home Journal club New papers on Microbiology of the Built Environment, January 21, 2017

New papers on Microbiology of the Built Environment, January 21, 2017

 

Microbes in the house and office

A survey of xerophilic Aspergillus from indoor environment, including descriptions of two new section Aspergillus species producing eurotium-like sexual states – Cobus M. Visagie – MycoKeys (OA)

Aspergillus mallochii

Xerophilic fungi grow at low water activity or low equilibrium relative humidity and are an important part of the indoor fungal community, of which Aspergillus is one of the dominant genera. A survey of xerophilic fungi isolated from Canadian and Hawaiian house dust resulted in the isolation of 1039 strains; 296 strains belong to Aspergillus and represented 37 species. (…)  Aspergillus sect. Aspergillus (formerly called Eurotium) was one of the most predominant groups from house dust with nine species identified. (…) Among all strains, two species were found to be new and are introduced here as A. mallochii and A. megasporus. (…)

Exposure to Indoor Allergens in Different Residential Settings and Its Influence on IgE Sensitization in a Geographically Confined Austrian Cohort – Teresa Stemeseder – PLOS ONE (OA)

Allergen concentrations in house dust samples collected in households with and without pets.

Exposure to indoor allergens is crucial for IgE sensitization and development of allergic symptoms. Residential settings influence the allergen amount in house dust and hence allergic sensitization. Within this study, we investigated allergen exposure and molecule-based IgE levels in a geographically confined region and evaluated the impact of housing, pets and cleaning. 501 adolescents from Salzburg, Austria participated in this cross-sectional study. House dust samples were examined regarding major mite, cat, dog, and mold allergens using a multiplex assay. (…) In investigated house dust samples, the concentration of cat allergen was highest while the prevalence of mold allergens was very low. (…). Allergen exposure to indoor allergens was influenced by setting of homes. Living in a farm environment and having a cat at home showed a protective effect for IgE sensitization and allergies. This cross-sectional study in combination with hereditary and lifestyle factors enables development of risk schemes for a more efficient management and potential prevention of allergic diseases.

Six-day measurement of size-resolved indoor fluorescent bioaerosols of outdoor origin in an office – Yangyang Xie – Particuology ($35.95)

Graphical abstract

Indoor airborne bioaerosols of outdoor origin play an important role in determining the exposure of humans to bioaerosols because people spend most of their time indoors. However, there are few studies focusing on indoor bioaerosols originating from outdoors. In this study, indoor versus outdoor size-resolved concentrations and particle asymmetry factors of airborne fluorescent bioaerosols in an office room were measured continuously for 6 days (144 h) using a fluorescent bioaerosol detector. (…) Correlations between indoor and outdoor fluorescent bioaerosol concentrations show significant concentration-attenuation and a time lag during the study period. (…)

Emission rates and the personal cloud effect associated with particle release from the perihuman environment – D. Licina – Indoor Air ($6 to rent, $38 to own)

Preview

Inhalation exposure to elevated particulate matter levels is correlated with deleterious health and well-being outcomes. Despite growing evidence that identifies humans as sources of coarse airborne particles, the extent to which personal exposures are influenced by particle releases near occupants is unknown. In a controlled chamber, we monitored airborne total particle levels with high temporal and particle-size resolution for a range of simulated occupant activities. We also sampled directly from the subject’s breathing zone to characterize exposures. A material-balance model showed that a sitting occupant released 8 million particles/h in the diameter range 1-10 μm. Elevated emissions were associated with increased intensity of upper body movements and with walking. (…) Manipulating papers and clothing fabric was a strong source of airborne particles. An increase in personal exposure was observed owing to particle mass exchange associated with a second room occupant.

Microbes in the hospital

 

Microbes on the farm

Housing Systems Influence Gut Microbiota Composition of Sows but Not of Their Piglets – Tereza Kubasova – PLOS ONE (OA)

Microbiota composition in sows, 1- and 4-day-old piglets.

Different housing systems can be used in pig production and little is known about their effect on gut microbiota composition. In this study we characterized fecal microbiota by sequencing the rRNA genes in sows kept during gestation in conventional pens with a slatted floor and in enriched pens with a floor covered with deep straw. After farrowing, microbiota of 1- and 4-day-old piglets were also monitored. Microbiota of sows from the enriched system contained significantly more Prevotella, Parabacteroides, CF231, Phascolarctobacterium, Fibrobacter, Anaerovibrio and YRC22 and significantly less Lactobacillus, Bulleidia, Lachnospira, Dorea, Ruminococcus and Oscillospira than microbiota of sows from the conventional system. (…) The production system therefore influenced microbiota composition, most likely due the ingestion of the straw. The microbiota of 1- and 4-day-old piglets differed from the microbiota of sows and sows therefore did not represent the most important source for their colonization in early days of life.

Bacterial Communities Associated with Houseflies (Musca domestica L.) Sampled within and between Farms – Simon Bahrndorff – PLOS ONE (OA)

Phylogenetic tree and heatmap of selected families

The housefly feeds and reproduces in animal manure and decaying organic substances and thus lives in intimate association with various microorganisms including human pathogens. In order to understand the variation and association between bacteria and the housefly, we used 16S rRNA gene amplicon sequencing to describe bacterial communities of 90 individual houseflies collected within and between ten dairy farms in Denmark. (…) Comparison of the microbiota of houseflies revealed a highly diverse microbiota compared to other insect species and with most variation in species richness and diversity found between individuals, but not locations. Our study is the first in-depth amplicon sequencing study of the housefly microbiota, and collectively shows that the microbiota of single houseflies is highly diverse and differs between individuals likely to reflect the lifestyle of the housefly. We suggest that these results should be taken into account when addressing the transmission of pathogens by the housefly and assessing the vector competence variation under natural conditions.

Microbial diversity in different compartments of an aquaponics system – Zala Schmautz – Archives of Microbiology ($39.95)

A small, portable aquaponics system. Source: Wikipedia.

Aquaponics is a solution for sustainable production of fish and plants in a single semi-closed system, where nutrient-rich water from the aquaculture provides nutrients for plant growth. We examined the microbial communities within an experimental aquaponics system. Whereas the fish feces contained a separate community dominated by bacteria of the genus Cetobacterium, the samples from plant roots, biofilter, and periphyton were more similar to each other, while the communities were more diverse. Detailed examination of the data gave the first indications to functional groups of organisms in the different compartments of the aquaponic system. As other nitrifiers other than members of the genus Nitrospira were only present at low numbers, it was anticipated that Nitrospirae may perform the nitrification process in the biofilm.

 

Microbes in space

MELiSSA the minimal biosphere: Human life, waste and refuge in deep space – Jeremy Walker – Futures ($41.95; but available at ResearchGate)

The MELiSSA Loop

MELiSSA (Micro-Ecological Life Support System Alternative) is a long-term technology program of the European Space Agency. Its aim is to construct autonomous habitats in deep space, supplying astronauts with fresh air, water and food through continuous microbial recycling of human wastes. This article considers how anticipated futures of space travel and environmental survival are materialized in the project to engineer the minimal biosphere capable of reliably sustaining human life: a human/microbe association with the fewest possible species. (…) MELiSSA researchers think of themselves as pragmatic enablers of space exploration, yet a wider family of space colonization projects are now imagined in terms of the prospect that the Earth might cease to function as the minimal biosphere capable of supporting civilisation. MELiSSA’s politics of anticipation are paradoxical, promising technologies with which to escape from the Earth and through which it may be sustained.

Microbes in the city

Soil microbial communities are shaped by vegetation type and park age in cities under cold climate – Nan Hui – Environmental Microbiology ($6 to rent, #38 to own)

East City Park complete with a squirrel, Moscow, Idaho. Source: Wikipedia.

Soil microbes play a key role in controlling ecosystem functions and providing ecosystem services. Yet, microbial communities in urban green space soils remain poorly characterized. Here we compared soil microbial communities in 41 urban parks of (i) divergent plant functional types (evergreen trees, deciduous trees, lawn) and (ii) different ages (constructed 10, ∼50, and >100 years ago). These microbial communities were also compared to those in 5 control forests in southern Finland. Our results indicate that, despite frequent disturbances in urban parks, urban soil microbes still followed the classic patterns typical of plant-microbe associations in natural environments: both bacterial and fungal communities in urban parks responded to plant functional groups, but fungi were under tighter control of plants than bacteria. We show that park age shaped the composition of microbial communities, possibly because vegetation in old parks have had a longer time to modify soil properties and microbial communities than in young parks. (…).

Microbes in drinking water

Effect of temperature and colonization of Legionella pneumophila and Vermamoeba vermiformis on bacterial community composition of copper drinking water biofilms – Helen Y. Buse – Microbial Biotechnology (OA)

NMDS ordination plots and major OTU contributors for biofilm samples.

It is unclear how the water-based pathogen, Legionella pneumophila (Lp), and associated free-living amoeba (FLA) hosts change or are changed by the microbial composition of drinking water (DW) biofilm communities. Thus, this study characterized the bacterial community structure over a 7-month period within mature (> 600-day-old) copper DW biofilms in reactors simulating premise plumbing and assessed the impact of temperature and introduction of Lp and its FLA host, Vermamoeba vermiformis (Vv), co-cultures (LpVv). (…) Two-way PERMANOVA and PCoA revealed that temperature was a major driver of microbiome diversity. Biofilm community composition also changed over the seven-month period and could be associated with significant shifts in dissolved oxygen, alkalinity and various metals in the influent DW. Hence, temperature, biofilm age, DW quality and transient intrusions/amplification of pathogens and FLA hosts may significantly impact biofilm microbiomes and modulate pathogen levels over extended periods.

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