Taxonomic Forensics in Metagenomics with In Silico Marker Panels

Introduction In 2013-2014, a metagenomics project called “Pathomap” collected 1,457 swab samples from the surfaces of all active subway stations throughout New York City (NYC), as well as samples from the Gowanus Canal and several parks. Each sample was sequenced to an average depth of 3.6 million reads (paired-end 125 nucleotides), generating a city-wide metagenomic …

Everything you wanted to know about space poop

Let’s face it, when microbiologists get together over beers 9/10 times the conversation ends up about poop.   Or maybe that’s just among the ones I know?  Feces provides the critical window into the important world of the gut microbiome,  a large percentage of the biomass in feces is microbial and who doesn’t love fecal transplants? …

Behind the scenes: Microbial biogeography of a university campus

Behind the scenes: Microbial biogeography of a university campus My name is Ashley Ross (@ashanneross) and, during my final year as an undergraduate student in the Department of Biology at the University of Waterloo, I began to wonder which microorganisms I was contacting on a daily basis while on campus. Every day on my way …

The Making of MetAnnotate

Blog post prepared jointly by Andrew Doxey (@acdoxey) and Josh Neufeld (@joshdneufeld) The “aquariome” Back in 2013, as part of a project assessing aquarium microbial communities and their role in nutrient cycling, Laura Sauder (graduate student in the Neufeld lab) sequenced a shotgun metagenomic library from a freshwater aquarium biofilter that was installed on this …

Fascinating look at the design of a science infographic (on life in dust)

Designers Martin Krzywinski and Barbara Jeannie Hunnicutt provide a peek behind the scenes, and explain how they developed a data visualization based on bacterial genome information derived from dust. Source: The Evolution of a Scientific American Infographic: Secret Life in Household Dust – Scientific American Blog Network This is an absolutely fascinating look behind the …

The Microbes in Grey Water

As buildings move forward in an attempt to become more “green,” it is important to continue to assess how the microbiology changes with these new systems. A recent study from Keely et al used high-throughput pyrosequencing to look at what microbes live in the different points in grey water systems. The sampling efforts were split into …

Who is in the operating room during c-section deliveries?

Infants born via c-section have a microbiome community composed mostly of skin bacteria [1-3], but the source of these skin bacteria is unknown. People quickly shed bacteria into their environment, leaving their own bacterial signature in a room within hours [4]. Do hospital operating rooms harbor skin bacteria that could colonize c-section delivered infants? A …

The Building Science and Microbiology Disconnect

Did you know that NASA took on the project of creating the world’s greenest building? It’s called Sustainability Base and it’s located right in the heart of Silicon Valley. It boasts incredible energy efficiency and often puts energy back into the grid. And as it’s website says, it’s claimed to have been built to be “furnished …