Tag: MiSeq

Method of interest: precision sequencing of near full 16S rRNA genes on a MiSeq (from @Cath_B & @koadman)  

By Jonathan Eisen   Posted in .Featured Methods and Tools
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Definitely worth checking this one (from Burke and Darling) out if you do microbial diversity studies … Background The bacterial 16S rRNA gene has historically been used in defining bacterial taxonomy and phylogeny. However, there are currently no high-throughput methods to sequence full-length 16S rRNA genes present in a sample with precision. Results We describe …

Comparing the new 16S rRNA V4 and ITS primers to the old primers-RESULTS!

By Embriette Hyde   Posted in Bioinformatics Methods and Tools Research from microBEnet
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The Knight lab has been working hard testing new primers for 16S rRNA amplicon production and its time to share our progress. So far, the 16S rRNA V4 region forward primer (designated 515f) has been paired with five different reverse primers (806r, 926r, 967r, 1048r, and 1391r) to amplify ribosomal RNA from bacteria, Archaea, and …