Job: antibiotic resistance in 100k TB genomes

Hi All I’d just like to highlight a job we are advertising, to lead the bioinformatic (sequence+variation) analysis of 100,000 M tuberculosis genomes which we are sequencing (WGS), ~50,000 of which will be phenotyped for 12 drugs, and the remainder for some subset. Note the deadline is very soon – Monday 6th March! This project …

Characterize your metagenomic sequences faster than ever with PALADIN

With our paper recently accepted for publication and many months of practical application, we’re excited to spread the word about our new tool for characterizing metagenomes, PALADIN! Short for Protein ALignment And Detection INterface, PALADIN was our response to the inherent problem of limited references in shotgun metagenomic sequencing. While DNA barcoding involving 16S, 18S, …

From genomes to phenotypes: Traitar, the microbial trait analyzer

There is an increasing number of studies with a large number genomes recovered from isolate, metagenome, or single cell sequencing. To bridge the gap between the available genome sequences and available phenotype information, we have developed Traitar, a bioinformatics software to phenotype bacteria based on their genome sequence (see workflow below) . Traitar includes phenotype models for …

Microbiology of the Built Environment Meeting: Day 1

Here’s the report from Day 1 of the 5th annual Microbiology of the Built Environment meeting in Boulder, CO.  Following my summary of the talks is a Storify of all the tweets from the day. The first talk of the day was by Ulla Haverinen-Shaughnessy from the University of Eastern Finland whose talk was entitled …

Making tables in LaTeX

I’m working on a manuscript where we analyze OTU co-occurrence patterns using network analyses as well as the traditional approaches (ordination, alpha diversity, etc.). This means that we have to include some equations in our methods and so for the first time, I am using Authorea and LaTeX. I am getting used to working with the …

December Mothur Workshop, part 2

Here are my notes from day 2 of the Mothur workshop taught by Pat Schloss (pdschloss at gmail.com) in December 2015. For those who are interested in learning to use Mothur for microbiome studies, Pat will be teaching another one in February. Mothur is better for bacterial characterization than eukaryotes because the sequences are aligned before OTU clustering. This …