home Bioinformatics, Sloan Microbiology of the Built Environment Program What sequencing core do you recommend for environmental samples with low concentrations?

What sequencing core do you recommend for environmental samples with low concentrations?

Have you ever wondered what’s in the air of a daycare center, where kids swap microbes every day? A team from Virginia Tech and the University of Pittsburgh is investigating the viral ecology in air of a daycare center using metagenomics. We have collected samples on HVAC filters and are looking for a sequencing core with HiSeq capabilities, that has experience handling environmental samples with low DNA/RNA concentrations in which targeted sequencing is not possible. The sample extracts are ~50 microliters (ul) in volume with a concentration of ~0.1 nanograms per microliter (ng/ul). If you have any suggestions, please leave a comment.

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Linsey Marr

Linsey Marr is an engineering professor at Virginia Tech studying the air quality and airborne disease transmission.

5 thoughts on “What sequencing core do you recommend for environmental samples with low concentrations?

  1. Thanks to everyone that has replied. We have done some initial tests using an Nextra XT kit with success (and agree that lots of negative controls are critical!) Our main question now is to figure out who does high quality metagenomic sequencing.

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