Quick post – this looks potentially very useful – ATLAS – comprehensive infrastructure for assembly, annotation, & genomic binning of metagenomic data 

OK I have not tried this out yet but this could be of interest. ATLAS (Automatic Tool for Local Assembly Structures) is a comprehensive multi-omics data analysis pipeline that is massively parallel and scalable. ATLAS contains a modular analysis pipeline for assembly, annotation, quantification and genome binning of metagenomics and metatranscriptomics data and a framework …

Job: antibiotic resistance in 100k TB genomes

Hi All I’d just like to highlight a job we are advertising, to lead the bioinformatic (sequence+variation) analysis of 100,000 M tuberculosis genomes which we are sequencing (WGS), ~50,000 of which will be phenotyped for 12 drugs, and the remainder for some subset. Note the deadline is very soon – Monday 6th March! This project …

Characterize your metagenomic sequences faster than ever with PALADIN

With our paper recently accepted for publication and many months of practical application, we’re excited to spread the word about our new tool for characterizing metagenomes, PALADIN! Short for Protein ALignment And Detection INterface, PALADIN was our response to the inherent problem of limited references in shotgun metagenomic sequencing. While DNA barcoding involving 16S, 18S, …

Of possible interest – DAS tool for metagenomic binning

Saw an interesting Tweet check out this novel metagenomics tool to reconstruct thousands of high-quality genomes @ChrisMKSieber #banfieldlab https://t.co/67bk8CbFmP — Alexander Probst (@AlexJProbst) February 13, 2017 And this tool may be of interest – it is from a new preprint in BioRXiv.  See abstract below: Microbial communities are critical to ecosystem function. A key objective …

16S rRNA Sequencing Using the Oxford Nanopore Minion

There has been some interest in our recent preprint describing Oxford Nanopore MinIONTM sequencing for 16S rRNA microbiome characterization and I was asked to write a post for microbenet on this technology. Disclaimers – this paper is a work in progress – our paper has not yet been peer-reviewed and we are continuing to revise …

What kind of DNA lingers on ATM keypads? Your food, your skin microbes…and (maybe) parasites

Amidst the November/December holiday chaos, myself and co-authors were proud to witness the publication of a neat new paper focused on ATM keypads in New York City. Yes, just like all other surfaces in the Built Environment, those ATM keypads are harboring lots of microbes and bits of orphaned DNA! This ATM keypad study was work that …

Undergraduate Research Opportunity in Microbiome Informatics at Oregon State University

The Department of Statistics at Oregon State University is hosting a summer REU (Research Experiences for Undergraduates) program that focuses on microbiome data analysis. This is a unique, paid research and training opportunity for STEM undergraduate students who want an interdisciplinary research experience in both biology and statistics. During a ten week training period, students will be …

Fungal diversity surveys not using ITS amplicons

I was at a meeting a few weeks ago where the topic of fungal diversity surveys was discussed and many people there commented on how ITS based surveys (one of the main approaches for culture independent studies of fungi) had some limitations. ITS stands for the “internal transcribed sequence” and it is a region in …

Swabs to Genomes Class: Summary and Course Materials

A few weeks ago we wrapped up Spring Quarter here at UC Davis and the end of our experimental “Swabs to Genomes” class, taught as a freshman seminar.   As we introduced here, the idea was to take a set of students from colonies on a plate (from a swab) through a collection of ready to …