflammasomes....
JL: acetate amended sediment communities at Rifle bioremediation site. Then metagenomics and proteomics. 
 Golding discussed the decision between lysis and lysogeny studied at the single cell level.
: microbiologists are much classier than marine biologists by the looks of  - think I'm moving into the right field.” fight!
JL: things have changed with metagenomics - going after complex communities now 
I'm tweeting from here today! RT  those interested in microbes, follow the action at the ASM meetings via 
Geoffrey Smith, award laureate, will be speaking this morning at 9:15 am PT at the Convention Center in room 104.  ^lg
Again for those interested in microbes you can follow the action at the American Society for Microbiology meetingw via 
Next- Jill Banfield from UC Berkeley. Go bears! 
ML: Need to couple culture dependent/independent approaches to identify key players in microbial communities 
ML: 9 single-cell genomes in the pipeline now, 2 from Methylobacter. Microfluidic sorting still amazes me! 
: It wd be kind of nice if the scientific program actually said WHERE the talks are taking place ” get the app; v helpful
Poster loaded & ready to go at 10:45!  the poster hall is huge; over a thou posters on just this first day!
It would be kind of nice if the scientific program actually said WHERE the talks are taking place. Grumpy. 
I love this combination if culturing and culture independent techniques. We do that too, of course.  
RT : ML: metatranscriptomics helped them figure out how to culture methylobacter. Key was to limit Methane 
ML: metatranscriptomics helped them figure out how to culture methylobacter. Key was to limit Methane 
ML: Methylobacter needed low methane and low O2 
ML: Methylotenera possibly using oxidation of C1 cmpds for energy and other carbon for biomass. 
 Luis Ostrofsky-Zeichner detailing Risk Factors & Diagnostic Markers for Candidiasis in ICU Pts, emphasizing need for better markers
Dear : Seriously? No trains until after 8:15 today? No wonder Californians have to drive everywhere.  
  starting to enjoy conference feeds keep it up - maybe go blogging for reference selections ask maybe?
 You forgot metametabolomics. All these paralell approaches are amazing! 
Bryan Schmitt, D.O., discusses Identification of Corynebacterium species, among other topics, today at 10:45am at .
They're throwing the whole book at this: metagenomics, genomics, SIP, transcriptomics, culturing, single-cell analysis 
 metametametabolomics is looking at metabolites of all your communities at once 
 wifi is free but sloooooooooow.” too many simultaneous connections :(
ML: change of concept methanotrophs sustain larger communities, starting to look at the whole community as a "metaorganism"
ML: sequencing 80 new genomes from the isolates. 
Mary Lidstrom - sequencing 80 new genome sequences from methylotrophs cultured from Lake Washington 
ML: combination of pure culture studies and metagenomics. Over 100 new strains that are relevant based on metagenomics.
 fortified w Starbucks coffee, egg-spinach-cheese crepe, onward to symposium: Mngmnt of Invasive Fungal Infections in HighRisk Grps
Mary Lidstrom is up first- C1 cycling in the environment. 
First up this morning- Who's Doing What in Microbial Communities.
Ok, session about to start. Radio silence for a couple of hours I'm afraid  
Jim Collins: lots of interest from industry for engineered kill switches to counter corporate espionage  
microbiologists are much classier than Marine biologists by the looks of  - think I'm moving into the right field.
Jim Collins told to stick to engineering as biology is too complex and later that that were so naive that they might just succeed 
New Insights in Global Surveillance of Current and Emerging Infectious Diseases.  (@ Moscone Center)
Dianne Newman: bacteria use antibiotics as electron acceptors/donors in the environment 
Great, funny orientation talk from Wade Bell - take opportunity to expand ur horizons while at 
The Moscone parking garage opens at 9am but the sessions start @ 8:15 am. WHY 
Scott O'Neill: local release trials show high Wolbachia rates in mosquito infection up to 2 seasons after releases stopped 
Scott O'Neill: community engagement for Wolbachia mosquito release used telemarketing approach, still got big positive response
In session "Who's Doing What in Microbial Communities" - words on screen seem a bit blurry no matter where I sit; focus, please?
Pop by booth 936 for a chat and pick up a mouse pad and a demo CD of the latest release of MacVector. Enjoy yourself at 
Some points from last night opening session at  to follow...
Morning session at  on avoidance and subversion of host defences. Will post some key points later tonight.
Come meet our guys at booth 1625  today. They're really nice & would love to show U what 's all about+give U our cool swag
Fantastic time at  in San Mateo, really great conference, but now time to relocate to downtown San Francisco for !
Have planted myself in the "whose doing what in microbial communities" session for this morning. Banfield, Lidstrom, Giovannini 
Come hear the first award laureate lecture of ! Susan Sharp is giving her talk today at 8:15 am PT in the Esplanade Ballroom 304.^lg
What a difference from my last meeting, where I was the only tweeter!  
MT : Getting ready for  today, come visit our booth 1625 & learn about . If you see this tweet & attending let me know.
MT : Getting ready for  today, come visit our booth 1625 & learn about . If you see this tweet & attending let me know.
Good morning  ppl! Come meet us at booth 1625. We'll explain all about   tool. Have a wonderful day :)
Good morning  ppl! Come meet us at booth 1625. We'll explain all about   tool. Have a wonderful day :)
At ONT waiting to board flight to OAK. Will I get to my grad students poster? Also, Happy Father's Day to the travelin' microdads
Accurate assessments of cell viability, apoptosis & cell cycle in minutes w/   
Getting ready for  today, come visit our booth and learn about  . If you see this tweet and attending let me know.
Awakened instantly from a deep sleep by bird singing, "Shut up! Shut up!" outside my window. I love parrots! 
Kind of inside baseball, but I have long wanted to give an ASM GM talk. So here I am; fingers crossed! 
 not to worry I am coffeed up, on the bus saying hi to my tweeps and on to my 1st session with time to spare. 
 , booth 1415, publishing editors and will be there. We love feedback & tips. Hope to see you there!
Of course I am wide awake at 5:30 am in SFO. Dressed & ready. Thank God for my Starbucks app as I walk with a mission.
 MicrobiologyOpen meet-the-editor event today from 2-4pm, Wiley-Blackwell Booth 724. Free cookies and T-shirts! ...
 MicrobiologyOpen meet-the-editor event starting right now at the Wiley-Blackwell Booth 724 
 MicrobiologyOpen meet-the-editor event today from 2pm, Wiley-Blackwell Booth 724 




Just saw so many of my favorite microbiologists! Lisa Gorski, Julian Davies, Ned Ruby, Margaret McFall-Ngai, Eric Stabb... 
movie trailer voice over guy doing the announcements for opening session. Very stern for California!
Presenting a poster at ? Beat the crowds and pick up your networking cards now! Outside Hall C
Overheard today at  "I got norovirus. It was awesome!" ^js
About to walk down to  - making the most of sunshine :)
 microbiology career choices was a wonderful experience, a lot of good advices!
At ! Whoot. Seeking Tara and Jonathan, Mary, and Corrie...
Crowdsourcing for  - compiling a list of blogs that focus cover microbe-related topics - help needed
 heads to  General Meeting for the opening sessions... Watch out here we come!
I hope you have a great time!! RT : On the Bart to yippee!
 I'll be at  all day tomo a Doing a day reg for Sun - let me know if you might want a ride back tomo eve.
Be sure to stop by bioMérieux booth n°1301 at  for a personal tour through The Microbiology Pathway
Attention all  Poster Presenters! Don't forget to pickup your poster networking cards outside of Hall C. ^gm
If you have a poster at  Come by hall C now to pick up your networking cards  !
Opening of  should be good tonight -J. Collins on synthetic bio, S. O' Neill on Wolbachia/Dengue, D. Newman - on GeoMicro
 Here we come! Two postdocs giving talks, two student and a postdoc with posters. And I look forward to seeing everyone!
The amount of scientists on my flight and this train is ridic. 
Wow. I was not expecting it to be this HOT in SFO. I could have left the leather jacket home.  
Oh California how I have missed you. Getting ready for a great week in San Francisco. 
"Church is God forming a family out of strangers." D. Leong quoting Hauerwas
Registered for the  First a cup of coffee  and waiting for the opening ceremony. 
 it is a bad start when you get behind 3 women at hotel checkin and they begin their conversation with "we are going to a problem".
Wow. I was not expecting it to be this HOT in SFO. I could have left the leather jacket home. 
Dr. Icenhour is ready to mentor at the Microbiology Career Choices!
Oh well - say goodbye to some halophiles  restoration is good - but I will miss salterns 
Looking for a job in Microbiology? Attend the Microbiology Career Choices session at 1pm in Rm 103 to find out what's available.^gm
Last year I imagined going to  and tweeting like mad. Life got in the way. Depending on my microbe tweeps! Charge yer batteries!
 sitting in  waiting for my bird to Europe. Looking forward to your tweets from !
 CEO, Sean Bauman presenting a brief talk about global challenges of  tomorrow @ 2pm. Booth 1025.
Too bad "Stopping the Spread of Superbugs" isn't being presented at  or  Loved seeing it at 
Getting set to provide career advice at an early session for students interested in microbiology careers. 
Just landed at SFO now Hotel Monaco here I come. 
Thx! Miss u already! See u in San Fran! RT : Have to leave  early to go to . Best  ever! rocks!!
Register online for bioMérieux In-Booth Knowledge Forum at San Francisco  Presentations begin 2morrow 10.55am
And while you are on the  app, bookmark my talk at 4:30 on Mon 18th in the "New Bio Questions from Pan Genomes" section. Room 104
I don't know how I can attend conferences without own apps in the future. The  app is great for sitting in an airport n planning
Right next to you  RT : Getting ready to go to my first session at . Where my other microbiologists at?
Getting ready to go to my first session at . Where my other microbiologists at?
 I think they need to drop the 'express' from the pre-registration line... 
Hotel Monaco, breakfast at Talor Street Coffee Shop, and tonight
If you are in San Francisco early for  make sure to check out the microbiology at the Ferry Plaza Farmers Market. Yum!
Microbrew time is fast approaching at  , going to be good. Then off to  opening this afternoon! Busy day
Ran 3 miles in 27 mins and felt great. killer workout in SF before the start of  ! TM run with speed and hil... 
Stop by our booth 1301 tomorrow at  for a $5 Starbucks gift card & brief survey. Tell your friends too!
Registering for  doesn't include workshops. Separate, equal charge. Should've seen that. Teaching myself Pway-Tools for free, check.
Disappointed that I will miss ASM Communications Board meeting bc of time conflict w Branch Officers Forum. Next year! 
Sitting in on ASM publications board meeting this morning & Branch Officers Forum this afternoon.  
 Friend Feed is up. Posts will be the title of the talk, speaker name, and their institution. Talk notes... 
@ OKC airport on way to . Time to be geeky with the microbiologists
Small footprint & low noise is just 1 benefit of the Piko Real-Time PCR System  booth 1015
Nice! looks like  will be at  (for the weekend)! looking forward to  !
Important side note: There is a Body Shop & a Container Store walking distance from my hotel. Don't have them at home. 
Waters lab is at ! Looking forward to seeing friends and colleagues.
Time for an adventure at my first national conference! 
Off to ! Symposium tomorrow at 3: "Sound bites to Superbugs" on science communication with  and others, stop by!
Catch Dr. Robin Patel's presentation "Trends in Clinical Lab Identification of Microbes in 2012: MALDI ToF MS" today, 8:30am-4:30pm 
View Dr. Glenn Roberts' presentation on Lab Diagnosis of Fungal Infections: Identification of Filamentous Fungi today, 8am-5pm at.
MT  The A. Society for Microbiology and A. Society of Missiology  are both meeting now using .
Jealous of all of my friends and colleagues heading to today! Enjoy and share, please!
Flip flop wearers standing barefoot in airport security line. Gross people. Use protection. Put a sock on those feet! Nasty! 
All checked in for my Virgin America flight & having breakfast at Chili's. I am amazed at all the traveling flip-flop wearers. 
RT : Once again the  app excludes ASM Live events. Why, isn't it microbiology?
Once again the  app excludes ASM Live events. Why, isn't it microbiology?
well car is packed, coffee consumed on my way to FLL airport for. I woke up like a zombie this morning. Rest. Need more rest!
Talk for  is done! Looking forward to my session - Symbiosis as a driver of ecology & evolution. Now to upload... Grr
Looking forward to more playing with SRI's pathway-tools tomorrow. Fungal genomes loaded and ready to rock, also roll. 
Coming up at the  mtg. “The Great Indoors: Recent ...: The American Society for Microbiology meeting is ...
Talk to Wade Bell, just found out tonight he's a nematode guy RT I'll spend all of  talking about worms
Excited for  Jazzed to hear the latest and greatest in microbiology, and to reconnect with great microbiologists
Excited for . I'll be presenting some of my work on at poster 1128 on Monday from 10:45-12:30. Stop on by!
Excited to be attending this year's American Society for Microbiology General Meeting  
Fab 1st day in San Francisco.sealions,sun & ex max security prison!Pity my phone seems 2b on its way out.Hope can still tweet from 
Finally landed in San Francisco! Set up for  and a video shoot at the exploratorium. Then I see  for our!
I am just about finished packing for . On the AM flight from FLL. Hoping for a nice long transcontinental nap!
The A. Society for Microbiology and A. Society of Missiology are both meeting now using -- should be amusing.
My lab mates and PI's left for  this afternoon. Sad I won't be able to make it this year. have fun everyone!
 Last minute decision to attend  in SF on Sunday. I scored a room at the InterContinental on Hotwire for CHEAP!
Sharing faith isnt about convicting someone else. Its about being an instrument of truth. Where u r changed as much as anyone else
Maybe prophesy is all about timing... About to whom you say it, when, in what situation. 
Prophesy is the life giving imagination that a different life is possible.
"Christian prophecy is the power of weakness that unmasks the weakness of power." Kritzinger  
   I'll spend all of talking about worms--to convince people 2 join the Dark Side (euks)
Mission in... Basic ethos and values, bold humility, prophetic dialogue, saying YES, saying NO, living Yes and living No 
Music metaphors most helpful for talking about gospel in context. It is performed, interpreted, remixed in community. 
Ok people, I bit the bullet and will officially be attending for the day on Sunday - does this officially make me a microbiologist?
Mission in... The gospel is always in the culture, in the context. We must always interpret it. 
"Border-crossing encounters are fundamental to Christian epistemology." Kritzinger  
Summer course  over.  grant submitted. Off to San Francisco and  tomorrow. Fun times... 
Mission with... Patterns of encounter. We must stop with this idea that we are moving into empty space. 
Mission as... Unfolding initiatives and concrete things to do. Can anyone do all of this? We either narrow it down or choose one.
Stoked for  !! Plane delayed, practicing patience.
Dr. Klippies Kritzinger bringing opening: Mission as prophetic dialogue 
The Tree of Life: Coming up at the  mtg. "The Great Indoors: Recent Advances in the Ecology of Built…
American Academy of Microbiology poll on what challenges you think microbes should be used to solve 
Just finished planning my schedule for ; now for some wine and a little family time.
Great week at Steuebnville with@youthworks and my church Generations... Now in Chicago for  hope it's okay to tweet cuz I am!
Trying to come up w shorter term for "student, postdoc and young faculty members" of ASM. What do you think of "Next-gen members"? 
Checked into room; hotel provided chilled bottle of wine, cheese plate, & personal welcome note. I wonder who they think I am?
What's the tweeting protocol for ? Is it OK to tweet every talk?
 wondering:will the participants be at  or broadcasting from a sterile environment off-site? ;)
Sad to be missing  but better pizza , beer from  and tucks for boys. Plus, twitter updates from friends
Next year: bathrooms at ASM! MT  intrigued but disturbed at talk: Microbial Analysis of Environ Surfaces in Hotel Rooms
Hmm, intrigued but disturbed at this  livestreamed talk: Microbial Analysis of Environ Surfaces in Hotel Rooms
Missing my  friends. Those who are staying for ... hope to see you there.
PortEco and EcoCyc will be at booth 1623 at 
Arrived -SF - for  but am worried that this may not be the African Super Meateters meeting 
GAH!!! the  website is DOWN, and I need to look at the program to see if its worth me going. Anyone have a PDF copy??
Getting ready to leave for . I will be talking on Monday about engineering cyanobacteria.
Don't forget to register for the bioMérieux Scientific Symposium Monday night (18/6) at  
Seconded “ are you going to be Chief Microblogging Organizer at ? Consider this to be a nomination.”
 are you going to be Chief Microblogging Organizer at? Consider this to be a nomination.
We're excited to be in beautiful San Francisco next week for! Come visit us at booth No. 626. USB life science reagents 
In honor of today's events...I'm just going interrupt every speaker at mid talk
Last chance!  reception with  for SF editors & attendees. 6/18/2012 (open bar!!)
How can microbiol socs grow from medical micro emphasis to other global concerns eg environ quality?  
Shahana Kazmi, Pres of Pakistan Soc for Microbiol is updating us on impressive achievements of her Society  
Did you hear about our new PowerMag Soil & Microbial DNA Kits? Check it out  & stop by booth 1616 at for info.
Smart-Vue wireless monitoring solutions safeguard the integrity of your precious samples  Booth 1012 
Revco UxF freezers deliver ultimate protection and optimum capacity for your most critical samples  Booth 1012 
CryoExtra provide outstanding sample protection with uniform cryo temperatures  Booth 1012 
 Hi, tnx for following :) May I ask if you're at ?
Find out about the new  cold storage products on display at 's  on booth 1015. Details to follow…
 in SF on the 18th with Mindy, Marcia, Steve for - will we be seeing you? 10:30 - 4pm PDT
 carbon fiber rotors, adv alternative to metal rotors w/o fatigue or corrosion booth 1015  
Spending the day at  membership board meeting.
 Haha :) Best posters are those prepared at the last minute; Get to squeeze in your latest results. Good luck with it! Njoy
 When at  don't forget we're waiting to meet u 's booth-1625. Will u give us 10 minutes to show u our system?
Missed "Using next-gen seq for microbial genomes & transcriptomes"?- webinar recording now available 
We're hosting our annual Scientific Symposium at Monday June 18 at 6pm.  2 be keynote speaker. Don't miss it! Pls RT
Hey  Sun-Tue when skipping sessions  make use of the time by visiting 's booth 1625 ;) Our guys would love to meet
We're getting excited for  tomorrow! Visit booth 1213 and learn about all the content solutions Elsevier can provide!
How to get from SFO or Oakland airport to  MT  BART. It'll take you right there
I made it in one piece to SF, navigated to the BART station and I’m now sitting on a train full of what appear to be scientists 
What's the best way to get from the SFO airport to downtown?
Getting excited for  !  is taking over SF this weekend!! Visit booth 1616 June 17-19. Meet our scientists & get free samples!
Getting ready for  ! Stop by booth 1616 Sun-Tue and enter to win Beats by Dr. Dre Headphones & get free DNA & RNA kit samples!
 Integrated Biotherapeutics has a poster up on Sunday describing candidate staph vaccines. Stop on by! 
No booth for us at  so instead we'll have a twitter booth on drop us a tweet if you have queries/want to meet etc
Heading off to the Bay Area for a few days for  to connect with awesome client . Can't wait to see  and!
One day until  - already in the Bay area, who knows who I might run into?
  you guys at  tomorrow night? I'm getting in early afternoon.
We're excited to visit beautiful San Francisco next week for! Come visit us at booth n° 1301
Ok tweeps, who is presenting at  ? I have a poster Monday afternoon. Come say hi. It's awesome. 
American Society of Microbiology’s  starts tomorrow, stop by the  / USB booth 626 -
 - , booth 1415, and  will be there. We love feedback & tips. Hope to see you there!
Definitely coming for a coloring book! MT : Also, you can pick up the awesome Cell Press coloring book (booth 1211)
Plus pick up a Cell Press pin at Booth 1211, if you’re spotted with this you could win a Kindle. Too bad I can’t do this... 
Also, you can pick up a copy of the 2011 Best of Cell Host & Microbe and the awesome Cell Press coloring book  (booth 1211)
I’ll be at the Cell Press booth 1211 Monday June 18 and Tuesday June 19 from 1-2 - stop by and chat and pick up a copy of TIM
The Academy wants to know what global challenges you think microbiology should solve!  
Planning my itinerary and getting ready for . Hope to see you there!
American Society of Microbiology’s  starts tomorrow, stop by the  booth 1015
And there's Boston airport. Offline for the next 9 hours or so. here I come
 We're glad you like it! It makes it so easy to navigate the show. TriStar &  did a great job!
On my way to  (@ Washington Dulles International Airport (IAD) w/ 45 others) 
: Stop by  booth 727 2 talk nuc. acid purification, protein purif. from E. coli, quantitation w/fluorescence and more!
Attending the  in San Francisco? Map out your event in advance: Floor plan Lathrop Engineering Booth #523
Love the enthusiasm! RT : Omfg im going to San Francisco tomorrow! 
Your bugs, our box - see the anaerobic Bugbox workstation at booth 1534 
Congratulations to LaJoyce Debro who has been honored with the William A. Hinton Research Training Award.  ^lg
RT : MicrobiologyOpen meet-the-editor event:, June 17th, from 2-4pm, Booth 724. Free coffee, cookies and...
Leaving  after 5 great days of science. Now for San Francisco and !
MicrobiologyOpen meet-the-editor event: , June 17th, from 2-4pm, Booth 724. Free coffee, cookies and T-shirts! ...
MicrobiologyOpen meet-the-editor event:  from 3pm on the 17th June 
We'll be demoing MacVector 12.6 at the Come have a look and pick up a demo CD and a mouse pad! Booth 936
We'll be at  in SF from the 16-19th! Come see us at Booth 1131 to meet our researchers & take advantage of our samples & giveaways!
Attending ? Visit Promega representatives at booth# 727 and ask about our latest nucleic acid purification & quantitation products.
Learn about the wide range of reliable  peristaltic pumps at booth 1015  
RT : Rapid whole genome seq for neonatal MRSA investigation using . New article in  today: 
Congratulations to Bernhard Palsson who has won the  Research Award.   ^lg
Presenting at  or another conf? Here's some tips: How to Present Successfully at Conferences - Bitesize Bio
Attending  ? Stop by Booth #1617 for special savings on products for microbial 
Good advice! RT : Enroute to . Do NOT forget poster tube that has rolled to the far back of the overhead bin.
See you at  June 17-19!  is booth 1616. Details on our giveaway here (bottom of article) : 
Any suggestions for topics and/or speakers at the upcoming 6th Trends in Medical Mycology (TIMM6, 2013)  
Proud Prof! RT  My student Adam just found out he’s giving a talk @ . Short notice, great opportunity! Tue 11am Div. B/D mtg
Enroute to . Do NOT forget poster tube that has rolled to the far back of the overhead bin.
For anyone studying infectious disease, we will be offering two OMNIgene seminars following . Please retweet!
Using next-gen seq for microbial genomes &amp;amp; transcriptomes- webinar recording now available 
: Rapid whole genome seq for neonatal MRSA investigation using . In  today: ” 
RT : We're going to  in SF! Come meet us-booth 1625. We'll explain about   tool & give cool gifts
RT : We're going to  in SF! Come meet us-booth 1625. We'll explain about   tool & give cool gifts
RT : We're going to  in SF! Come meet us-booth 1625. We'll explain about   tool & give cool gifts
Rapid whole genome seq for neonatal MRSA investigation using. New article in  today: 
Tomorrow I pick up  and we're going to check out the zoos in Sacramento and SF before  
Look for  at ! How do you think that microbes can save the world? Let us know!
Our last batch of sample DNA & RNA isolation kits is beginning its journey to  stop by booth 1616 next week to try some free kits!
 Title is “Disruption of the Fas-FasL signaling pathway by the plasminogen activator protease of Yersinia pestis” 
My student Adam just found out he’s giving a talk at . Short notice, but a great opportunity! Stop by Tues. at 11 am, Div. B/D mtg.
New article in  today: Rapid whole genome seq for neonatal MRSA investigation using 
look fwd to it! MT : Novel Bacterial Taxa in the Human Microbiome  I'll be speaking about this at
Stay tuned! Over the next few days we'll announce new products to be displayed on booth 1015 at's 
Novel Bacterial Taxa in the Human Microbiome (Open): I'll be speaking about this at 
 I don't get to see much of GM but I'll be working ASM booth Mon pm Stop by! Also if u renew on site-free t-shirt!
Download the  floor plan to make sure you can find booth 1015 to speak with our  colleagues:
 The winner of the ABMM/ABMLI Professional Recognition Award is L. Barth Reller.  ^lg
We’ll be in SF this weekend for  (booth 840). Hope to see you there! We’ll be presenting OMNIgene, our infectious disease kit.
I will be evaluating technologies at  in Boston, then at in San Francisco. busy week ahead.
 I will be there tweeting my way through. Let's tweet up while we are there. 
Are you going to  - bring this flier to the Daigger booth and they will donate $5 to Seeding Labs
 Wow! Wonder if that's a record?!? Thanks for being a loyal  member. Hope to see you in San Fran!
 printing my poster, few more minutes & it'll be ready :) excited about celebrating my bd with friends in SF this year !!
Starting to pack for San Fransisco!  with the will be fun, but the  with will be awesome
 releases antifungal testing panel aligned to  M38 A2 reference method - will be featured at !
looking forward to learning about great technologies and research., San Francisco June 16-19
 Will U be at ? We're going, and would love to meet. Come by booth 1625, we'll show U  tool
Florida bound! Back in time for one day of . Deciding between Mon or Tues...
 Good luck with it . Stop by booth 1625. We'll be happy to hear how it went & show U  tool
 -come by booth 1625 & hear what is. It helps researchers manage their data & focus on science. CU there :)
 we'll be at  too! You're invited to our booth-1625. Check out   tool. We'd love to meet
Neutrophil in Immunity congres in Quebec was very nice. Now grant writing and saturday to the  in San Francisco
1 flight down, 1 (big one) to go. At LHR waiting for my connection to San Francisco for  and 
Hmmm, wonder if can take part in next Tues'  from ? I think the time difference will make it around lunchtime...
I'll be attending . First microbiology meeting for me, and looking forward to it!
(1) Technician returned ms to journal w responses to reviewers & compelling new data. (2)  poster printed. A satisfying day.
 exact same weekend of my birthday... Celebrating to the MAX !!
All these tweets about people having scheduled  already have me feeling complacent...haven't even written my talk yet
 Congratulations to the Scherago-Rubin Award winner Brent Barrett.   ^lg
ASMCUE/ASM scheduling finished. Happy to meet up with people depending on schedule. Hilton Union Square from 17-20 June.
Using  to make my poster for . The official quick-ref for this amazing free drawing program:







SPACER